Prognostic significance and immunological role of HPRT1 in human cancers
Hypoxanthine phosphoribosyl transferase 1 (HPRT1), once considered a housekeeping gene, has been identified as playing an important role in several tumors. Its role in pan-cancer, however, has not been systematically studied. This study evaluates the relationship between HPRT1 and clinical paramete...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2024-03-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/9775 |
| _version_ | 1797261383582089216 |
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| author | Yiwen Lu Ruixue Chen Han Zhang Xu Sun Xiangjun Li Mengyuan Yang Xudong Zhang |
| author_facet | Yiwen Lu Ruixue Chen Han Zhang Xu Sun Xiangjun Li Mengyuan Yang Xudong Zhang |
| author_sort | Yiwen Lu |
| collection | DOAJ |
| description |
Hypoxanthine phosphoribosyl transferase 1 (HPRT1), once considered a housekeeping gene, has been identified as playing an important role in several tumors. Its role in pan-cancer, however, has not been systematically studied. This study evaluates the relationship between HPRT1 and clinical parameters, survival prognosis, and tumor immunity based on multi omics data from the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases. Drug sensitivity analysis screened 16 effective drugs against HPRT1, exploring the interactions with chemicals and genes. The significance of HPRT1 in tumor immunotherapy has also been investigated. Immunohistochemistry confirmed significant differences in the expression of HPRT1 between five tumor types (colon adenocarcinoma [COAD], head-neck squamous cell carcinoma [HNSC], lung adenocarcinoma [LUAD], thyroid carcinoma [THCA], and uterine corpus endometrial carcinoma [UCEC]) and adjacent normal tissues (P < 0.05). HPRT1 competitive endogenous RNA network was constructed in HNSC. Through cytological experiments, it was verified that HPRT1 plays a carcinogenic role in HNSC and is associated with tumor cell proliferation, migration, invasion, and apoptosis. In addition, there was a significant positive correlation between HPRT1 and programmed cell death-1 (PD-1) expression in HNSC (P < 0.05). These findings suggest that HPRT1 may be a potential biomarker for predicting and treating cancer.
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| first_indexed | 2024-04-24T23:40:21Z |
| format | Article |
| id | doaj.art-a059e0d7de244f1f9c27c741d5d27436 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:40:21Z |
| publishDate | 2024-03-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-a059e0d7de244f1f9c27c741d5d274362024-03-15T13:21:04ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2024-03-0124210.17305/bb.2023.9775Prognostic significance and immunological role of HPRT1 in human cancersYiwen Lu0Ruixue Chen1Han Zhang2Xu Sun3Xiangjun Li4Mengyuan Yang5Xudong Zhang6Department of Oral and Maxillofacial Surgery, Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang, ChinaDepartment of Oral and Maxillofacial Surgery, Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang, ChinaDepartment of Pathology, Shijiazhuang Great Wall Hospital of Integrated Traditional Chinese and Western Medicine, Shijiazhuang, China Department of Oral and Maxillofacial Surgery, Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang, ChinaDepartment of Oral and Maxillofacial Surgery, Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang, ChinaDepartment of Oral and Maxillofacial Surgery, Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang, ChinaDepartment of Oral and Maxillofacial Surgery, Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang, China Hypoxanthine phosphoribosyl transferase 1 (HPRT1), once considered a housekeeping gene, has been identified as playing an important role in several tumors. Its role in pan-cancer, however, has not been systematically studied. This study evaluates the relationship between HPRT1 and clinical parameters, survival prognosis, and tumor immunity based on multi omics data from the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases. Drug sensitivity analysis screened 16 effective drugs against HPRT1, exploring the interactions with chemicals and genes. The significance of HPRT1 in tumor immunotherapy has also been investigated. Immunohistochemistry confirmed significant differences in the expression of HPRT1 between five tumor types (colon adenocarcinoma [COAD], head-neck squamous cell carcinoma [HNSC], lung adenocarcinoma [LUAD], thyroid carcinoma [THCA], and uterine corpus endometrial carcinoma [UCEC]) and adjacent normal tissues (P < 0.05). HPRT1 competitive endogenous RNA network was constructed in HNSC. Through cytological experiments, it was verified that HPRT1 plays a carcinogenic role in HNSC and is associated with tumor cell proliferation, migration, invasion, and apoptosis. In addition, there was a significant positive correlation between HPRT1 and programmed cell death-1 (PD-1) expression in HNSC (P < 0.05). These findings suggest that HPRT1 may be a potential biomarker for predicting and treating cancer. https://www.bjbms.org/ojs/index.php/bjbms/article/view/9775Pan-cancer, hypoxanthine phosphoribosyl transferase 1 (HPRT1), immunity, cancer risk factors, prognosis |
| spellingShingle | Yiwen Lu Ruixue Chen Han Zhang Xu Sun Xiangjun Li Mengyuan Yang Xudong Zhang Prognostic significance and immunological role of HPRT1 in human cancers Biomolecules & Biomedicine Pan-cancer, hypoxanthine phosphoribosyl transferase 1 (HPRT1), immunity, cancer risk factors, prognosis |
| title | Prognostic significance and immunological role of HPRT1 in human cancers |
| title_full | Prognostic significance and immunological role of HPRT1 in human cancers |
| title_fullStr | Prognostic significance and immunological role of HPRT1 in human cancers |
| title_full_unstemmed | Prognostic significance and immunological role of HPRT1 in human cancers |
| title_short | Prognostic significance and immunological role of HPRT1 in human cancers |
| title_sort | prognostic significance and immunological role of hprt1 in human cancers |
| topic | Pan-cancer, hypoxanthine phosphoribosyl transferase 1 (HPRT1), immunity, cancer risk factors, prognosis |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/9775 |
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