Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in <i>Bupleurum chinense</i> DC. Roots
<i>Bupleurum chinense</i> is an important medicinal plant in China; however, little is known regarding how this plant transcribes and synthesizes saikosaponins under drought stress. Herein, we investigated how drought stress stimulates the transcriptional changes of <i>B. chinense&...
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MDPI AG
2022-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/17/5470 |
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| author | Linlin Yang Lu Qiao Xiuhong Su Baoyu Ji Chengming Dong |
| author_facet | Linlin Yang Lu Qiao Xiuhong Su Baoyu Ji Chengming Dong |
| author_sort | Linlin Yang |
| collection | DOAJ |
| description | <i>Bupleurum chinense</i> is an important medicinal plant in China; however, little is known regarding how this plant transcribes and synthesizes saikosaponins under drought stress. Herein, we investigated how drought stress stimulates the transcriptional changes of <i>B. chinense</i> to synthesize saikosaponins. Short-term drought stress induced the accumulation of saikosaponins, especially from the first re-watering stage (RD_1 stage) to the second re-watering stage (RD_2 stage). Saikosaponin-a and saikosaponin-d increased by 84.60% and 75.13%, respectively, from the RD_1 stage to the RD_2 stage. Drought stress also stimulated a rapid increase in the levels of the hormones abscisic acid, salicylic acid, and jasmonic acid. We screened 49 Unigenes regarding the terpenoid backbone and triterpenoid biosynthesis, of which 33 differential genes were significantly up-regulated during drought stress. Moreover, one P450 and two UGTs are possibly involved in the synthesis of saikosaponins, while some transcription factors may be involved in regulating the expression of key enzyme genes. Our study provides a reference for the cultivation of <i>B. chinense</i> and a practical means to ensure the quality (safety and effectiveness) of <i>B. chinense</i> for medicinal use, as well as insights into the modernization of the China Agriculture Research System. |
| first_indexed | 2024-03-10T01:30:49Z |
| format | Article |
| id | doaj.art-a05a084466cc4788b09ff7eac05e0231 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:30:49Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a05a084466cc4788b09ff7eac05e02312023-11-23T13:42:32ZengMDPI AGMolecules1420-30492022-08-012717547010.3390/molecules27175470Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in <i>Bupleurum chinense</i> DC. RootsLinlin Yang0Lu Qiao1Xiuhong Su2Baoyu Ji3Chengming Dong4Henan Provincial Ecological Planting Engineering Technology Research Center of Daodi Herbs, School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaHenan Provincial Ecological Planting Engineering Technology Research Center of Daodi Herbs, School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaHenan Provincial Ecological Planting Engineering Technology Research Center of Daodi Herbs, School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaHenan Provincial Ecological Planting Engineering Technology Research Center of Daodi Herbs, School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaHenan Provincial Ecological Planting Engineering Technology Research Center of Daodi Herbs, School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China<i>Bupleurum chinense</i> is an important medicinal plant in China; however, little is known regarding how this plant transcribes and synthesizes saikosaponins under drought stress. Herein, we investigated how drought stress stimulates the transcriptional changes of <i>B. chinense</i> to synthesize saikosaponins. Short-term drought stress induced the accumulation of saikosaponins, especially from the first re-watering stage (RD_1 stage) to the second re-watering stage (RD_2 stage). Saikosaponin-a and saikosaponin-d increased by 84.60% and 75.13%, respectively, from the RD_1 stage to the RD_2 stage. Drought stress also stimulated a rapid increase in the levels of the hormones abscisic acid, salicylic acid, and jasmonic acid. We screened 49 Unigenes regarding the terpenoid backbone and triterpenoid biosynthesis, of which 33 differential genes were significantly up-regulated during drought stress. Moreover, one P450 and two UGTs are possibly involved in the synthesis of saikosaponins, while some transcription factors may be involved in regulating the expression of key enzyme genes. Our study provides a reference for the cultivation of <i>B. chinense</i> and a practical means to ensure the quality (safety and effectiveness) of <i>B. chinense</i> for medicinal use, as well as insights into the modernization of the China Agriculture Research System.https://www.mdpi.com/1420-3049/27/17/5470<i>Bupleurum chinense</i> DC.drought stressterpenoid backbone and triterpenoid biosynthesisplant hormonessaikosaponins |
| spellingShingle | Linlin Yang Lu Qiao Xiuhong Su Baoyu Ji Chengming Dong Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in <i>Bupleurum chinense</i> DC. Roots Molecules <i>Bupleurum chinense</i> DC. drought stress terpenoid backbone and triterpenoid biosynthesis plant hormones saikosaponins |
| title | Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in <i>Bupleurum chinense</i> DC. Roots |
| title_full | Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in <i>Bupleurum chinense</i> DC. Roots |
| title_fullStr | Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in <i>Bupleurum chinense</i> DC. Roots |
| title_full_unstemmed | Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in <i>Bupleurum chinense</i> DC. Roots |
| title_short | Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in <i>Bupleurum chinense</i> DC. Roots |
| title_sort | drought stress stimulates the terpenoid backbone and triterpenoid biosynthesis pathway to promote the synthesis of saikosaponin in i bupleurum chinense i dc roots |
| topic | <i>Bupleurum chinense</i> DC. drought stress terpenoid backbone and triterpenoid biosynthesis plant hormones saikosaponins |
| url | https://www.mdpi.com/1420-3049/27/17/5470 |
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