Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma
The aim of presented research is to develop a simple and quick method of spectrophotometric detection for the determination of doxorubicin hydrochloride in blood and plasma. Anthracycline antibiotics are among the most effective antineoplastic agents. However, despite their high efficacy in the trea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/2/112 |
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| author | Tomasz Sikora Karolina Morawska Wiesław Lisowski Paweł Rytel Agnieszka Dylong |
| author_facet | Tomasz Sikora Karolina Morawska Wiesław Lisowski Paweł Rytel Agnieszka Dylong |
| author_sort | Tomasz Sikora |
| collection | DOAJ |
| description | The aim of presented research is to develop a simple and quick method of spectrophotometric detection for the determination of doxorubicin hydrochloride in blood and plasma. Anthracycline antibiotics are among the most effective antineoplastic agents. However, despite their high efficacy in the treatment of various types of cancer, their administration is limited primarily because they exhibit myocardial toxicity. This may be a limiting factor in the dosage of medications; nevertheless, drugs exhibiting this mechanism of action constitute a very important group of chemotherapeutics. One of the more widely studied antibiotics from the anthracycline group is doxorubicin. It exhibits the highest antineoplastic activity from among a number of derivative compounds. Because of the adverse effects of doxorubicin, especially cardiotoxicity, it is important to maintain control of its concentration in body fluids. The method in the study consists of extraction doxorubicin from the plasma or blood and measurements of the absorbance of light in the visible light range in a DOX solution with respect to a reference sample. The research used blood and plasma samples spiked with doxorubicin to give concentrations in the range of 0.2–10 µg/mL. Obtained LODs were 1.6 µg/mL and 1.2 µg/mL, respectively. |
| first_indexed | 2024-03-09T21:16:07Z |
| format | Article |
| id | doaj.art-a05d400b09ff4cd780d3ef1caf5bf545 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T21:16:07Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-a05d400b09ff4cd780d3ef1caf5bf5452023-11-23T21:33:13ZengMDPI AGPharmaceuticals1424-82472022-01-0115211210.3390/ph15020112Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and PlasmaTomasz Sikora0Karolina Morawska1Wiesław Lisowski2Paweł Rytel3Agnieszka Dylong4Military Institute of Chemistry and Radiometry, 00-910 Warsaw, PolandMilitary Institute of Chemistry and Radiometry, 00-910 Warsaw, PolandMilitary Institute of Chemistry and Radiometry, 00-910 Warsaw, PolandMilitary Institute of Chemistry and Radiometry, 00-910 Warsaw, PolandMilitary Institute of Engineer Technology Named after Professor Józef Kosacki, 50-961 Wroclaw, PolandThe aim of presented research is to develop a simple and quick method of spectrophotometric detection for the determination of doxorubicin hydrochloride in blood and plasma. Anthracycline antibiotics are among the most effective antineoplastic agents. However, despite their high efficacy in the treatment of various types of cancer, their administration is limited primarily because they exhibit myocardial toxicity. This may be a limiting factor in the dosage of medications; nevertheless, drugs exhibiting this mechanism of action constitute a very important group of chemotherapeutics. One of the more widely studied antibiotics from the anthracycline group is doxorubicin. It exhibits the highest antineoplastic activity from among a number of derivative compounds. Because of the adverse effects of doxorubicin, especially cardiotoxicity, it is important to maintain control of its concentration in body fluids. The method in the study consists of extraction doxorubicin from the plasma or blood and measurements of the absorbance of light in the visible light range in a DOX solution with respect to a reference sample. The research used blood and plasma samples spiked with doxorubicin to give concentrations in the range of 0.2–10 µg/mL. Obtained LODs were 1.6 µg/mL and 1.2 µg/mL, respectively.https://www.mdpi.com/1424-8247/15/2/112anticancer drugsdoxorubicinUV-Vis spectroscopydrug monitoringdoxorubicin quantification |
| spellingShingle | Tomasz Sikora Karolina Morawska Wiesław Lisowski Paweł Rytel Agnieszka Dylong Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma Pharmaceuticals anticancer drugs doxorubicin UV-Vis spectroscopy drug monitoring doxorubicin quantification |
| title | Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma |
| title_full | Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma |
| title_fullStr | Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma |
| title_full_unstemmed | Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma |
| title_short | Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma |
| title_sort | application of optical methods for determination of concentration of doxorubicin in blood and plasma |
| topic | anticancer drugs doxorubicin UV-Vis spectroscopy drug monitoring doxorubicin quantification |
| url | https://www.mdpi.com/1424-8247/15/2/112 |
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