Screening for Zika virus RNA in sera of suspected cases: a retrospective cross-sectional study
Abstract Background Zika virus (ZIKV) became a global human health concern owing to its rapid spread worldwide and its association with congenital and neurological disorders. The current epidemiological profile of arboviruses in Brazil is characterized by widespread co-circulation of Dengue virus, C...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12985-018-1070-z |
| _version_ | 1819145729150550016 |
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| author | Lívia Sacchetto Danielle Alves Gomes Zauli Galileu Barbosa Costa Sarah Anne J Guagliardo Luige Biciati Alvim Feliciana Lage de Oliveira Marinho Jônatas Santos Abrahão Giliane de Souza Trindade Erna Geessien Kroon Elvis Cristian Cueva Mateo Betânia Paiva Drumond |
| author_facet | Lívia Sacchetto Danielle Alves Gomes Zauli Galileu Barbosa Costa Sarah Anne J Guagliardo Luige Biciati Alvim Feliciana Lage de Oliveira Marinho Jônatas Santos Abrahão Giliane de Souza Trindade Erna Geessien Kroon Elvis Cristian Cueva Mateo Betânia Paiva Drumond |
| author_sort | Lívia Sacchetto |
| collection | DOAJ |
| description | Abstract Background Zika virus (ZIKV) became a global human health concern owing to its rapid spread worldwide and its association with congenital and neurological disorders. The current epidemiological profile of arboviruses in Brazil is characterized by widespread co-circulation of Dengue virus, Chikungunya virus, and ZIKV throughout the country. These viruses cause acute diseases frequently with overlapping symptoms, which could result in an inaccurate diagnosis based solely on clinical and epidemiological grounds. Here we conducted a screening for ZIKV RNA in serum samples from patients across Brazil with suspected ZIKV infection. Methods Using RT-qPCR, we investigated ZIKV RNA in 3001 serum samples. Samples were passively acquired through a private laboratory network, between December 2015 and August 2016, from 27 Brazilian Federative Units. We performed descriptive statistics on demographic variables including sex, age, and geographic location. Results ZIKV was detected in 11.4% (95%CI = 10.3–12.6%) of the sera. ZIKV RNA was detected in sera collected throughout the country, but during the analyzed period, RNA was more frequently detected in samples from the Southeast, Midwest, and North regions (3.9 to 5.8 times higher) when compared to the Northeast and South regions. Conclusions These data reinforce the importance of laboratory diagnosis, surveillance systems, and further epidemiological studies to understand the dynamics of outbreaks and diseases associated with ZIKV and other arboviruses. |
| first_indexed | 2024-12-22T13:02:39Z |
| format | Article |
| id | doaj.art-a05f1219191147c5b800edeb0cdf9e69 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-22T13:02:39Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-a05f1219191147c5b800edeb0cdf9e692022-12-21T18:24:57ZengBMCVirology Journal1743-422X2018-10-011511810.1186/s12985-018-1070-zScreening for Zika virus RNA in sera of suspected cases: a retrospective cross-sectional studyLívia Sacchetto0Danielle Alves Gomes Zauli1Galileu Barbosa Costa2Sarah Anne J Guagliardo3Luige Biciati Alvim4Feliciana Lage de Oliveira Marinho5Jônatas Santos Abrahão6Giliane de Souza Trindade7Erna Geessien Kroon8Elvis Cristian Cueva Mateo9Betânia Paiva Drumond10Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisInstituto Hermes PardiniLaboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisPoxvirus and Rabies Branch, US Centers for Disease Control and PreventionInstituto Hermes PardiniInstituto Hermes PardiniLaboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisLaboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisLaboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisInstituto Hermes PardiniLaboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisAbstract Background Zika virus (ZIKV) became a global human health concern owing to its rapid spread worldwide and its association with congenital and neurological disorders. The current epidemiological profile of arboviruses in Brazil is characterized by widespread co-circulation of Dengue virus, Chikungunya virus, and ZIKV throughout the country. These viruses cause acute diseases frequently with overlapping symptoms, which could result in an inaccurate diagnosis based solely on clinical and epidemiological grounds. Here we conducted a screening for ZIKV RNA in serum samples from patients across Brazil with suspected ZIKV infection. Methods Using RT-qPCR, we investigated ZIKV RNA in 3001 serum samples. Samples were passively acquired through a private laboratory network, between December 2015 and August 2016, from 27 Brazilian Federative Units. We performed descriptive statistics on demographic variables including sex, age, and geographic location. Results ZIKV was detected in 11.4% (95%CI = 10.3–12.6%) of the sera. ZIKV RNA was detected in sera collected throughout the country, but during the analyzed period, RNA was more frequently detected in samples from the Southeast, Midwest, and North regions (3.9 to 5.8 times higher) when compared to the Northeast and South regions. Conclusions These data reinforce the importance of laboratory diagnosis, surveillance systems, and further epidemiological studies to understand the dynamics of outbreaks and diseases associated with ZIKV and other arboviruses.http://link.springer.com/article/10.1186/s12985-018-1070-zZika virusCross-sectional studyEpidemiologyBrazilDengue virusChikungunya virus |
| spellingShingle | Lívia Sacchetto Danielle Alves Gomes Zauli Galileu Barbosa Costa Sarah Anne J Guagliardo Luige Biciati Alvim Feliciana Lage de Oliveira Marinho Jônatas Santos Abrahão Giliane de Souza Trindade Erna Geessien Kroon Elvis Cristian Cueva Mateo Betânia Paiva Drumond Screening for Zika virus RNA in sera of suspected cases: a retrospective cross-sectional study Virology Journal Zika virus Cross-sectional study Epidemiology Brazil Dengue virus Chikungunya virus |
| title | Screening for Zika virus RNA in sera of suspected cases: a retrospective cross-sectional study |
| title_full | Screening for Zika virus RNA in sera of suspected cases: a retrospective cross-sectional study |
| title_fullStr | Screening for Zika virus RNA in sera of suspected cases: a retrospective cross-sectional study |
| title_full_unstemmed | Screening for Zika virus RNA in sera of suspected cases: a retrospective cross-sectional study |
| title_short | Screening for Zika virus RNA in sera of suspected cases: a retrospective cross-sectional study |
| title_sort | screening for zika virus rna in sera of suspected cases a retrospective cross sectional study |
| topic | Zika virus Cross-sectional study Epidemiology Brazil Dengue virus Chikungunya virus |
| url | http://link.springer.com/article/10.1186/s12985-018-1070-z |
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