Generation of High-Value Genomic Resource in Rice: A “Subgenomic Library” of Low-Light Tolerant Rice Cultivar Swarnaprabha
Rice is the major staple food crop for more than 50% of the world’s total population, and its production is of immense importance for global food security. As a photophilic plant, its yield is governed by the quality and duration of light. Like all photosynthesizing plants, rice perceives the change...
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MDPI AG
2023-03-01
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| author | Sovanlal Sahu Payal Gupta Thirumalanahalli Prakash Gowtham Kumar Shiva Yogesh Tenkabailu Dharmanna Sanjay Ayushi Singh Hay Van Duong Sharat Kumar Pradhan Deepak Singh Bisht Nagendra Kumar Singh Mirza J. Baig Rhitu Rai Prasanta K. Dash |
| author_facet | Sovanlal Sahu Payal Gupta Thirumalanahalli Prakash Gowtham Kumar Shiva Yogesh Tenkabailu Dharmanna Sanjay Ayushi Singh Hay Van Duong Sharat Kumar Pradhan Deepak Singh Bisht Nagendra Kumar Singh Mirza J. Baig Rhitu Rai Prasanta K. Dash |
| author_sort | Sovanlal Sahu |
| collection | DOAJ |
| description | Rice is the major staple food crop for more than 50% of the world’s total population, and its production is of immense importance for global food security. As a photophilic plant, its yield is governed by the quality and duration of light. Like all photosynthesizing plants, rice perceives the changes in the intensity of environmental light using phytochromes as photoreceptors, and it initiates a morphological response that is termed as the shade-avoidance response (SAR). Phytochromes (PHYs) are the most important photoreceptor family, and they are primarily responsible for the absorption of the red (R) and far-red (FR) spectra of light. In our endeavor, we identified the morphological differences between two contrasting cultivars of rice: IR-64 (low-light susceptible) and Swarnaprabha (low-light tolerant), and we observed the phenological differences in their growth in response to the reduced light conditions. In order to create genomic resources for low-light tolerant rice, we constructed a subgenomic library of Swarnaprabha that expedited our efforts to isolate light-responsive photoreceptors. The titer of the library was found to be 3.22 × 10<sup>5</sup> cfu/mL, and the constructed library comprised clones of 4–9 kb in length. The library was found to be highly efficient as per the number of recombinant clones. The subgenomic library will serve as a genomic resource for the Gramineae community to isolate photoreceptors and other genes from rice. |
| first_indexed | 2024-03-11T06:54:44Z |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T06:54:44Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-a062d26f59a94cb6aeb27537563c80a22023-11-17T09:42:04ZengMDPI AGBiology2079-77372023-03-0112342810.3390/biology12030428Generation of High-Value Genomic Resource in Rice: A “Subgenomic Library” of Low-Light Tolerant Rice Cultivar SwarnaprabhaSovanlal Sahu0Payal Gupta1Thirumalanahalli Prakash Gowtham2Kumar Shiva Yogesh3Tenkabailu Dharmanna Sanjay4Ayushi Singh5Hay Van Duong6Sharat Kumar Pradhan7Deepak Singh Bisht8Nagendra Kumar Singh9Mirza J. Baig10Rhitu Rai11Prasanta K. Dash12ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, IndiaICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, IndiaICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, IndiaICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, IndiaICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, IndiaICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, IndiaICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, IndiaICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, IndiaICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, IndiaRice is the major staple food crop for more than 50% of the world’s total population, and its production is of immense importance for global food security. As a photophilic plant, its yield is governed by the quality and duration of light. Like all photosynthesizing plants, rice perceives the changes in the intensity of environmental light using phytochromes as photoreceptors, and it initiates a morphological response that is termed as the shade-avoidance response (SAR). Phytochromes (PHYs) are the most important photoreceptor family, and they are primarily responsible for the absorption of the red (R) and far-red (FR) spectra of light. In our endeavor, we identified the morphological differences between two contrasting cultivars of rice: IR-64 (low-light susceptible) and Swarnaprabha (low-light tolerant), and we observed the phenological differences in their growth in response to the reduced light conditions. In order to create genomic resources for low-light tolerant rice, we constructed a subgenomic library of Swarnaprabha that expedited our efforts to isolate light-responsive photoreceptors. The titer of the library was found to be 3.22 × 10<sup>5</sup> cfu/mL, and the constructed library comprised clones of 4–9 kb in length. The library was found to be highly efficient as per the number of recombinant clones. The subgenomic library will serve as a genomic resource for the Gramineae community to isolate photoreceptors and other genes from rice.https://www.mdpi.com/2079-7737/12/3/428ricephotoreceptorsphytochromesfar-red lightlight-responsive genesGramineae |
| spellingShingle | Sovanlal Sahu Payal Gupta Thirumalanahalli Prakash Gowtham Kumar Shiva Yogesh Tenkabailu Dharmanna Sanjay Ayushi Singh Hay Van Duong Sharat Kumar Pradhan Deepak Singh Bisht Nagendra Kumar Singh Mirza J. Baig Rhitu Rai Prasanta K. Dash Generation of High-Value Genomic Resource in Rice: A “Subgenomic Library” of Low-Light Tolerant Rice Cultivar Swarnaprabha Biology rice photoreceptors phytochromes far-red light light-responsive genes Gramineae |
| title | Generation of High-Value Genomic Resource in Rice: A “Subgenomic Library” of Low-Light Tolerant Rice Cultivar Swarnaprabha |
| title_full | Generation of High-Value Genomic Resource in Rice: A “Subgenomic Library” of Low-Light Tolerant Rice Cultivar Swarnaprabha |
| title_fullStr | Generation of High-Value Genomic Resource in Rice: A “Subgenomic Library” of Low-Light Tolerant Rice Cultivar Swarnaprabha |
| title_full_unstemmed | Generation of High-Value Genomic Resource in Rice: A “Subgenomic Library” of Low-Light Tolerant Rice Cultivar Swarnaprabha |
| title_short | Generation of High-Value Genomic Resource in Rice: A “Subgenomic Library” of Low-Light Tolerant Rice Cultivar Swarnaprabha |
| title_sort | generation of high value genomic resource in rice a subgenomic library of low light tolerant rice cultivar swarnaprabha |
| topic | rice photoreceptors phytochromes far-red light light-responsive genes Gramineae |
| url | https://www.mdpi.com/2079-7737/12/3/428 |
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