Identification of a frit-related sample carryover in newborn screening by tandem mass spectrometry
The need for high-throughput analysis of multiple analytes for inborn errors of metabolism in newborn screening (NBS) has led to the introduction of tandem mass spectrometry (MS/MS) into the NBS laboratory. In a flow-injection analysis (FIA), the predominant MS/MS method utilized for NBS, samples ar...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Journal of Mass Spectrometry and Advances in the Clinical Lab |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667145X23000019 |
| _version_ | 1797936325599428608 |
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| author | Kyunghun Kim Howon Lee Jeong Joong Lee Kyoungho Cha Nam Hyun Park Young Keun Shin Hyojin Chae Eun-Jee Oh |
| author_facet | Kyunghun Kim Howon Lee Jeong Joong Lee Kyoungho Cha Nam Hyun Park Young Keun Shin Hyojin Chae Eun-Jee Oh |
| author_sort | Kyunghun Kim |
| collection | DOAJ |
| description | The need for high-throughput analysis of multiple analytes for inborn errors of metabolism in newborn screening (NBS) has led to the introduction of tandem mass spectrometry (MS/MS) into the NBS laboratory. In a flow-injection analysis (FIA), the predominant MS/MS method utilized for NBS, samples are introduced directly into the mass spectrometer without chromatographic separation. When a high-throughput FIA-based MS/MS method is implemented on newer generations of mass spectrometers with increased sensitivity, the risk of carryover and contamination increases. In the present study, we report the carryover of ornithine identified during the implementation of the NeoBase™ 2 (PerkinElmer) non-derivatized kits on the Xevo-TQD platform (Waters Corporation) and describe the source of the carryover, which was traced to the stainless-steel frit-type inline filter. Furthermore, a possible compound-dependent interaction with the stainless-steel frit is suggested based on the structure of ornithine and its effect on separation techniques. Investigation and mitigation of carryover can be a time and resource consuming process, and to this end, our report on identification of a stainless-steel frit as the source of delayed elution and carryover of ornithine should be recognized as a rare, albeit possible source of carryover in FIA-MS/MS methods adopted for NST. |
| first_indexed | 2024-04-10T18:29:03Z |
| format | Article |
| id | doaj.art-a06380c1d9ce43bebebb235a96d78867 |
| institution | Directory Open Access Journal |
| issn | 2667-145X |
| language | English |
| last_indexed | 2024-04-10T18:29:03Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Mass Spectrometry and Advances in the Clinical Lab |
| spelling | doaj.art-a06380c1d9ce43bebebb235a96d788672023-02-02T04:50:52ZengElsevierJournal of Mass Spectrometry and Advances in the Clinical Lab2667-145X2023-01-01275660Identification of a frit-related sample carryover in newborn screening by tandem mass spectrometryKyunghun Kim0Howon Lee1Jeong Joong Lee2Kyoungho Cha3Nam Hyun Park4Young Keun Shin5Hyojin Chae6Eun-Jee Oh7Department of Laboratory Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Laboratory Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Laboratory Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Laboratory Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaBio-Medical Science Co., Ltd, Seoul, Republic of KoreaWaters Korea Ltd, Seoul, Republic of KoreaDepartment of Laboratory Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Corresponding author at: Department of Laboratory Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea.Department of Laboratory Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaThe need for high-throughput analysis of multiple analytes for inborn errors of metabolism in newborn screening (NBS) has led to the introduction of tandem mass spectrometry (MS/MS) into the NBS laboratory. In a flow-injection analysis (FIA), the predominant MS/MS method utilized for NBS, samples are introduced directly into the mass spectrometer without chromatographic separation. When a high-throughput FIA-based MS/MS method is implemented on newer generations of mass spectrometers with increased sensitivity, the risk of carryover and contamination increases. In the present study, we report the carryover of ornithine identified during the implementation of the NeoBase™ 2 (PerkinElmer) non-derivatized kits on the Xevo-TQD platform (Waters Corporation) and describe the source of the carryover, which was traced to the stainless-steel frit-type inline filter. Furthermore, a possible compound-dependent interaction with the stainless-steel frit is suggested based on the structure of ornithine and its effect on separation techniques. Investigation and mitigation of carryover can be a time and resource consuming process, and to this end, our report on identification of a stainless-steel frit as the source of delayed elution and carryover of ornithine should be recognized as a rare, albeit possible source of carryover in FIA-MS/MS methods adopted for NST.http://www.sciencedirect.com/science/article/pii/S2667145X23000019OrnithineCarryoverTandem mass spectrometryFlow injection analysisNewborn screening |
| spellingShingle | Kyunghun Kim Howon Lee Jeong Joong Lee Kyoungho Cha Nam Hyun Park Young Keun Shin Hyojin Chae Eun-Jee Oh Identification of a frit-related sample carryover in newborn screening by tandem mass spectrometry Journal of Mass Spectrometry and Advances in the Clinical Lab Ornithine Carryover Tandem mass spectrometry Flow injection analysis Newborn screening |
| title | Identification of a frit-related sample carryover in newborn screening by tandem mass spectrometry |
| title_full | Identification of a frit-related sample carryover in newborn screening by tandem mass spectrometry |
| title_fullStr | Identification of a frit-related sample carryover in newborn screening by tandem mass spectrometry |
| title_full_unstemmed | Identification of a frit-related sample carryover in newborn screening by tandem mass spectrometry |
| title_short | Identification of a frit-related sample carryover in newborn screening by tandem mass spectrometry |
| title_sort | identification of a frit related sample carryover in newborn screening by tandem mass spectrometry |
| topic | Ornithine Carryover Tandem mass spectrometry Flow injection analysis Newborn screening |
| url | http://www.sciencedirect.com/science/article/pii/S2667145X23000019 |
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