MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3
Shengliang Sun,1,* Lei Fu,1,* Gen Wang,1 Jianli Wang,1 Liping Xu2 1Department of Orthopedic Trauma, The 89th Army Hospital of the Chinese People’s Liberation Army, Weifang, Shandong, People’s Republic of China; 2Department of Oncology, The 89th Army Hospital of the Chinese People...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-09-01
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| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/microrna-431-5p-inhibits-the-tumorigenesis-of-osteosarcoma-through-tar-peer-reviewed-article-CMAR |
| _version_ | 1819277102970568704 |
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| author | Sun S Fu L Wang G Wang J Xu L |
| author_facet | Sun S Fu L Wang G Wang J Xu L |
| author_sort | Sun S |
| collection | DOAJ |
| description | Shengliang Sun,1,* Lei Fu,1,* Gen Wang,1 Jianli Wang,1 Liping Xu2 1Department of Orthopedic Trauma, The 89th Army Hospital of the Chinese People’s Liberation Army, Weifang, Shandong, People’s Republic of China; 2Department of Oncology, The 89th Army Hospital of the Chinese People’s Liberation Army, Weifang, Shandong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Liping Xu Tel +86 536-5019053Email xuliping1680@163.comPurpose: This study aimed to evaluate the regulatory role of miR-431-5p on the tumorigenesis of osteosarcoma (OS) and the underlying mechanism involving pannexin 3 (PANX3).Methods: qRT-PCR was applied to measure the expression of miR-431-5p in OS tissues and cells. PANX3 and miR-431-5p were overexpressed in U2OS and HOS cells. The cell viability and apoptosis were determined by MTT and FITC/PI double staining assay, respectively. Transwell assay was performed to detect cell migration and invasion. The protein expression of cleave-caspase-3 and MMP-2/-9 was detected by Western blot. The target relationship between miR-431-5p and PANX3 was predicated by ENCORI and identified by DLR assay. The anti-tumor effect of miR-431-5p was further analyzed in a xenograft tumor model in mice.Results: MiR-431-5p expression was down-regulated in OS tissues and negatively correlated with lymph node metastasis and TNM stage. Over-expression of miR-431-5p induced cell apoptosis, inhibited cell proliferation, migration and invasion, up-regulated cleave-caspase-3, and down-regulated MMP-2 and -9 in OS cells. Over-expression of miR-431-5p also inhibited the growth of tumor xenografts in mice. In addition, PANX3 was a target of miR-431-5p. Over-expression of PANX3 reversed the anti-tumor effect of miR-431-5p mimics on U2OS and HOS cells.Conclusion: Up-regulation of miR-431-5p suppressed the tumorigenesis of OS via targeting PANX3.Keywords: osteosarcoma, microRNA-431-5p, pannexin 3, proliferation, migration |
| first_indexed | 2024-12-23T23:50:47Z |
| format | Article |
| id | doaj.art-a063e986f2944e35b7dd957bdf491004 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-23T23:50:47Z |
| publishDate | 2020-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-a063e986f2944e35b7dd957bdf4910042022-12-21T17:25:24ZengDove Medical PressCancer Management and Research1179-13222020-09-01Volume 128159816956851MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3Sun SFu LWang GWang JXu LShengliang Sun,1,* Lei Fu,1,* Gen Wang,1 Jianli Wang,1 Liping Xu2 1Department of Orthopedic Trauma, The 89th Army Hospital of the Chinese People’s Liberation Army, Weifang, Shandong, People’s Republic of China; 2Department of Oncology, The 89th Army Hospital of the Chinese People’s Liberation Army, Weifang, Shandong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Liping Xu Tel +86 536-5019053Email xuliping1680@163.comPurpose: This study aimed to evaluate the regulatory role of miR-431-5p on the tumorigenesis of osteosarcoma (OS) and the underlying mechanism involving pannexin 3 (PANX3).Methods: qRT-PCR was applied to measure the expression of miR-431-5p in OS tissues and cells. PANX3 and miR-431-5p were overexpressed in U2OS and HOS cells. The cell viability and apoptosis were determined by MTT and FITC/PI double staining assay, respectively. Transwell assay was performed to detect cell migration and invasion. The protein expression of cleave-caspase-3 and MMP-2/-9 was detected by Western blot. The target relationship between miR-431-5p and PANX3 was predicated by ENCORI and identified by DLR assay. The anti-tumor effect of miR-431-5p was further analyzed in a xenograft tumor model in mice.Results: MiR-431-5p expression was down-regulated in OS tissues and negatively correlated with lymph node metastasis and TNM stage. Over-expression of miR-431-5p induced cell apoptosis, inhibited cell proliferation, migration and invasion, up-regulated cleave-caspase-3, and down-regulated MMP-2 and -9 in OS cells. Over-expression of miR-431-5p also inhibited the growth of tumor xenografts in mice. In addition, PANX3 was a target of miR-431-5p. Over-expression of PANX3 reversed the anti-tumor effect of miR-431-5p mimics on U2OS and HOS cells.Conclusion: Up-regulation of miR-431-5p suppressed the tumorigenesis of OS via targeting PANX3.Keywords: osteosarcoma, microRNA-431-5p, pannexin 3, proliferation, migrationhttps://www.dovepress.com/microrna-431-5p-inhibits-the-tumorigenesis-of-osteosarcoma-through-tar-peer-reviewed-article-CMARosteosarcomamicrorna-431-5ppannexin 3proliferationmigration |
| spellingShingle | Sun S Fu L Wang G Wang J Xu L MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 Cancer Management and Research osteosarcoma microrna-431-5p pannexin 3 proliferation migration |
| title | MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 |
| title_full | MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 |
| title_fullStr | MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 |
| title_full_unstemmed | MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 |
| title_short | MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 |
| title_sort | microrna 431 5p inhibits the tumorigenesis of osteosarcoma through targeting panx3 |
| topic | osteosarcoma microrna-431-5p pannexin 3 proliferation migration |
| url | https://www.dovepress.com/microrna-431-5p-inhibits-the-tumorigenesis-of-osteosarcoma-through-tar-peer-reviewed-article-CMAR |
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