Involvement of p.R72P and PIN3 Ins16bp (TP53) Polymorphisms and the I157T (CHEK2) Mutation in Breast Cancer Occurrence in Burkina Faso
Introduction: The TP53 and CHEK2 genes have been described as breast cancer susceptibility genes and some of their polymorphisms have been associated with an increased risk of breast cancer in certain populations. Aim: The objective of this study was to investigate the p.R72P and PIN3 Ins16bp (TP53)...
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| Format: | Article |
| Language: | English |
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West Asia Organization for Cancer Prevention
2023-07-01
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| Series: | Asian Pacific Journal of Cancer Biology |
| Subjects: | |
| Online Access: | http://www.waocp.com/journal/index.php/apjcb/article/view/1083 |
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| author | Soayebo Dabre Abdou Azaque Zoure Touwendpoulimdé Isabelle Kiendrébéogo Nayi Zongo Lanyo Jospin Amegnona Herman Karim Sombie Marc Donald Wilfried Adico Bélélé Siméon Bakyono Lassina Traoré Teega-Wendé Clarisse Ouedraogo Rogomenoma Alice Ouedraogo Théodora M. Zohoncon Albert Théophane Yonli Alexis Yobi Sawadogo Florencia W. Djigma Jacques Simpore |
| author_facet | Soayebo Dabre Abdou Azaque Zoure Touwendpoulimdé Isabelle Kiendrébéogo Nayi Zongo Lanyo Jospin Amegnona Herman Karim Sombie Marc Donald Wilfried Adico Bélélé Siméon Bakyono Lassina Traoré Teega-Wendé Clarisse Ouedraogo Rogomenoma Alice Ouedraogo Théodora M. Zohoncon Albert Théophane Yonli Alexis Yobi Sawadogo Florencia W. Djigma Jacques Simpore |
| author_sort | Soayebo Dabre |
| collection | DOAJ |
| description | Introduction: The TP53 and CHEK2 genes have been described as breast cancer susceptibility genes and some of their polymorphisms have been associated with an increased risk of breast cancer in certain populations.
Aim: The objective of this study was to investigate the p.R72P and PIN3 Ins16bp (TP53) polymorphisms and the I157T (CHEK2) mutation developping of breast cancer.
Methods: This case-control study had enrolled 144 participants including 65 cases (breast cancer patients) and 79 controls (women without breast abnormalities) in the city of Ouagadougou in Burkina Faso. The DNA was extracted using the method of “salting out” and the genotyping of polymorphisms was performed by ASO-PCR (Allele Specific Oligonucleotides - Polymerase Chain Reaction), conventional PCR and PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) techniques.
Results: The heterozygous genotype (RP) of the p.R72P polymorphism of TP53 gene was in the majority in cases (73.85%) and controls (73.42%). Regarding to the PIN3 Ins16bp polymorphism of TP53 gene, the homozygous wild type (A1A1) was the most represented in both cases (53.85%) and controls (60.76%). Concerning the I157T mutation of CHEK2 gene, only one (01) patient was homozygous mutant (TT) and no controls had the mutation. This study found no association between these polymorphisms and the risk of breast cancer occurrence (p.R72P (OR=0.96; 95%IC (0.59-1.56); p=0.471), PIN3 Ins16bp (OR= 1.1; 95%IC (0.61-1.98); p=0.420)).
Conclusion: This study showed that the P allele of the p.R72P polymorphism and the wild-type allele (A1) of the PIN3 Ins16bp polymorphism were in the majority. The I157T mutation was very rare. These polymorphisms were not associated with the risk of developing breast cancer in this study. |
| first_indexed | 2024-03-08T09:36:02Z |
| format | Article |
| id | doaj.art-a067f851a9404343bae0029698ab0871 |
| institution | Directory Open Access Journal |
| issn | 2538-4635 |
| language | English |
| last_indexed | 2024-03-08T09:36:02Z |
| publishDate | 2023-07-01 |
| publisher | West Asia Organization for Cancer Prevention |
| record_format | Article |
| series | Asian Pacific Journal of Cancer Biology |
| spelling | doaj.art-a067f851a9404343bae0029698ab08712024-01-30T09:44:25ZengWest Asia Organization for Cancer PreventionAsian Pacific Journal of Cancer Biology2538-46352023-07-018213514510.31557/apjcb.2023.8.2.135-1451083Involvement of p.R72P and PIN3 Ins16bp (TP53) Polymorphisms and the I157T (CHEK2) Mutation in Breast Cancer Occurrence in Burkina FasoSoayebo Dabre0Abdou Azaque Zoure1Touwendpoulimdé Isabelle Kiendrébéogo2Nayi Zongo3Lanyo Jospin Amegnona4Herman Karim Sombie5Marc Donald Wilfried Adico6Bélélé Siméon Bakyono7Lassina Traoré8Teega-Wendé Clarisse Ouedraogo9Rogomenoma Alice Ouedraogo10Théodora M. Zohoncon11Albert Théophane Yonli12Alexis Yobi Sawadogo13Florencia W. Djigma14Jacques Simpore15Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso. Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso. Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Biomedical Research Laboratory (LaReBio), Biomedical and Public Health Department, Institute for Research in Health Sciences (IRSS/CNRST), 03 BP 7192 Ouaga03, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso.Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Department of General and Digestive Surgery, University Hospital Centre-Yalgado Ouedraogo, UFR/SDS, University Joseph KI-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso.Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso. Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso. Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso. Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso. Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso. Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso. Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Centre Universitaire de Gaoua, Nazi Boni University, 01 BP 1091 Bobo-Dioulasso 01. Burkina Faso.Centre Universitaire de Gaoua, Nazi Boni University, 01 BP 1091 Bobo-Dioulasso 01. Burkina Faso.Faculty of Medicine, Saint Thomas Aquinas University (USTA), 06 BP 10212 Ouagadougou 06, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso. Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Service of Gynecology, University Hospital Centre-Bogodogo, University Joseph KI-Zerbo, UFR/SDS, 03 BP 7021 Ouagadougou 03, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso. Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Laboratory of Molecular and Genetic Biology (Labiogene), Joseph KI-Zerbo University, 03 BP 7021 Ouagadougou 03, Burkina Faso. Pietro Annigoni Biomolecular Research Center (Cerba), 01 BP 364 Ouagadougou 01, Burkina Faso.Faculty of Medicine, Saint Thomas Aquinas University (USTA), 06 BP 10212 Ouagadougou 06, Burkina Faso.Introduction: The TP53 and CHEK2 genes have been described as breast cancer susceptibility genes and some of their polymorphisms have been associated with an increased risk of breast cancer in certain populations. Aim: The objective of this study was to investigate the p.R72P and PIN3 Ins16bp (TP53) polymorphisms and the I157T (CHEK2) mutation developping of breast cancer. Methods: This case-control study had enrolled 144 participants including 65 cases (breast cancer patients) and 79 controls (women without breast abnormalities) in the city of Ouagadougou in Burkina Faso. The DNA was extracted using the method of “salting out” and the genotyping of polymorphisms was performed by ASO-PCR (Allele Specific Oligonucleotides - Polymerase Chain Reaction), conventional PCR and PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) techniques. Results: The heterozygous genotype (RP) of the p.R72P polymorphism of TP53 gene was in the majority in cases (73.85%) and controls (73.42%). Regarding to the PIN3 Ins16bp polymorphism of TP53 gene, the homozygous wild type (A1A1) was the most represented in both cases (53.85%) and controls (60.76%). Concerning the I157T mutation of CHEK2 gene, only one (01) patient was homozygous mutant (TT) and no controls had the mutation. This study found no association between these polymorphisms and the risk of breast cancer occurrence (p.R72P (OR=0.96; 95%IC (0.59-1.56); p=0.471), PIN3 Ins16bp (OR= 1.1; 95%IC (0.61-1.98); p=0.420)). Conclusion: This study showed that the P allele of the p.R72P polymorphism and the wild-type allele (A1) of the PIN3 Ins16bp polymorphism were in the majority. The I157T mutation was very rare. These polymorphisms were not associated with the risk of developing breast cancer in this study.http://www.waocp.com/journal/index.php/apjcb/article/view/1083breast cancer, tp53, chek2, polymorphisms, burkina faso. |
| spellingShingle | Soayebo Dabre Abdou Azaque Zoure Touwendpoulimdé Isabelle Kiendrébéogo Nayi Zongo Lanyo Jospin Amegnona Herman Karim Sombie Marc Donald Wilfried Adico Bélélé Siméon Bakyono Lassina Traoré Teega-Wendé Clarisse Ouedraogo Rogomenoma Alice Ouedraogo Théodora M. Zohoncon Albert Théophane Yonli Alexis Yobi Sawadogo Florencia W. Djigma Jacques Simpore Involvement of p.R72P and PIN3 Ins16bp (TP53) Polymorphisms and the I157T (CHEK2) Mutation in Breast Cancer Occurrence in Burkina Faso Asian Pacific Journal of Cancer Biology breast cancer, tp53, chek2, polymorphisms, burkina faso. |
| title | Involvement of p.R72P and PIN3 Ins16bp (TP53) Polymorphisms and the I157T (CHEK2) Mutation in Breast Cancer Occurrence in Burkina Faso |
| title_full | Involvement of p.R72P and PIN3 Ins16bp (TP53) Polymorphisms and the I157T (CHEK2) Mutation in Breast Cancer Occurrence in Burkina Faso |
| title_fullStr | Involvement of p.R72P and PIN3 Ins16bp (TP53) Polymorphisms and the I157T (CHEK2) Mutation in Breast Cancer Occurrence in Burkina Faso |
| title_full_unstemmed | Involvement of p.R72P and PIN3 Ins16bp (TP53) Polymorphisms and the I157T (CHEK2) Mutation in Breast Cancer Occurrence in Burkina Faso |
| title_short | Involvement of p.R72P and PIN3 Ins16bp (TP53) Polymorphisms and the I157T (CHEK2) Mutation in Breast Cancer Occurrence in Burkina Faso |
| title_sort | involvement of p r72p and pin3 ins16bp tp53 polymorphisms and the i157t chek2 mutation in breast cancer occurrence in burkina faso |
| topic | breast cancer, tp53, chek2, polymorphisms, burkina faso. |
| url | http://www.waocp.com/journal/index.php/apjcb/article/view/1083 |
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