Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replication
IntroductionDeformed wing virus (DWV) is one of the causative agents of colony collapse disorder. The structural protein of DWV plays a vital role in the process of viral invasion and host infection; however, there is limited research on DWV.Methods and ResultsIn this study, we screened the host pro...
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1096306/full |
| _version_ | 1811169503812255744 |
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| author | Li Sun Ming Li Yueyu Ma Sichao Huang Mingxiao Ma Dongliang Fei |
| author_facet | Li Sun Ming Li Yueyu Ma Sichao Huang Mingxiao Ma Dongliang Fei |
| author_sort | Li Sun |
| collection | DOAJ |
| description | IntroductionDeformed wing virus (DWV) is one of the causative agents of colony collapse disorder. The structural protein of DWV plays a vital role in the process of viral invasion and host infection; however, there is limited research on DWV.Methods and ResultsIn this study, we screened the host protein snapin, which can interact with the VP2 protein of DWV, using the yeast two-hybrid system. Through computer simulation and GST pull-down and CO-IP assays, an interaction between snapin and VP2 was confirmed. Furthermore, immunofluorescence and co-localization experiments revealed that VP2 and snapin primarily co-localized in the cytoplasm. Consequently, RNAi was used to interfere with the expression of snapin in worker bees to examine the replication of DWV after the interference. After silencing of snapin, the replication of DWV in worker bees was significantly downregulated. Hence, we speculated that snapin was associated with DWV infection and involved in at least one stage of the viral life cycle. Finally, we used an online server to predict the interaction domains between VP2 and snapin, and the results indicate that the interaction domain of VP2 was approximately located at 56–90, 136–145, 184–190, and 239–242 aa and the snapin interaction domain was approximately located at 31–54 and 115–136 aa.ConclusionThis research confirmed that DWV VP2 protein could interacts with the snapin of host protein, which provides a theoretical basis for further investigation of its pathogenesis and development of targeted therapeutic drugs. |
| first_indexed | 2024-04-10T16:43:29Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T16:43:29Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-a06bb8d4fa674562b3297230aa47396a2023-02-08T04:31:20ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.10963061096306Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replicationLi Sun0Ming Li1Yueyu Ma2Sichao Huang3Mingxiao Ma4Dongliang Fei5College of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University, Jinzhou, ChinaCollege of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University, Jinzhou, ChinaCollege of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University, Jinzhou, ChinaDepartment of Microbiology, Harbin Medical University, Harbin, ChinaCollege of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University, Jinzhou, ChinaCollege of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University, Jinzhou, ChinaIntroductionDeformed wing virus (DWV) is one of the causative agents of colony collapse disorder. The structural protein of DWV plays a vital role in the process of viral invasion and host infection; however, there is limited research on DWV.Methods and ResultsIn this study, we screened the host protein snapin, which can interact with the VP2 protein of DWV, using the yeast two-hybrid system. Through computer simulation and GST pull-down and CO-IP assays, an interaction between snapin and VP2 was confirmed. Furthermore, immunofluorescence and co-localization experiments revealed that VP2 and snapin primarily co-localized in the cytoplasm. Consequently, RNAi was used to interfere with the expression of snapin in worker bees to examine the replication of DWV after the interference. After silencing of snapin, the replication of DWV in worker bees was significantly downregulated. Hence, we speculated that snapin was associated with DWV infection and involved in at least one stage of the viral life cycle. Finally, we used an online server to predict the interaction domains between VP2 and snapin, and the results indicate that the interaction domain of VP2 was approximately located at 56–90, 136–145, 184–190, and 239–242 aa and the snapin interaction domain was approximately located at 31–54 and 115–136 aa.ConclusionThis research confirmed that DWV VP2 protein could interacts with the snapin of host protein, which provides a theoretical basis for further investigation of its pathogenesis and development of targeted therapeutic drugs.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1096306/fulldeformed wing virusVP2 proteinsnapininteractionviral replication |
| spellingShingle | Li Sun Ming Li Yueyu Ma Sichao Huang Mingxiao Ma Dongliang Fei Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replication Frontiers in Microbiology deformed wing virus VP2 protein snapin interaction viral replication |
| title | Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replication |
| title_full | Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replication |
| title_fullStr | Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replication |
| title_full_unstemmed | Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replication |
| title_short | Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replication |
| title_sort | interaction between the vp2 protein of deformed wing virus and host snapin protein and its effect on viral replication |
| topic | deformed wing virus VP2 protein snapin interaction viral replication |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1096306/full |
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