Fibroblasts Mediate Ectopic Bone Formation of Calcium Phosphate Ceramics
Heterogeneity of fibroblasts directly affects the outcome of tissue regeneration; however, whether bioactive ceramics regulate bone regeneration through fibroblasts is unclear. Ectopic bone formation model with biphasic calcium phosphate (BCP) implantation was used to investigate the temporal and sp...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/7/2569 |
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| author | Liangliang Fu Qin Zhao Jiaojiao Li Zifan Zhao Min Wang Huifang Sun Haibin Xia |
| author_facet | Liangliang Fu Qin Zhao Jiaojiao Li Zifan Zhao Min Wang Huifang Sun Haibin Xia |
| author_sort | Liangliang Fu |
| collection | DOAJ |
| description | Heterogeneity of fibroblasts directly affects the outcome of tissue regeneration; however, whether bioactive ceramics regulate bone regeneration through fibroblasts is unclear. Ectopic bone formation model with biphasic calcium phosphate (BCP) implantation was used to investigate the temporal and spatial distribution of fibroblasts around ceramics. The effect of BCP on L929 fibroblasts was evaluated by EdU assay, transwell assay, and qRT-PCR. Further, the effect of its conditioned medium on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was confirmed by ALP staining. SEM and XRD results showed that BCP contained abundant micro- and macro-pores and consisted of hydrogen-apatite (HA) and β-tricalcium phosphate (β-TCP) phases. Subsequently, BCP implanted into mice muscle successfully induced osteoblasts and bone formation. Fibroblasts labelled by vimentin gathered around BCP at 7 days and peaked at 14 days post implantation. In vitro, BCP inhibited proliferation of L929 fibroblast but promoted its migration. Moreover, expression of <i>Col1a1</i>, <i>Bmp2</i>, and <i>Igf1</i> in L929 treated by BCP increased significantly while expression of <i>Tgfb1</i> and <i>Acta</i> did not change. ALP staining further showed conditioned media from L929 fibroblasts treated by BCP could enhance osteogenic differentiation of BMSCs. In conclusion, fibroblasts mediate ectopic bone formation of calcium phosphate ceramics. |
| first_indexed | 2024-03-09T11:40:28Z |
| format | Article |
| id | doaj.art-a06df13b5f7744068936504ad1b0ad26 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T11:40:28Z |
| publishDate | 2022-03-01 |
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| series | Materials |
| spelling | doaj.art-a06df13b5f7744068936504ad1b0ad262023-11-30T23:33:57ZengMDPI AGMaterials1996-19442022-03-01157256910.3390/ma15072569Fibroblasts Mediate Ectopic Bone Formation of Calcium Phosphate CeramicsLiangliang Fu0Qin Zhao1Jiaojiao Li2Zifan Zhao3Min Wang4Huifang Sun5Haibin Xia6The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaHeterogeneity of fibroblasts directly affects the outcome of tissue regeneration; however, whether bioactive ceramics regulate bone regeneration through fibroblasts is unclear. Ectopic bone formation model with biphasic calcium phosphate (BCP) implantation was used to investigate the temporal and spatial distribution of fibroblasts around ceramics. The effect of BCP on L929 fibroblasts was evaluated by EdU assay, transwell assay, and qRT-PCR. Further, the effect of its conditioned medium on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was confirmed by ALP staining. SEM and XRD results showed that BCP contained abundant micro- and macro-pores and consisted of hydrogen-apatite (HA) and β-tricalcium phosphate (β-TCP) phases. Subsequently, BCP implanted into mice muscle successfully induced osteoblasts and bone formation. Fibroblasts labelled by vimentin gathered around BCP at 7 days and peaked at 14 days post implantation. In vitro, BCP inhibited proliferation of L929 fibroblast but promoted its migration. Moreover, expression of <i>Col1a1</i>, <i>Bmp2</i>, and <i>Igf1</i> in L929 treated by BCP increased significantly while expression of <i>Tgfb1</i> and <i>Acta</i> did not change. ALP staining further showed conditioned media from L929 fibroblasts treated by BCP could enhance osteogenic differentiation of BMSCs. In conclusion, fibroblasts mediate ectopic bone formation of calcium phosphate ceramics.https://www.mdpi.com/1996-1944/15/7/2569bone regenerationcalcium phosphate ceramicsfibroblast |
| spellingShingle | Liangliang Fu Qin Zhao Jiaojiao Li Zifan Zhao Min Wang Huifang Sun Haibin Xia Fibroblasts Mediate Ectopic Bone Formation of Calcium Phosphate Ceramics Materials bone regeneration calcium phosphate ceramics fibroblast |
| title | Fibroblasts Mediate Ectopic Bone Formation of Calcium Phosphate Ceramics |
| title_full | Fibroblasts Mediate Ectopic Bone Formation of Calcium Phosphate Ceramics |
| title_fullStr | Fibroblasts Mediate Ectopic Bone Formation of Calcium Phosphate Ceramics |
| title_full_unstemmed | Fibroblasts Mediate Ectopic Bone Formation of Calcium Phosphate Ceramics |
| title_short | Fibroblasts Mediate Ectopic Bone Formation of Calcium Phosphate Ceramics |
| title_sort | fibroblasts mediate ectopic bone formation of calcium phosphate ceramics |
| topic | bone regeneration calcium phosphate ceramics fibroblast |
| url | https://www.mdpi.com/1996-1944/15/7/2569 |
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