Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study
In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-07-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/18/7/8095 |
| _version_ | 1818256843908055040 |
|---|---|
| author | Wagner Vilegas Rosemeire C. L. R. Pietro Carol A. Munro Neil A. R. Gow Emerson Santos Keunsook Lee Kanya Preechasuth Juliana A. Severi Tatiana M. Souza-Moreira |
| author_facet | Wagner Vilegas Rosemeire C. L. R. Pietro Carol A. Munro Neil A. R. Gow Emerson Santos Keunsook Lee Kanya Preechasuth Juliana A. Severi Tatiana M. Souza-Moreira |
| author_sort | Wagner Vilegas |
| collection | DOAJ |
| description | In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level. |
| first_indexed | 2024-12-12T17:34:12Z |
| format | Article |
| id | doaj.art-a07153712bc74326877dce272a640959 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T17:34:12Z |
| publishDate | 2013-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a07153712bc74326877dce272a6409592022-12-22T00:17:16ZengMDPI AGMolecules1420-30492013-07-011878095810810.3390/molecules18078095Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided StudyWagner VilegasRosemeire C. L. R. PietroCarol A. MunroNeil A. R. GowEmerson SantosKeunsook LeeKanya PreechasuthJuliana A. SeveriTatiana M. Souza-MoreiraIn addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level.http://www.mdpi.com/1420-3049/18/7/8095Plinia cauliflora (Myrtaceae)ellagitanninCandidaantifungal activitycell wall |
| spellingShingle | Wagner Vilegas Rosemeire C. L. R. Pietro Carol A. Munro Neil A. R. Gow Emerson Santos Keunsook Lee Kanya Preechasuth Juliana A. Severi Tatiana M. Souza-Moreira Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study Molecules Plinia cauliflora (Myrtaceae) ellagitannin Candida antifungal activity cell wall |
| title | Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study |
| title_full | Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study |
| title_fullStr | Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study |
| title_full_unstemmed | Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study |
| title_short | Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study |
| title_sort | anti candida targets and cytotoxicity of casuarinin isolated from plinia cauliflora leaves in a bioactivity guided study |
| topic | Plinia cauliflora (Myrtaceae) ellagitannin Candida antifungal activity cell wall |
| url | http://www.mdpi.com/1420-3049/18/7/8095 |
| work_keys_str_mv | AT wagnervilegas anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT rosemeireclrpietro anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT carolamunro anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT neilargow anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT emersonsantos anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT keunsooklee anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT kanyapreechasuth anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT julianaaseveri anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT tatianamsouzamoreira anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy |