<i>Himatanthus bracteatus</i>-Composed In Situ Polymerizable Hydrogel for Wound Healing

<i>Himatanthus bracteatus</i>-Composed In Situ Polymerizable Hydrogel for Wound Healing

The <i>Himatanthus</i> genus presents anti-inflammatory, antioxidant activities, suggesting potential wound-healing properties. This study aimed to develop and analyze the wound-healing properties of a photopolymerizable gelatin-based hydrogel (GelMA) containing an ethanolic extract of &...

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Bibliographic Details
Main Authors: Bernadeth M. de Almeida, Izabella D. Dorta dos Santos, Felipe M. A. de Carvalho, Luana C. Correa, John L. S. Cunha, Claudio Dariva, Patricia Severino, Juliana C. Cardoso, Eliana B. Souto, Ricardo L. C. de Albuquerque-Júnior
Format: Article
Language:English
Published: MDPI AG 2022-12-01
Series:International Journal of Molecular Sciences
Subjects:
<i>Himatanthus bracteatus</i> ethanolic extract
gelatin methacryloyl hydrogel
GelMA
iridoids
Wistar rats
Online Access:https://www.mdpi.com/1422-0067/23/23/15176
Description
Summary:The <i>Himatanthus</i> genus presents anti-inflammatory, antioxidant activities, suggesting potential wound-healing properties. This study aimed to develop and analyze the wound-healing properties of a photopolymerizable gelatin-based hydrogel (GelMA) containing an ethanolic extract of <i>Himatanthus bracteatus</i> in a murine model. The extract was obtained under high pressure conditions, incorporated (2%) into the GelMA (GelMA-HB), and physically characterized. The anti-inflammatory activity of the extract was assessed using a carrageenan-induced pleurisy model and the GelMA-HB scarring properties in a wound-healing assay. The extract reduced IL-1β and TNF-α levels (48.5 ± 6.7 and 64.1 ± 4.9 pg/mL) compared to the vehicle (94.4 ± 2.3 pg/mL and 106.3 ± 5.7 pg/mL; <i>p</i> < 0.001). GelMA-HB depicted significantly lower swelling and increased resistance to mechanical compression compared to GelMA (<i>p</i> < 0.05). GelMA-HB accelerated wound closure over the time course of the experiment (<i>p</i> < 0.05) and promoted a significantly greater peak of myofibroblast differentiation (36.1 ± 6.6 cells) and microvascular density (23.1 ± 0.7 microvessels) on day 7 in comparison to GelMA (31.9 ± 5.3 cells and 20.2 ± 0.6 microvessels) and the control (25.8 ± 4.6 cells and 17.5 ± 0.5 microvessels) (<i>p</i> < 0.05). In conclusion, GelMA-HB improved wound healing in rodents, probably by modulating the inflammatory response and myofibroblastic and microvascular differentiation.
ISSN:1661-6596
1422-0067

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