<i>Himatanthus bracteatus</i>-Composed In Situ Polymerizable Hydrogel for Wound Healing
The <i>Himatanthus</i> genus presents anti-inflammatory, antioxidant activities, suggesting potential wound-healing properties. This study aimed to develop and analyze the wound-healing properties of a photopolymerizable gelatin-based hydrogel (GelMA) containing an ethanolic extract of &...
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/15176 |
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| author | Bernadeth M. de Almeida Izabella D. Dorta dos Santos Felipe M. A. de Carvalho Luana C. Correa John L. S. Cunha Claudio Dariva Patricia Severino Juliana C. Cardoso Eliana B. Souto Ricardo L. C. de Albuquerque-Júnior |
| author_facet | Bernadeth M. de Almeida Izabella D. Dorta dos Santos Felipe M. A. de Carvalho Luana C. Correa John L. S. Cunha Claudio Dariva Patricia Severino Juliana C. Cardoso Eliana B. Souto Ricardo L. C. de Albuquerque-Júnior |
| author_sort | Bernadeth M. de Almeida |
| collection | DOAJ |
| description | The <i>Himatanthus</i> genus presents anti-inflammatory, antioxidant activities, suggesting potential wound-healing properties. This study aimed to develop and analyze the wound-healing properties of a photopolymerizable gelatin-based hydrogel (GelMA) containing an ethanolic extract of <i>Himatanthus bracteatus</i> in a murine model. The extract was obtained under high pressure conditions, incorporated (2%) into the GelMA (GelMA-HB), and physically characterized. The anti-inflammatory activity of the extract was assessed using a carrageenan-induced pleurisy model and the GelMA-HB scarring properties in a wound-healing assay. The extract reduced IL-1β and TNF-α levels (48.5 ± 6.7 and 64.1 ± 4.9 pg/mL) compared to the vehicle (94.4 ± 2.3 pg/mL and 106.3 ± 5.7 pg/mL; <i>p</i> < 0.001). GelMA-HB depicted significantly lower swelling and increased resistance to mechanical compression compared to GelMA (<i>p</i> < 0.05). GelMA-HB accelerated wound closure over the time course of the experiment (<i>p</i> < 0.05) and promoted a significantly greater peak of myofibroblast differentiation (36.1 ± 6.6 cells) and microvascular density (23.1 ± 0.7 microvessels) on day 7 in comparison to GelMA (31.9 ± 5.3 cells and 20.2 ± 0.6 microvessels) and the control (25.8 ± 4.6 cells and 17.5 ± 0.5 microvessels) (<i>p</i> < 0.05). In conclusion, GelMA-HB improved wound healing in rodents, probably by modulating the inflammatory response and myofibroblastic and microvascular differentiation. |
| first_indexed | 2024-03-09T17:44:44Z |
| format | Article |
| id | doaj.art-a073d73d41d94d26985c9a65e38cdcd4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:44:44Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a073d73d41d94d26985c9a65e38cdcd42023-11-24T11:14:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123231517610.3390/ijms232315176<i>Himatanthus bracteatus</i>-Composed In Situ Polymerizable Hydrogel for Wound HealingBernadeth M. de Almeida0Izabella D. Dorta dos Santos1Felipe M. A. de Carvalho2Luana C. Correa3John L. S. Cunha4Claudio Dariva5Patricia Severino6Juliana C. Cardoso7Eliana B. Souto8Ricardo L. C. de Albuquerque-Júnior9Biotechnological Postgraduate Program—RENORBIO, Tiradentes University, Aracaju 49010-390, SE, BrazilPostgraduate Program in Health and Environment, Tiradentes University, Aracaju 49032-490, SE, BrazilPostgraduate Program in Health and Environment, Tiradentes University, Aracaju 49032-490, SE, BrazilSchool of Physiotherapy, Tiradentes University, Aracaju 49032-490, SE, BrazilDepartment of Odontology, Paraiba State University, Campina Grande 58429 500, PB, BrazilLaboratory for Colloidal Systems Studies, Institute of Technology and Research (ITP), Tiradentes University, Aracaju 49010-390, SE, BrazilBiotechnological Postgraduate Program—RENORBIO, Tiradentes University, Aracaju 49010-390, SE, BrazilBiotechnological Postgraduate Program—RENORBIO, Tiradentes University, Aracaju 49010-390, SE, BrazilBiotechnological Postgraduate Program—RENORBIO, Tiradentes University, Aracaju 49010-390, SE, BrazilDepartment of Pathology, Federal University of Santa Catarina, Florianópolis 88040-370, SC, BrazilThe <i>Himatanthus</i> genus presents anti-inflammatory, antioxidant activities, suggesting potential wound-healing properties. This study aimed to develop and analyze the wound-healing properties of a photopolymerizable gelatin-based hydrogel (GelMA) containing an ethanolic extract of <i>Himatanthus bracteatus</i> in a murine model. The extract was obtained under high pressure conditions, incorporated (2%) into the GelMA (GelMA-HB), and physically characterized. The anti-inflammatory activity of the extract was assessed using a carrageenan-induced pleurisy model and the GelMA-HB scarring properties in a wound-healing assay. The extract reduced IL-1β and TNF-α levels (48.5 ± 6.7 and 64.1 ± 4.9 pg/mL) compared to the vehicle (94.4 ± 2.3 pg/mL and 106.3 ± 5.7 pg/mL; <i>p</i> < 0.001). GelMA-HB depicted significantly lower swelling and increased resistance to mechanical compression compared to GelMA (<i>p</i> < 0.05). GelMA-HB accelerated wound closure over the time course of the experiment (<i>p</i> < 0.05) and promoted a significantly greater peak of myofibroblast differentiation (36.1 ± 6.6 cells) and microvascular density (23.1 ± 0.7 microvessels) on day 7 in comparison to GelMA (31.9 ± 5.3 cells and 20.2 ± 0.6 microvessels) and the control (25.8 ± 4.6 cells and 17.5 ± 0.5 microvessels) (<i>p</i> < 0.05). In conclusion, GelMA-HB improved wound healing in rodents, probably by modulating the inflammatory response and myofibroblastic and microvascular differentiation.https://www.mdpi.com/1422-0067/23/23/15176<i>Himatanthus bracteatus</i> ethanolic extractgelatin methacryloyl hydrogelGelMAiridoidsWistar rats |
| spellingShingle | Bernadeth M. de Almeida Izabella D. Dorta dos Santos Felipe M. A. de Carvalho Luana C. Correa John L. S. Cunha Claudio Dariva Patricia Severino Juliana C. Cardoso Eliana B. Souto Ricardo L. C. de Albuquerque-Júnior <i>Himatanthus bracteatus</i>-Composed In Situ Polymerizable Hydrogel for Wound Healing International Journal of Molecular Sciences <i>Himatanthus bracteatus</i> ethanolic extract gelatin methacryloyl hydrogel GelMA iridoids Wistar rats |
| title | <i>Himatanthus bracteatus</i>-Composed In Situ Polymerizable Hydrogel for Wound Healing |
| title_full | <i>Himatanthus bracteatus</i>-Composed In Situ Polymerizable Hydrogel for Wound Healing |
| title_fullStr | <i>Himatanthus bracteatus</i>-Composed In Situ Polymerizable Hydrogel for Wound Healing |
| title_full_unstemmed | <i>Himatanthus bracteatus</i>-Composed In Situ Polymerizable Hydrogel for Wound Healing |
| title_short | <i>Himatanthus bracteatus</i>-Composed In Situ Polymerizable Hydrogel for Wound Healing |
| title_sort | i himatanthus bracteatus i composed in situ polymerizable hydrogel for wound healing |
| topic | <i>Himatanthus bracteatus</i> ethanolic extract gelatin methacryloyl hydrogel GelMA iridoids Wistar rats |
| url | https://www.mdpi.com/1422-0067/23/23/15176 |
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