A protocol for a systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in diagnosis of invasive meningococcal disease in children
Abstract Background Meningococcal disease (MD) is notoriously difficult to diagnose in the early stages of the illness and presents similarly to many self-limiting viral infections. This mandates a cautious approach to diagnosis and initial management of suspected MD with many children receiving pre...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-06-01
|
| Series: | Systematic Reviews |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13643-018-0747-0 |
| _version_ | 1818991587027320832 |
|---|---|
| author | Thomas Waterfield Derek Fairley Fiona Lynn Bronagh Blackwood Michael D. Shields |
| author_facet | Thomas Waterfield Derek Fairley Fiona Lynn Bronagh Blackwood Michael D. Shields |
| author_sort | Thomas Waterfield |
| collection | DOAJ |
| description | Abstract Background Meningococcal disease (MD) is notoriously difficult to diagnose in the early stages of the illness and presents similarly to many self-limiting viral infections. This mandates a cautious approach to diagnosis and initial management of suspected MD with many children receiving precautionary broad-spectrum intravenous antibiotics. Despite this approach, some children are still diagnosed late. In the last 10 years, there have been advances in nucleic acid amplification techniques, and there is now a rapid test that can detect meningococcal DNA in under 30 min. This Loop-mediated-isothermal AMPlification (LAMP) technology may make it possible to diagnose MD at initial presentation thereby greatly improving outcomes and minimising harms through unnecessary treatment. The aim of this systematic review is to determine the diagnostic accuracy of LAMP technology in cases of suspected MD. The review has been registered with PROSPERO [CRD42017078026]. Methods To identify relevant studies, we will search MEDLINE, Embase, Web of Science, Scopus and The Cochrane Library. In additional, we will hand-search reference lists and grey literature including contacting the manufacturers of commercially available LAMP tests for MD for any unpublished data. Two reviewers will independently screen study eligibility and extract data. Methodological quality will be assessed, by two authors, according to the revised tool for the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2); any discrepancies will be resolved by a third author. The following test characteristics will be extracted into 2 × 2 tables for all included studies: true positives, false positives, true negatives, and false negatives. Study-specific estimates of sensitivity and specificity with 95% confidence intervals will be displayed in forest plots. To investigate heterogeneity, we will include covariates such as age, sample type, and study type into a bivariate random-effects model. Discussion This review will help determine the diagnostic accuracy of LAMP technology in diagnosing MD from blood, CSF and throat swabs in children. The data will help to define where in the diagnostic pathway LAMP could be useful including potential as a point-of-care test for children at first presentation. |
| first_indexed | 2024-12-20T20:12:38Z |
| format | Article |
| id | doaj.art-a075cf67051843b59598a5cec18f3788 |
| institution | Directory Open Access Journal |
| issn | 2046-4053 |
| language | English |
| last_indexed | 2024-12-20T20:12:38Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Systematic Reviews |
| spelling | doaj.art-a075cf67051843b59598a5cec18f37882022-12-21T19:27:47ZengBMCSystematic Reviews2046-40532018-06-01711510.1186/s13643-018-0747-0A protocol for a systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in diagnosis of invasive meningococcal disease in childrenThomas Waterfield0Derek Fairley1Fiona Lynn2Bronagh Blackwood3Michael D. Shields4Centre for Experimental Medicine, Wellcome Wolfson Institute of Experimental Medicine, Queen’s University BelfastBelfast Health and Social Care TrustCentre for Experimental Medicine, Wellcome Wolfson Institute of Experimental Medicine, Queen’s University BelfastCentre for Experimental Medicine, Wellcome Wolfson Institute of Experimental Medicine, Queen’s University BelfastCentre for Experimental Medicine, Wellcome Wolfson Institute of Experimental Medicine, Queen’s University BelfastAbstract Background Meningococcal disease (MD) is notoriously difficult to diagnose in the early stages of the illness and presents similarly to many self-limiting viral infections. This mandates a cautious approach to diagnosis and initial management of suspected MD with many children receiving precautionary broad-spectrum intravenous antibiotics. Despite this approach, some children are still diagnosed late. In the last 10 years, there have been advances in nucleic acid amplification techniques, and there is now a rapid test that can detect meningococcal DNA in under 30 min. This Loop-mediated-isothermal AMPlification (LAMP) technology may make it possible to diagnose MD at initial presentation thereby greatly improving outcomes and minimising harms through unnecessary treatment. The aim of this systematic review is to determine the diagnostic accuracy of LAMP technology in cases of suspected MD. The review has been registered with PROSPERO [CRD42017078026]. Methods To identify relevant studies, we will search MEDLINE, Embase, Web of Science, Scopus and The Cochrane Library. In additional, we will hand-search reference lists and grey literature including contacting the manufacturers of commercially available LAMP tests for MD for any unpublished data. Two reviewers will independently screen study eligibility and extract data. Methodological quality will be assessed, by two authors, according to the revised tool for the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2); any discrepancies will be resolved by a third author. The following test characteristics will be extracted into 2 × 2 tables for all included studies: true positives, false positives, true negatives, and false negatives. Study-specific estimates of sensitivity and specificity with 95% confidence intervals will be displayed in forest plots. To investigate heterogeneity, we will include covariates such as age, sample type, and study type into a bivariate random-effects model. Discussion This review will help determine the diagnostic accuracy of LAMP technology in diagnosing MD from blood, CSF and throat swabs in children. The data will help to define where in the diagnostic pathway LAMP could be useful including potential as a point-of-care test for children at first presentation.http://link.springer.com/article/10.1186/s13643-018-0747-0MeningococcalMeningitisSepsisNeisseria meningitidisTest accuracyMeta-analysis |
| spellingShingle | Thomas Waterfield Derek Fairley Fiona Lynn Bronagh Blackwood Michael D. Shields A protocol for a systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in diagnosis of invasive meningococcal disease in children Systematic Reviews Meningococcal Meningitis Sepsis Neisseria meningitidis Test accuracy Meta-analysis |
| title | A protocol for a systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in diagnosis of invasive meningococcal disease in children |
| title_full | A protocol for a systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in diagnosis of invasive meningococcal disease in children |
| title_fullStr | A protocol for a systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in diagnosis of invasive meningococcal disease in children |
| title_full_unstemmed | A protocol for a systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in diagnosis of invasive meningococcal disease in children |
| title_short | A protocol for a systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in diagnosis of invasive meningococcal disease in children |
| title_sort | protocol for a systematic review of the diagnostic accuracy of loop mediated isothermal amplification lamp in diagnosis of invasive meningococcal disease in children |
| topic | Meningococcal Meningitis Sepsis Neisseria meningitidis Test accuracy Meta-analysis |
| url | http://link.springer.com/article/10.1186/s13643-018-0747-0 |
| work_keys_str_mv | AT thomaswaterfield aprotocolforasystematicreviewofthediagnosticaccuracyofloopmediatedisothermalamplificationlampindiagnosisofinvasivemeningococcaldiseaseinchildren AT derekfairley aprotocolforasystematicreviewofthediagnosticaccuracyofloopmediatedisothermalamplificationlampindiagnosisofinvasivemeningococcaldiseaseinchildren AT fionalynn aprotocolforasystematicreviewofthediagnosticaccuracyofloopmediatedisothermalamplificationlampindiagnosisofinvasivemeningococcaldiseaseinchildren AT bronaghblackwood aprotocolforasystematicreviewofthediagnosticaccuracyofloopmediatedisothermalamplificationlampindiagnosisofinvasivemeningococcaldiseaseinchildren AT michaeldshields aprotocolforasystematicreviewofthediagnosticaccuracyofloopmediatedisothermalamplificationlampindiagnosisofinvasivemeningococcaldiseaseinchildren AT thomaswaterfield protocolforasystematicreviewofthediagnosticaccuracyofloopmediatedisothermalamplificationlampindiagnosisofinvasivemeningococcaldiseaseinchildren AT derekfairley protocolforasystematicreviewofthediagnosticaccuracyofloopmediatedisothermalamplificationlampindiagnosisofinvasivemeningococcaldiseaseinchildren AT fionalynn protocolforasystematicreviewofthediagnosticaccuracyofloopmediatedisothermalamplificationlampindiagnosisofinvasivemeningococcaldiseaseinchildren AT bronaghblackwood protocolforasystematicreviewofthediagnosticaccuracyofloopmediatedisothermalamplificationlampindiagnosisofinvasivemeningococcaldiseaseinchildren AT michaeldshields protocolforasystematicreviewofthediagnosticaccuracyofloopmediatedisothermalamplificationlampindiagnosisofinvasivemeningococcaldiseaseinchildren |