Role of Efflux in Antibiotic Resistance of Achromobacter xylosoxidans and Achromobacter insuavis Isolates From Patients With Cystic Fibrosis
Achromobacter genus (including Achromobacter xylosoxidans, the most prevalent Achromobacter species in patients with cystic fibrosis) is poorly susceptible to most conventional antibiotics. Contribution of efflux by AxyABM, AxyXY-OprZ, and AxyEF-OprN and of target mutations were studied in clinical...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-03-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.762307/full |
| _version_ | 1819178989993852928 |
|---|---|
| author | Hussein Chalhoub Stefanie Kampmeier Barbara C. Kahl Françoise Van Bambeke |
| author_facet | Hussein Chalhoub Stefanie Kampmeier Barbara C. Kahl Françoise Van Bambeke |
| author_sort | Hussein Chalhoub |
| collection | DOAJ |
| description | Achromobacter genus (including Achromobacter xylosoxidans, the most prevalent Achromobacter species in patients with cystic fibrosis) is poorly susceptible to most conventional antibiotics. Contribution of efflux by AxyABM, AxyXY-OprZ, and AxyEF-OprN and of target mutations were studied in clinical isolates of A. xylosoxidans and Achromobacter insuavis. Forty-one isolates longitudinally collected from 21 patients with CF were studied by whole-genome sequencing (WGS)-typing, determination of minimum inhibitory concentrations (MICs) of β-lactams, aminoglycosides, colistin, azithromycin, ciprofloxacin, chloramphenicol, and doxycycline, and expression (quantitative RT-PCR) and function (measure of the uptake of a fluorescent substrate) of efflux pumps. WGS-based typing resulted in 10 clusters comprising 2 or 3 isolates and 20 singletons. The efflux activity was high in strains with elevated MICs for amikacin or azithromycin. This work sheds a new light on the impact of efflux and target mutations in resistance of Achromobacter to several drugs. |
| first_indexed | 2024-12-22T21:51:19Z |
| format | Article |
| id | doaj.art-a077f2c6f1dc41a49facb775f8fea134 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T21:51:19Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a077f2c6f1dc41a49facb775f8fea1342022-12-21T18:11:21ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-03-011310.3389/fmicb.2022.762307762307Role of Efflux in Antibiotic Resistance of Achromobacter xylosoxidans and Achromobacter insuavis Isolates From Patients With Cystic FibrosisHussein Chalhoub0Stefanie Kampmeier1Barbara C. Kahl2Françoise Van Bambeke3Pharmacologie Cellulaire et Moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, BelgiumInstitute of Hygiene, University Hospital Münster, Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyPharmacologie Cellulaire et Moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, BelgiumAchromobacter genus (including Achromobacter xylosoxidans, the most prevalent Achromobacter species in patients with cystic fibrosis) is poorly susceptible to most conventional antibiotics. Contribution of efflux by AxyABM, AxyXY-OprZ, and AxyEF-OprN and of target mutations were studied in clinical isolates of A. xylosoxidans and Achromobacter insuavis. Forty-one isolates longitudinally collected from 21 patients with CF were studied by whole-genome sequencing (WGS)-typing, determination of minimum inhibitory concentrations (MICs) of β-lactams, aminoglycosides, colistin, azithromycin, ciprofloxacin, chloramphenicol, and doxycycline, and expression (quantitative RT-PCR) and function (measure of the uptake of a fluorescent substrate) of efflux pumps. WGS-based typing resulted in 10 clusters comprising 2 or 3 isolates and 20 singletons. The efflux activity was high in strains with elevated MICs for amikacin or azithromycin. This work sheds a new light on the impact of efflux and target mutations in resistance of Achromobacter to several drugs.https://www.frontiersin.org/articles/10.3389/fmicb.2022.762307/fullAchromobactereffluxtarget mutationmacrolidefluoroquinoloneaminoglycoside |
| spellingShingle | Hussein Chalhoub Stefanie Kampmeier Barbara C. Kahl Françoise Van Bambeke Role of Efflux in Antibiotic Resistance of Achromobacter xylosoxidans and Achromobacter insuavis Isolates From Patients With Cystic Fibrosis Frontiers in Microbiology Achromobacter efflux target mutation macrolide fluoroquinolone aminoglycoside |
| title | Role of Efflux in Antibiotic Resistance of Achromobacter xylosoxidans and Achromobacter insuavis Isolates From Patients With Cystic Fibrosis |
| title_full | Role of Efflux in Antibiotic Resistance of Achromobacter xylosoxidans and Achromobacter insuavis Isolates From Patients With Cystic Fibrosis |
| title_fullStr | Role of Efflux in Antibiotic Resistance of Achromobacter xylosoxidans and Achromobacter insuavis Isolates From Patients With Cystic Fibrosis |
| title_full_unstemmed | Role of Efflux in Antibiotic Resistance of Achromobacter xylosoxidans and Achromobacter insuavis Isolates From Patients With Cystic Fibrosis |
| title_short | Role of Efflux in Antibiotic Resistance of Achromobacter xylosoxidans and Achromobacter insuavis Isolates From Patients With Cystic Fibrosis |
| title_sort | role of efflux in antibiotic resistance of achromobacter xylosoxidans and achromobacter insuavis isolates from patients with cystic fibrosis |
| topic | Achromobacter efflux target mutation macrolide fluoroquinolone aminoglycoside |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.762307/full |
| work_keys_str_mv | AT husseinchalhoub roleofeffluxinantibioticresistanceofachromobacterxylosoxidansandachromobacterinsuavisisolatesfrompatientswithcysticfibrosis AT stefaniekampmeier roleofeffluxinantibioticresistanceofachromobacterxylosoxidansandachromobacterinsuavisisolatesfrompatientswithcysticfibrosis AT barbarackahl roleofeffluxinantibioticresistanceofachromobacterxylosoxidansandachromobacterinsuavisisolatesfrompatientswithcysticfibrosis AT francoisevanbambeke roleofeffluxinantibioticresistanceofachromobacterxylosoxidansandachromobacterinsuavisisolatesfrompatientswithcysticfibrosis |