Effect of nitroprusside on furosemide-induced skeletal teratogenicity in rat fetuses
Background: Furosemide as a loop diuretic can use in treatment of hypertension, renal or heart failures and cirrhosis, when sodium retention is significant. It is known that use of furosemide can be lead congenital abnormalities in humans and animals. Nitroprusside as a NO donor can decrease blood s...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bushehr University of Medical Sciences
2016-01-01
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| Series: | Iranian South Medical Journal |
| Subjects: | |
| Online Access: | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-656&slc_lang=en&sid=1 |
| _version_ | 1818483950377500672 |
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| author | Mahmood Khaksary Mahabady Hosean Najafzadeh Varzi Mozhgan Sabet |
| author_facet | Mahmood Khaksary Mahabady Hosean Najafzadeh Varzi Mozhgan Sabet |
| author_sort | Mahmood Khaksary Mahabady |
| collection | DOAJ |
| description | Background: Furosemide as a loop diuretic can use in treatment of hypertension, renal or heart failures and cirrhosis, when sodium retention is significant. It is known that use of furosemide can be lead congenital abnormalities in humans and animals. Nitroprusside as a NO donor can decrease blood supply complications and constriction of placenta and uterus via vasodilation and improvment blood supply. The aim of this study was preventation or decrease of teratogenicity form furosemide in rat fetuses by sodium nitroprusside.
Materials and Methods: This study was performed on 28 pregnant rats that were divided into four groups, the groups consist control, furosemide, sodium nitroprusside and furosemide plus sodium nitroprusside. Drugs were administrated on 14th and 16th day of gestation. Test groups received furosemide (200mg/kg) orally, and nitroprusside (0.5 mg/kg) intraperitoneally. The rats were euthanized and fetuses were collected at 19th day of gestation, after weight and length determination, they stained by Alizarin red- Alician blue method. Then the skeletal system of the stained fetuses was investigated by stereomicroscope for teratogenicity effects.
Results: The results showed the cleft palate, wavy ribs and decreased ossification mean incidence in forelimbs and hindlimbs were 11.11%, 68.88% and 20% in the fetuses of the rats received furosemide, where as it decreased to 7.31%, 21.95% and 12.19% in group which received furosemide plus nitroprusside, respectively.
Conclusion: It is concluded that sodium nitroprusside can significantly decrease teratogenicity induced by furosemide. |
| first_indexed | 2024-12-10T15:48:46Z |
| format | Article |
| id | doaj.art-a0783d6acf9d4ad0ba60413f750f29c3 |
| institution | Directory Open Access Journal |
| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-12-10T15:48:46Z |
| publishDate | 2016-01-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-a0783d6acf9d4ad0ba60413f750f29c32022-12-22T01:42:52ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542016-01-0118611491163Effect of nitroprusside on furosemide-induced skeletal teratogenicity in rat fetusesMahmood Khaksary Mahabady0Hosean Najafzadeh Varzi1Mozhgan Sabet2 Department of Anatomy and Embryology, Faculty of Veterinary Medicine, ShahidChamran University, Ahvaz, Iran Department of Pharmacology, Faculty of Veterinary Medicine, ShahidChamran University, Ahvaz, Iran Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran Background: Furosemide as a loop diuretic can use in treatment of hypertension, renal or heart failures and cirrhosis, when sodium retention is significant. It is known that use of furosemide can be lead congenital abnormalities in humans and animals. Nitroprusside as a NO donor can decrease blood supply complications and constriction of placenta and uterus via vasodilation and improvment blood supply. The aim of this study was preventation or decrease of teratogenicity form furosemide in rat fetuses by sodium nitroprusside. Materials and Methods: This study was performed on 28 pregnant rats that were divided into four groups, the groups consist control, furosemide, sodium nitroprusside and furosemide plus sodium nitroprusside. Drugs were administrated on 14th and 16th day of gestation. Test groups received furosemide (200mg/kg) orally, and nitroprusside (0.5 mg/kg) intraperitoneally. The rats were euthanized and fetuses were collected at 19th day of gestation, after weight and length determination, they stained by Alizarin red- Alician blue method. Then the skeletal system of the stained fetuses was investigated by stereomicroscope for teratogenicity effects. Results: The results showed the cleft palate, wavy ribs and decreased ossification mean incidence in forelimbs and hindlimbs were 11.11%, 68.88% and 20% in the fetuses of the rats received furosemide, where as it decreased to 7.31%, 21.95% and 12.19% in group which received furosemide plus nitroprusside, respectively. Conclusion: It is concluded that sodium nitroprusside can significantly decrease teratogenicity induced by furosemide.http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-656&slc_lang=en&sid=1Teratogenicity Furosemide Nitroprusside Rat Skeletal system |
| spellingShingle | Mahmood Khaksary Mahabady Hosean Najafzadeh Varzi Mozhgan Sabet Effect of nitroprusside on furosemide-induced skeletal teratogenicity in rat fetuses Iranian South Medical Journal Teratogenicity Furosemide Nitroprusside Rat Skeletal system |
| title | Effect of nitroprusside on furosemide-induced skeletal teratogenicity in rat fetuses |
| title_full | Effect of nitroprusside on furosemide-induced skeletal teratogenicity in rat fetuses |
| title_fullStr | Effect of nitroprusside on furosemide-induced skeletal teratogenicity in rat fetuses |
| title_full_unstemmed | Effect of nitroprusside on furosemide-induced skeletal teratogenicity in rat fetuses |
| title_short | Effect of nitroprusside on furosemide-induced skeletal teratogenicity in rat fetuses |
| title_sort | effect of nitroprusside on furosemide induced skeletal teratogenicity in rat fetuses |
| topic | Teratogenicity Furosemide Nitroprusside Rat Skeletal system |
| url | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-656&slc_lang=en&sid=1 |
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