GENETIC POLYMORPHISM AT THE Β-LACTOGLOBULIN LOCUS IN A DAIRY HERD OF ROMANIAN SPOTTED AND BROWN OF MARAMURES BREEDS
The objective of the present study was focused on possibilities to estimate the allele and genotype frequencies of β-lactoglobulin (BLG) gene polymorphisms in dairy cattle belonging to two different genetic groups from the Research and Development Station for Bovine Raising Arad in order to have bre...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-11-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1695 |
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| author | DANIELA ILIE AURELIA SĂLĂJEANU ANUŢA MAGDIN CLAUDIA STANCA I. VINTILĂ |
| author_facet | DANIELA ILIE AURELIA SĂLĂJEANU ANUŢA MAGDIN CLAUDIA STANCA I. VINTILĂ |
| author_sort | DANIELA ILIE |
| collection | DOAJ |
| description | The objective of the present study was focused on possibilities to estimate the allele
and genotype frequencies of β-lactoglobulin (BLG) gene polymorphisms in dairy
cattle belonging to two different genetic groups from the Research and Development
Station for Bovine Raising Arad in order to have breeding programs that target an
increase in the frequency of the B allele in the dairy cattle population. Genotyping
was performed on 20 Romanian Spotted and 18 Brown of Maramures cattle.In order
to differentiate the favorable genotype for superior composition and higher cheese
yield, we used simple DNA extraction method from fresh blood and techniques based
on DNA analysis, which include polymerase chain reaction and restriction fragment
length polymorphisms (PCR-RFLP) methods. Employing these techniques we were
able to determine the (BLG) genotype of all individuals in a given population under
selection, regardless of sex, age or physiological stage. As a result, it is now possible
to include information on milk protein genotypes into marker assisted selection
programs and consequently improve response to selection. |
| first_indexed | 2024-04-24T11:48:14Z |
| format | Article |
| id | doaj.art-a07925c034bd4a9ea4c3f97c5562dd31 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:48:14Z |
| publishDate | 2023-11-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-a07925c034bd4a9ea4c3f97c5562dd312024-04-09T08:53:17ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-11-014111041041695GENETIC POLYMORPHISM AT THE Β-LACTOGLOBULIN LOCUS IN A DAIRY HERD OF ROMANIAN SPOTTED AND BROWN OF MARAMURES BREEDSDANIELA ILIE0AURELIA SĂLĂJEANU1ANUŢA MAGDIN2CLAUDIA STANCA3I. VINTILĂ4Research and Development Station for Bovine Raising - Arad Research and Development Station for Bovine Raising - Arad Research and Development Station for Bovine Raising - Arad Faculty of Animal Science and Biotechnology, Timişoara, România Research and Development Station for Bovine Raising - Arad The objective of the present study was focused on possibilities to estimate the allele and genotype frequencies of β-lactoglobulin (BLG) gene polymorphisms in dairy cattle belonging to two different genetic groups from the Research and Development Station for Bovine Raising Arad in order to have breeding programs that target an increase in the frequency of the B allele in the dairy cattle population. Genotyping was performed on 20 Romanian Spotted and 18 Brown of Maramures cattle.In order to differentiate the favorable genotype for superior composition and higher cheese yield, we used simple DNA extraction method from fresh blood and techniques based on DNA analysis, which include polymerase chain reaction and restriction fragment length polymorphisms (PCR-RFLP) methods. Employing these techniques we were able to determine the (BLG) genotype of all individuals in a given population under selection, regardless of sex, age or physiological stage. As a result, it is now possible to include information on milk protein genotypes into marker assisted selection programs and consequently improve response to selection.https://spasb.ro/index.php/public_html/article/view/1695β-lactoglobulinpcr-rflp analysesdna genotype |
| spellingShingle | DANIELA ILIE AURELIA SĂLĂJEANU ANUŢA MAGDIN CLAUDIA STANCA I. VINTILĂ GENETIC POLYMORPHISM AT THE Β-LACTOGLOBULIN LOCUS IN A DAIRY HERD OF ROMANIAN SPOTTED AND BROWN OF MARAMURES BREEDS Scientific Papers Animal Science and Biotechnologies β-lactoglobulin pcr-rflp analyses dna genotype |
| title | GENETIC POLYMORPHISM AT THE Β-LACTOGLOBULIN LOCUS IN A DAIRY HERD OF ROMANIAN SPOTTED AND BROWN OF MARAMURES BREEDS |
| title_full | GENETIC POLYMORPHISM AT THE Β-LACTOGLOBULIN LOCUS IN A DAIRY HERD OF ROMANIAN SPOTTED AND BROWN OF MARAMURES BREEDS |
| title_fullStr | GENETIC POLYMORPHISM AT THE Β-LACTOGLOBULIN LOCUS IN A DAIRY HERD OF ROMANIAN SPOTTED AND BROWN OF MARAMURES BREEDS |
| title_full_unstemmed | GENETIC POLYMORPHISM AT THE Β-LACTOGLOBULIN LOCUS IN A DAIRY HERD OF ROMANIAN SPOTTED AND BROWN OF MARAMURES BREEDS |
| title_short | GENETIC POLYMORPHISM AT THE Β-LACTOGLOBULIN LOCUS IN A DAIRY HERD OF ROMANIAN SPOTTED AND BROWN OF MARAMURES BREEDS |
| title_sort | genetic polymorphism at the β lactoglobulin locus in a dairy herd of romanian spotted and brown of maramures breeds |
| topic | β-lactoglobulin pcr-rflp analyses dna genotype |
| url | https://spasb.ro/index.php/public_html/article/view/1695 |
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