<b>Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from <i>Halymenia</i> sp. (Rhodophyceae)</b> - doi: 10.4025/actascibiolsci.v34i1.6961
Sulfated polysaccharides (SPs) have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II) extracted from <em>Halymenia</em> sp Rhodophyceae in order to compare the yield, purification and anticoagulant...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2011-11-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6961 |
| _version_ | 1818245529169035264 |
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| author | Norma Maria Barros Benevides Érika Façanha Bessa Ismael Nilo Lino de Queiroz Ana Luíza Gomes Quinderé Edfranck de Sousa Oliveira Vanderlei José Ariévilo Gurgel Rodrigues |
| author_facet | Norma Maria Barros Benevides Érika Façanha Bessa Ismael Nilo Lino de Queiroz Ana Luíza Gomes Quinderé Edfranck de Sousa Oliveira Vanderlei José Ariévilo Gurgel Rodrigues |
| author_sort | Norma Maria Barros Benevides |
| collection | DOAJ |
| description | Sulfated polysaccharides (SPs) have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II) extracted from <em>Halymenia</em> sp Rhodophyceae in order to compare the yield, purification and anticoagulant activity. The total SPs (TSPs) were first extracted with papain in 100 mM sodium acetate (pH 5.0) containing cysteine and EDTA (5 mM). The TSPs obtained were dried in an oven (M I) or lyophilized (M II) and then examined by ion exchange chromatography (DEAE-cellulose) using the NaCl gradient technique. The fractions were analyzed by 0.5% agarose gel electrophoresis and the <em>in vitro</em> anticoagulant activity was evaluated by the activated partial thromboplastin time test using normal human plasma and compared to heparin (HEP) (193.00 IU mg<sup>-1</sup>). There was a difference in TSP yield of 19.05% and similar chromatographic SP profiles. Electrophoresis revealed fractions with distinct resolutions. The fractions eluted with 0.75 M of salt (M I and II) were the most active, measuring 27.40 and 72.66 IU mg<sup>-1</sup>, respectively, when compared to HEP. Therefore, obtaining SP with anticoagulant activity from <em>Halymenia</em> sp. is more efficient by freeze-drying.<p> </p> |
| first_indexed | 2024-12-12T14:34:22Z |
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| id | doaj.art-a07b600631ae4298b33180ea144baa99 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-12-12T14:34:22Z |
| publishDate | 2011-11-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-a07b600631ae4298b33180ea144baa992022-12-22T00:21:26ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2011-11-01341511<b>Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from <i>Halymenia</i> sp. (Rhodophyceae)</b> - doi: 10.4025/actascibiolsci.v34i1.6961Norma Maria Barros BenevidesÉrika Façanha BessaIsmael Nilo Lino de QueirozAna Luíza Gomes QuinderéEdfranck de Sousa Oliveira VanderleiJosé Ariévilo Gurgel RodriguesSulfated polysaccharides (SPs) have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II) extracted from <em>Halymenia</em> sp Rhodophyceae in order to compare the yield, purification and anticoagulant activity. The total SPs (TSPs) were first extracted with papain in 100 mM sodium acetate (pH 5.0) containing cysteine and EDTA (5 mM). The TSPs obtained were dried in an oven (M I) or lyophilized (M II) and then examined by ion exchange chromatography (DEAE-cellulose) using the NaCl gradient technique. The fractions were analyzed by 0.5% agarose gel electrophoresis and the <em>in vitro</em> anticoagulant activity was evaluated by the activated partial thromboplastin time test using normal human plasma and compared to heparin (HEP) (193.00 IU mg<sup>-1</sup>). There was a difference in TSP yield of 19.05% and similar chromatographic SP profiles. Electrophoresis revealed fractions with distinct resolutions. The fractions eluted with 0.75 M of salt (M I and II) were the most active, measuring 27.40 and 72.66 IU mg<sup>-1</sup>, respectively, when compared to HEP. Therefore, obtaining SP with anticoagulant activity from <em>Halymenia</em> sp. is more efficient by freeze-drying.<p> </p>http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6961Halymenialessulfated macromoleculesdrying methodsblood coagulation |
| spellingShingle | Norma Maria Barros Benevides Érika Façanha Bessa Ismael Nilo Lino de Queiroz Ana Luíza Gomes Quinderé Edfranck de Sousa Oliveira Vanderlei José Ariévilo Gurgel Rodrigues <b>Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from <i>Halymenia</i> sp. (Rhodophyceae)</b> - doi: 10.4025/actascibiolsci.v34i1.6961 Acta Scientiarum: Biological Sciences Halymeniales sulfated macromolecules drying methods blood coagulation |
| title | <b>Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from <i>Halymenia</i> sp. (Rhodophyceae)</b> - doi: 10.4025/actascibiolsci.v34i1.6961 |
| title_full | <b>Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from <i>Halymenia</i> sp. (Rhodophyceae)</b> - doi: 10.4025/actascibiolsci.v34i1.6961 |
| title_fullStr | <b>Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from <i>Halymenia</i> sp. (Rhodophyceae)</b> - doi: 10.4025/actascibiolsci.v34i1.6961 |
| title_full_unstemmed | <b>Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from <i>Halymenia</i> sp. (Rhodophyceae)</b> - doi: 10.4025/actascibiolsci.v34i1.6961 |
| title_short | <b>Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from <i>Halymenia</i> sp. (Rhodophyceae)</b> - doi: 10.4025/actascibiolsci.v34i1.6961 |
| title_sort | b analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from i halymenia i sp rhodophyceae b doi 10 4025 actascibiolsci v34i1 6961 |
| topic | Halymeniales sulfated macromolecules drying methods blood coagulation |
| url | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6961 |
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