<em>Scytosiphon lomentaria</em> Extract Ameliorates Obesity and Modulates Gut Microbiota in High-Fat-Diet-Fed Mice
<i>Scytosiphon lomentaria</i> (SL) is a brown seaweed with antioxidant and anti-inflammatory properties; however, its effects on obesity are unknown. In this research, we investigated the anti-obesity properties and underlying mechanisms of the SL extract in vitro and in vivo. In 3T3-L1...
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MDPI AG
2023-02-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/4/815 |
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| author | Jing Yan Jinwoo Bak Yula Go Jumin Park Minkyoung Park Hae-Jeung Lee Hyemee Kim |
| author_facet | Jing Yan Jinwoo Bak Yula Go Jumin Park Minkyoung Park Hae-Jeung Lee Hyemee Kim |
| author_sort | Jing Yan |
| collection | DOAJ |
| description | <i>Scytosiphon lomentaria</i> (SL) is a brown seaweed with antioxidant and anti-inflammatory properties; however, its effects on obesity are unknown. In this research, we investigated the anti-obesity properties and underlying mechanisms of the SL extract in vitro and in vivo. In 3T3-L1 preadipocytes, SL extract inhibited lipid accumulation, decreased the expression of <i>Acc1</i>, <i>C/ebpa</i>, <i>Pparg</i> mRNA and p-ACC1, and increased the expression of <i>Ucp1</i> mRNA, UCP1 and p-AMPK. In animal experiments, mice were fed a chow diet, a high-fat diet (HF; 60% of calories as fat), and high-fat diet with SL extract (150 and 300 mg/kg body weight) for eight weeks (<i>n</i> = 10/group). SL extract reduced HF-induced weight gain, epididymal fat weight, fat cell size, LDL-C, leptin, fasting glucose, and glucose tolerance. In addition, SL extract had comparable effects on mRNA expression in WAT and liver to those observed in vitro, thereby inhibiting p-ACC1/ACC1 and increasing p-AMPK/AMPK and UCP1 expression. Furthermore, SL extract decreased HF-induced Firmicutes/Bacteroidetes ratio and reversed HF-reduced <i>Bacteroides</i> spp., <i>Bacteroides vulgatus</i>, and <i>Faecalibacterium prausnitzii</i>. These findings suggest that SL extract can aid in weight loss in mice fed a high-fat diet by altering adipogenic and thermogenic pathways, as well as gut microbiota composition. |
| first_indexed | 2024-03-11T08:20:06Z |
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| language | English |
| last_indexed | 2024-03-11T08:20:06Z |
| publishDate | 2023-02-01 |
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| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-a07f469e427a4bd6a2d1083f2dd7509d2023-11-16T22:29:22ZengMDPI AGNutrients2072-66432023-02-0115481510.3390/nu15040815<em>Scytosiphon lomentaria</em> Extract Ameliorates Obesity and Modulates Gut Microbiota in High-Fat-Diet-Fed MiceJing Yan0Jinwoo Bak1Yula Go2Jumin Park3Minkyoung Park4Hae-Jeung Lee5Hyemee Kim6Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of KoreaDepartment of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of KoreaDepartment of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of KoreaDepartment of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of KoreaDepartment of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of KoreaDepartment of Food and Nutrition, Gachon University, Seongnam-si 13120, Republic of KoreaDepartment of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of Korea<i>Scytosiphon lomentaria</i> (SL) is a brown seaweed with antioxidant and anti-inflammatory properties; however, its effects on obesity are unknown. In this research, we investigated the anti-obesity properties and underlying mechanisms of the SL extract in vitro and in vivo. In 3T3-L1 preadipocytes, SL extract inhibited lipid accumulation, decreased the expression of <i>Acc1</i>, <i>C/ebpa</i>, <i>Pparg</i> mRNA and p-ACC1, and increased the expression of <i>Ucp1</i> mRNA, UCP1 and p-AMPK. In animal experiments, mice were fed a chow diet, a high-fat diet (HF; 60% of calories as fat), and high-fat diet with SL extract (150 and 300 mg/kg body weight) for eight weeks (<i>n</i> = 10/group). SL extract reduced HF-induced weight gain, epididymal fat weight, fat cell size, LDL-C, leptin, fasting glucose, and glucose tolerance. In addition, SL extract had comparable effects on mRNA expression in WAT and liver to those observed in vitro, thereby inhibiting p-ACC1/ACC1 and increasing p-AMPK/AMPK and UCP1 expression. Furthermore, SL extract decreased HF-induced Firmicutes/Bacteroidetes ratio and reversed HF-reduced <i>Bacteroides</i> spp., <i>Bacteroides vulgatus</i>, and <i>Faecalibacterium prausnitzii</i>. These findings suggest that SL extract can aid in weight loss in mice fed a high-fat diet by altering adipogenic and thermogenic pathways, as well as gut microbiota composition.https://www.mdpi.com/2072-6643/15/4/815<em>Scytosiphon lomentaria</em>miceanti-obesitygut microbiota |
| spellingShingle | Jing Yan Jinwoo Bak Yula Go Jumin Park Minkyoung Park Hae-Jeung Lee Hyemee Kim <em>Scytosiphon lomentaria</em> Extract Ameliorates Obesity and Modulates Gut Microbiota in High-Fat-Diet-Fed Mice Nutrients <em>Scytosiphon lomentaria</em> mice anti-obesity gut microbiota |
| title | <em>Scytosiphon lomentaria</em> Extract Ameliorates Obesity and Modulates Gut Microbiota in High-Fat-Diet-Fed Mice |
| title_full | <em>Scytosiphon lomentaria</em> Extract Ameliorates Obesity and Modulates Gut Microbiota in High-Fat-Diet-Fed Mice |
| title_fullStr | <em>Scytosiphon lomentaria</em> Extract Ameliorates Obesity and Modulates Gut Microbiota in High-Fat-Diet-Fed Mice |
| title_full_unstemmed | <em>Scytosiphon lomentaria</em> Extract Ameliorates Obesity and Modulates Gut Microbiota in High-Fat-Diet-Fed Mice |
| title_short | <em>Scytosiphon lomentaria</em> Extract Ameliorates Obesity and Modulates Gut Microbiota in High-Fat-Diet-Fed Mice |
| title_sort | em scytosiphon lomentaria em extract ameliorates obesity and modulates gut microbiota in high fat diet fed mice |
| topic | <em>Scytosiphon lomentaria</em> mice anti-obesity gut microbiota |
| url | https://www.mdpi.com/2072-6643/15/4/815 |
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