CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae
Abstract Background Genome editing tools are important for functional genomics research and biotechnology applications. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 (Cas9) system for gene knockout has emerged as the most effective genom...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-08-01
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| Series: | Rice |
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| Online Access: | http://link.springer.com/article/10.1186/s12284-019-0325-7 |
| _version_ | 1818250084737875968 |
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| author | Young-Ah Kim Hyeran Moon Chang-Jin Park |
| author_facet | Young-Ah Kim Hyeran Moon Chang-Jin Park |
| author_sort | Young-Ah Kim |
| collection | DOAJ |
| description | Abstract Background Genome editing tools are important for functional genomics research and biotechnology applications. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 (Cas9) system for gene knockout has emerged as the most effective genome-editing tool. It has previously been reported that, in rice plants, knockdown of the Os8N3 gene resulted in enhanced resistance to Xanthomonas oryzae pv. oryzae (Xoo), while displaying abnormal pollen development. Results The CRISPR/Cas9 system was employed to knockout rice Os8N3, in order to confer enhanced resistance to Xoo. Analysis of the genotypes and edited Os8N3 in T0, T1, T2, and T3 transgenic rice plants showed that the mutations were transmitted to subsequent generations, and homozygous mutants displayed significantly enhanced resistance to Xoo. Stable transmission of CRISPR/Cas9-mediated Os8N3 gene editing without the transferred DNA (T-DNA) was confirmed by segregation in the T1 generation. With respect to many investigated agronomic traits including pollen development, there was no significant difference between homozygous mutants and non-transgenic control plants under greenhouse growth conditions. Conclusion Data from this study indicate that the CRISPR/Cas9-mediated Os8N3 edition can be successfully employed for non-transgenic crop improvements. |
| first_indexed | 2024-12-12T15:46:46Z |
| format | Article |
| id | doaj.art-a07f985c0a074f26a890fac1ab4626fb |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-12-12T15:46:46Z |
| publishDate | 2019-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-a07f985c0a074f26a890fac1ab4626fb2022-12-22T00:19:44ZengSpringerOpenRice1939-84251939-84332019-08-0112111310.1186/s12284-019-0325-7CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzaeYoung-Ah Kim0Hyeran Moon1Chang-Jin Park2Department of Plant Biotechnology, Sejong UniversityDepartment of Molecular Biology, Sejong UniversityDepartment of Plant Biotechnology, Sejong UniversityAbstract Background Genome editing tools are important for functional genomics research and biotechnology applications. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 (Cas9) system for gene knockout has emerged as the most effective genome-editing tool. It has previously been reported that, in rice plants, knockdown of the Os8N3 gene resulted in enhanced resistance to Xanthomonas oryzae pv. oryzae (Xoo), while displaying abnormal pollen development. Results The CRISPR/Cas9 system was employed to knockout rice Os8N3, in order to confer enhanced resistance to Xoo. Analysis of the genotypes and edited Os8N3 in T0, T1, T2, and T3 transgenic rice plants showed that the mutations were transmitted to subsequent generations, and homozygous mutants displayed significantly enhanced resistance to Xoo. Stable transmission of CRISPR/Cas9-mediated Os8N3 gene editing without the transferred DNA (T-DNA) was confirmed by segregation in the T1 generation. With respect to many investigated agronomic traits including pollen development, there was no significant difference between homozygous mutants and non-transgenic control plants under greenhouse growth conditions. Conclusion Data from this study indicate that the CRISPR/Cas9-mediated Os8N3 edition can be successfully employed for non-transgenic crop improvements.http://link.springer.com/article/10.1186/s12284-019-0325-7CRISPR/Cas9Disease resistanceOs8N3Ricexa13Xanthomonas oryzae pv. oryzae |
| spellingShingle | Young-Ah Kim Hyeran Moon Chang-Jin Park CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae Rice CRISPR/Cas9 Disease resistance Os8N3 Rice xa13 Xanthomonas oryzae pv. oryzae |
| title | CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae |
| title_full | CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae |
| title_fullStr | CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae |
| title_full_unstemmed | CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae |
| title_short | CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae |
| title_sort | crispr cas9 targeted mutagenesis of os8n3 in rice to confer resistance to xanthomonas oryzae pv oryzae |
| topic | CRISPR/Cas9 Disease resistance Os8N3 Rice xa13 Xanthomonas oryzae pv. oryzae |
| url | http://link.springer.com/article/10.1186/s12284-019-0325-7 |
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