Plasma Bacterial DNA Load as a Potential Biomarker for the Early Detection of Colorectal Cancer: A Case–Control Study
The gut microbiota has gained increasing attention in recent years due to its significant impact on colorectal cancer (CRC) development and progression. The recent detection of bacterial DNA load in plasma holds promise as a potential non-invasive approach for early cancer detection. The aim of this...
| Main Authors: | , , , , , , , , , , , , , , |
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MDPI AG
2023-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/9/2360 |
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| author | Robertina Giacconi Rossella Donghia Graziana Arborea Maria Teresa Savino Mauro Provinciali Fabrizia Lattanzio Giusy Rita Caponio Sergio Coletta Antonia Bianco Maria Notarnicola Caterina Bonfiglio Giuseppe Passarino Patrizia D’Aquila Dina Bellizzi Pasqua Letizia Pesole |
| author_facet | Robertina Giacconi Rossella Donghia Graziana Arborea Maria Teresa Savino Mauro Provinciali Fabrizia Lattanzio Giusy Rita Caponio Sergio Coletta Antonia Bianco Maria Notarnicola Caterina Bonfiglio Giuseppe Passarino Patrizia D’Aquila Dina Bellizzi Pasqua Letizia Pesole |
| author_sort | Robertina Giacconi |
| collection | DOAJ |
| description | The gut microbiota has gained increasing attention in recent years due to its significant impact on colorectal cancer (CRC) development and progression. The recent detection of bacterial DNA load in plasma holds promise as a potential non-invasive approach for early cancer detection. The aim of this study was to examine the quantity of bacterial DNA present in the plasma of 50 patients who have CRC in comparison to 40 neoplastic disease-free patients, as well as to determine if there is a correlation between the amount of plasma bacterial DNA and various clinical parameters. Plasma bacterial DNA levels were found to be elevated in the CRC group compared to the control group. As it emerged from the logistic analysis (adjusted for age and gender), these levels were strongly associated with the risk of CRC (OR = 1.02, <i>p</i> < 0.001, 95% C.I.: 1.01–1.03). Moreover, an association was identified between a reduction in tumor mass and the highest tertile of plasma bacterial DNA. Our findings indicate that individuals with CRC displayed a higher plasma bacterial DNA load compared to healthy controls. This observation lends support to the theory of heightened bacterial migration from the gastrointestinal tract to the bloodstream in CRC. Furthermore, our results establish a link between this phenomenon and the size of the tumor mass. |
| first_indexed | 2024-03-10T22:26:20Z |
| format | Article |
| id | doaj.art-a0802fe973444c6fb8ae38f3f0af98b4 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T22:26:20Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a0802fe973444c6fb8ae38f3f0af98b42023-11-19T12:04:17ZengMDPI AGMicroorganisms2076-26072023-09-01119236010.3390/microorganisms11092360Plasma Bacterial DNA Load as a Potential Biomarker for the Early Detection of Colorectal Cancer: A Case–Control StudyRobertina Giacconi0Rossella Donghia1Graziana Arborea2Maria Teresa Savino3Mauro Provinciali4Fabrizia Lattanzio5Giusy Rita Caponio6Sergio Coletta7Antonia Bianco8Maria Notarnicola9Caterina Bonfiglio10Giuseppe Passarino11Patrizia D’Aquila12Dina Bellizzi13Pasqua Letizia Pesole14Advanced Technology Center for Aging Research, IRCCS INRCA, Via Birarelli 8, 60121 Ancona, ItalyNational Institute of Gastroenterology—IRCCS “Saverio de Bellis”, 70013 Castellana Grotte, ItalyNational Institute of Gastroenterology—IRCCS “Saverio de Bellis”, 70013 Castellana Grotte, ItalyNational Institute of Gastroenterology—IRCCS “Saverio de Bellis”, 70013 Castellana Grotte, ItalyAdvanced Technology Center for Aging Research, IRCCS INRCA, Via Birarelli 8, 60121 Ancona, ItalyAdvanced Technology Center for Aging Research, IRCCS INRCA, Via Birarelli 8, 60121 Ancona, ItalyDepartment of Bioscience, Biotechnology and Environment, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, ItalyNational Institute of Gastroenterology—IRCCS “Saverio de Bellis”, 70013 Castellana Grotte, ItalyNational Institute of Gastroenterology—IRCCS “Saverio de Bellis”, 70013 Castellana Grotte, ItalyNational Institute of Gastroenterology—IRCCS “Saverio de Bellis”, 70013 Castellana Grotte, ItalyNational Institute of Gastroenterology—IRCCS “Saverio de Bellis”, 70013 Castellana Grotte, ItalyDepartment of Biology, Ecology and Earth Sciences (DiBEST), University of Calabria, 87036 Rende, ItalyDepartment of Biology, Ecology and Earth Sciences (DiBEST), University of Calabria, 87036 Rende, ItalyDepartment of Biology, Ecology and Earth Sciences (DiBEST), University of Calabria, 87036 Rende, ItalyNational Institute of Gastroenterology—IRCCS “Saverio de Bellis”, 70013 Castellana Grotte, ItalyThe gut microbiota has gained increasing attention in recent years due to its significant impact on colorectal cancer (CRC) development and progression. The recent detection of bacterial DNA load in plasma holds promise as a potential non-invasive approach for early cancer detection. The aim of this study was to examine the quantity of bacterial DNA present in the plasma of 50 patients who have CRC in comparison to 40 neoplastic disease-free patients, as well as to determine if there is a correlation between the amount of plasma bacterial DNA and various clinical parameters. Plasma bacterial DNA levels were found to be elevated in the CRC group compared to the control group. As it emerged from the logistic analysis (adjusted for age and gender), these levels were strongly associated with the risk of CRC (OR = 1.02, <i>p</i> < 0.001, 95% C.I.: 1.01–1.03). Moreover, an association was identified between a reduction in tumor mass and the highest tertile of plasma bacterial DNA. Our findings indicate that individuals with CRC displayed a higher plasma bacterial DNA load compared to healthy controls. This observation lends support to the theory of heightened bacterial migration from the gastrointestinal tract to the bloodstream in CRC. Furthermore, our results establish a link between this phenomenon and the size of the tumor mass.https://www.mdpi.com/2076-2607/11/9/2360colorectal cancermicrobiotabacterial DNA |
| spellingShingle | Robertina Giacconi Rossella Donghia Graziana Arborea Maria Teresa Savino Mauro Provinciali Fabrizia Lattanzio Giusy Rita Caponio Sergio Coletta Antonia Bianco Maria Notarnicola Caterina Bonfiglio Giuseppe Passarino Patrizia D’Aquila Dina Bellizzi Pasqua Letizia Pesole Plasma Bacterial DNA Load as a Potential Biomarker for the Early Detection of Colorectal Cancer: A Case–Control Study Microorganisms colorectal cancer microbiota bacterial DNA |
| title | Plasma Bacterial DNA Load as a Potential Biomarker for the Early Detection of Colorectal Cancer: A Case–Control Study |
| title_full | Plasma Bacterial DNA Load as a Potential Biomarker for the Early Detection of Colorectal Cancer: A Case–Control Study |
| title_fullStr | Plasma Bacterial DNA Load as a Potential Biomarker for the Early Detection of Colorectal Cancer: A Case–Control Study |
| title_full_unstemmed | Plasma Bacterial DNA Load as a Potential Biomarker for the Early Detection of Colorectal Cancer: A Case–Control Study |
| title_short | Plasma Bacterial DNA Load as a Potential Biomarker for the Early Detection of Colorectal Cancer: A Case–Control Study |
| title_sort | plasma bacterial dna load as a potential biomarker for the early detection of colorectal cancer a case control study |
| topic | colorectal cancer microbiota bacterial DNA |
| url | https://www.mdpi.com/2076-2607/11/9/2360 |
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