The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila follicles
Follicle rupture, the final step in ovulation, utilizes conserved molecular mechanisms including matrix metalloproteinases (Mmps), steroid signaling, and adrenergic signaling. It is still unknown how follicles become competent for follicle rupture/ovulation. Here, we identify a zinc-finger transcrip...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-12-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/29887 |
| _version_ | 1811181110796746752 |
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| author | Lylah D Deady Wei Li Jianjun Sun |
| author_facet | Lylah D Deady Wei Li Jianjun Sun |
| author_sort | Lylah D Deady |
| collection | DOAJ |
| description | Follicle rupture, the final step in ovulation, utilizes conserved molecular mechanisms including matrix metalloproteinases (Mmps), steroid signaling, and adrenergic signaling. It is still unknown how follicles become competent for follicle rupture/ovulation. Here, we identify a zinc-finger transcription factor Hindsight (Hnt) as the first transcription factor regulating follicle’s competency for ovulation in Drosophila. Hnt is not expressed in immature stage-13 follicle cells but is upregulated in mature stage-14 follicle cells, which is essential for follicle rupture/ovulation. Hnt upregulates Mmp2 expression in posterior follicle cells (essential for the breakdown of the follicle wall) and Oamb expression in all follicle cells (the receptor for receiving adrenergic signaling and inducing Mmp2 activation). Hnt’s role in regulating Mmp2 and Oamb can be replaced by its human homolog Ras-responsive element-binding protein 1 (RREB-1). Our data suggest that Hnt/RREB-1 plays conserved role in regulating follicle maturation and competency for ovulation. |
| first_indexed | 2024-04-11T09:12:55Z |
| format | Article |
| id | doaj.art-a080cb3a51c843fdbb9203d8b533604c |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:12:55Z |
| publishDate | 2017-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a080cb3a51c843fdbb9203d8b533604c2022-12-22T04:32:26ZengeLife Sciences Publications LtdeLife2050-084X2017-12-01610.7554/eLife.29887The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila folliclesLylah D Deady0https://orcid.org/0000-0002-9316-1326Wei Li1https://orcid.org/0000-0001-8699-5325Jianjun Sun2https://orcid.org/0000-0002-6015-738XDepartment of Physiology and Neurobiology, University of Connecticut, Connecticut, United StatesDepartment of Physiology and Neurobiology, University of Connecticut, Connecticut, United StatesDepartment of Physiology and Neurobiology, University of Connecticut, Connecticut, United States; Institute for Systems Genomics, University of Connecticut, Connecticut, United StatesFollicle rupture, the final step in ovulation, utilizes conserved molecular mechanisms including matrix metalloproteinases (Mmps), steroid signaling, and adrenergic signaling. It is still unknown how follicles become competent for follicle rupture/ovulation. Here, we identify a zinc-finger transcription factor Hindsight (Hnt) as the first transcription factor regulating follicle’s competency for ovulation in Drosophila. Hnt is not expressed in immature stage-13 follicle cells but is upregulated in mature stage-14 follicle cells, which is essential for follicle rupture/ovulation. Hnt upregulates Mmp2 expression in posterior follicle cells (essential for the breakdown of the follicle wall) and Oamb expression in all follicle cells (the receptor for receiving adrenergic signaling and inducing Mmp2 activation). Hnt’s role in regulating Mmp2 and Oamb can be replaced by its human homolog Ras-responsive element-binding protein 1 (RREB-1). Our data suggest that Hnt/RREB-1 plays conserved role in regulating follicle maturation and competency for ovulation.https://elifesciences.org/articles/29887follicle maturationovulationHindsightRREB-1Mmp2Oamb |
| spellingShingle | Lylah D Deady Wei Li Jianjun Sun The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila follicles eLife follicle maturation ovulation Hindsight RREB-1 Mmp2 Oamb |
| title | The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila follicles |
| title_full | The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila follicles |
| title_fullStr | The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila follicles |
| title_full_unstemmed | The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila follicles |
| title_short | The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila follicles |
| title_sort | zinc finger transcription factor hindsight regulates ovulation competency of drosophila follicles |
| topic | follicle maturation ovulation Hindsight RREB-1 Mmp2 Oamb |
| url | https://elifesciences.org/articles/29887 |
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