The Caucasian Clover Gene <i>TaMYC2</i> Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant Enzymes
Abiotic stress affects metabolic processes in plants and restricts plant growth and development. In this experiment, Caucasian clover (<i>Trifolium ambiguum</i> M. Bieb.) was used as a material, and the CDS of <i>TaMYC2</i>, which is involved in regulating the response to abi...
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MDPI AG
2022-02-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/2/329 |
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| author | Yihang Zhao Yupeng Yang Jingwen Jiang Xiaomeng Zhang Zewang Ma Lingdong Meng Guowen Cui Xiujie Yin |
| author_facet | Yihang Zhao Yupeng Yang Jingwen Jiang Xiaomeng Zhang Zewang Ma Lingdong Meng Guowen Cui Xiujie Yin |
| author_sort | Yihang Zhao |
| collection | DOAJ |
| description | Abiotic stress affects metabolic processes in plants and restricts plant growth and development. In this experiment, Caucasian clover (<i>Trifolium ambiguum</i> M. Bieb.) was used as a material, and the CDS of <i>TaMYC2</i>, which is involved in regulating the response to abiotic stress, was cloned. The CDS of <i>TaMYC2</i> was 726 bp in length and encoded 241 amino acids. The protein encoded by <i>TaMYC2</i> was determined to be unstable, be highly hydrophilic, and contain 23 phosphorylation sites. Subcellular localization results showed that <i>TaMYC2</i> was localized in the nucleus. <i>TaMYC2</i> responded to salt, alkali, cold, and drought stress and could be induced by IAA, GA<sub>3</sub>, and MeJA. By analyzing the gene expression and antioxidant enzyme activity in plants before and after stress, we found that drought and cold stress could induce the expression of <i>TaMYC2</i> and increase the antioxidant enzyme activity. <i>TaMYC2</i> could also induce the expression of ROS scavenging-related and stress-responsive genes and increase the activity of antioxidant enzymes, thus improving the ability of plants to resist stress. The results of this experiment provide references for subsequent in-depth exploration of both the function of <i>TaMYC2</i> in and the molecular mechanism underlying the resistance of Caucasian clover. |
| first_indexed | 2024-03-09T21:52:03Z |
| format | Article |
| id | doaj.art-a084864b239441b3962e723bc1281f59 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T21:52:03Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-a084864b239441b3962e723bc1281f592023-11-23T20:05:10ZengMDPI AGGenes2073-44252022-02-0113232910.3390/genes13020329The Caucasian Clover Gene <i>TaMYC2</i> Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant EnzymesYihang Zhao0Yupeng Yang1Jingwen Jiang2Xiaomeng Zhang3Zewang Ma4Lingdong Meng5Guowen Cui6Xiujie Yin7College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaAbiotic stress affects metabolic processes in plants and restricts plant growth and development. In this experiment, Caucasian clover (<i>Trifolium ambiguum</i> M. Bieb.) was used as a material, and the CDS of <i>TaMYC2</i>, which is involved in regulating the response to abiotic stress, was cloned. The CDS of <i>TaMYC2</i> was 726 bp in length and encoded 241 amino acids. The protein encoded by <i>TaMYC2</i> was determined to be unstable, be highly hydrophilic, and contain 23 phosphorylation sites. Subcellular localization results showed that <i>TaMYC2</i> was localized in the nucleus. <i>TaMYC2</i> responded to salt, alkali, cold, and drought stress and could be induced by IAA, GA<sub>3</sub>, and MeJA. By analyzing the gene expression and antioxidant enzyme activity in plants before and after stress, we found that drought and cold stress could induce the expression of <i>TaMYC2</i> and increase the antioxidant enzyme activity. <i>TaMYC2</i> could also induce the expression of ROS scavenging-related and stress-responsive genes and increase the activity of antioxidant enzymes, thus improving the ability of plants to resist stress. The results of this experiment provide references for subsequent in-depth exploration of both the function of <i>TaMYC2</i> in and the molecular mechanism underlying the resistance of Caucasian clover.https://www.mdpi.com/2073-4425/13/2/329Caucasian clover<i>TaMYC2</i>abiotic stresscloneantioxidant enzyme |
| spellingShingle | Yihang Zhao Yupeng Yang Jingwen Jiang Xiaomeng Zhang Zewang Ma Lingdong Meng Guowen Cui Xiujie Yin The Caucasian Clover Gene <i>TaMYC2</i> Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant Enzymes Genes Caucasian clover <i>TaMYC2</i> abiotic stress clone antioxidant enzyme |
| title | The Caucasian Clover Gene <i>TaMYC2</i> Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant Enzymes |
| title_full | The Caucasian Clover Gene <i>TaMYC2</i> Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant Enzymes |
| title_fullStr | The Caucasian Clover Gene <i>TaMYC2</i> Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant Enzymes |
| title_full_unstemmed | The Caucasian Clover Gene <i>TaMYC2</i> Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant Enzymes |
| title_short | The Caucasian Clover Gene <i>TaMYC2</i> Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant Enzymes |
| title_sort | caucasian clover gene i tamyc2 i responds to abiotic stress and improves tolerance by increasing the activity of antioxidant enzymes |
| topic | Caucasian clover <i>TaMYC2</i> abiotic stress clone antioxidant enzyme |
| url | https://www.mdpi.com/2073-4425/13/2/329 |
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