Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI
To assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the intern...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/12/6/2395 |
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| author | Reza Jalli Davood Mehrabani Shahrokh Zare Mahdi Saeedi Moghadam Iman Jamhiri Navid Manafi Golshid Mehrabani Janan Ghabanchi Iman Razeghian Jahromi Aghdass Rasouli-Nia Feridoun Karimi-Busheri |
| author_facet | Reza Jalli Davood Mehrabani Shahrokh Zare Mahdi Saeedi Moghadam Iman Jamhiri Navid Manafi Golshid Mehrabani Janan Ghabanchi Iman Razeghian Jahromi Aghdass Rasouli-Nia Feridoun Karimi-Busheri |
| author_sort | Reza Jalli |
| collection | DOAJ |
| description | To assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the internalization of these nanoparticles. Cell proliferation, viability, differentiation, and apoptosis were investigated. Prussian blue staining and MRI were used to trace transfected labeled cells. DPSCs were a morphologically spindle shape, adherent to culture plates, and positive for adipogenic and osteogenic inductions. They expressed CD73 and CD90 markers and lacked CD34 and CD45. Iron oxide labeling and transfection with lipofectamine in DPSCs had no toxic impact on viability, proliferation, and differentiation, and did not induce any apoptosis. In vitro and in vivo internalization of iron oxide nanoparticles within DPSCs were confirmed by Prussian blue staining and MRI tracking. Prussian blue staining and MRI tracking in the absence of any toxic effects on cell viability, proliferation, differentiation, and apoptosis were safe and accurate to track DPSCs labeled with iron oxide and transfected with lipofectamine. MRI can be a useful imaging modality when treatment outcome is targeted. |
| first_indexed | 2024-03-11T06:22:17Z |
| format | Article |
| id | doaj.art-a087193032dd453ea9ae05753808c995 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-11T06:22:17Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-a087193032dd453ea9ae05753808c9952023-11-17T11:52:20ZengMDPI AGJournal of Clinical Medicine2077-03832023-03-01126239510.3390/jcm12062395Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRIReza Jalli0Davood Mehrabani1Shahrokh Zare2Mahdi Saeedi Moghadam3Iman Jamhiri4Navid Manafi5Golshid Mehrabani6Janan Ghabanchi7Iman Razeghian Jahromi8Aghdass Rasouli-Nia9Feridoun Karimi-Busheri10Medical Imaging Research Center, Shiraz University of Medical Sciences, Shiraz 71439-14693, IranStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz 71439-14693, IranStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz 71439-14693, IranMedical Imaging Research Center, Shiraz University of Medical Sciences, Shiraz 71439-14693, IranStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz 71439-14693, IranSchool of Medicine, Zanjan University of Medical Sciences, Zanjan 71439-14693, IranSchool of Dentistry, Shiraz University of Medical Sciences, Shiraz 71439-14693, IranSchool of Dentistry, Shiraz University of Medical Sciences, Shiraz 71439-14693, IranStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz 71439-14693, IranDepartment of Oncology, Cross Cancer Institute, Faculty of Medicine, University of Alberta, Edmonton, AB T6G 1H9, CanadaDepartment of Oncology, Cross Cancer Institute, Faculty of Medicine, University of Alberta, Edmonton, AB T6G 1H9, CanadaTo assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the internalization of these nanoparticles. Cell proliferation, viability, differentiation, and apoptosis were investigated. Prussian blue staining and MRI were used to trace transfected labeled cells. DPSCs were a morphologically spindle shape, adherent to culture plates, and positive for adipogenic and osteogenic inductions. They expressed CD73 and CD90 markers and lacked CD34 and CD45. Iron oxide labeling and transfection with lipofectamine in DPSCs had no toxic impact on viability, proliferation, and differentiation, and did not induce any apoptosis. In vitro and in vivo internalization of iron oxide nanoparticles within DPSCs were confirmed by Prussian blue staining and MRI tracking. Prussian blue staining and MRI tracking in the absence of any toxic effects on cell viability, proliferation, differentiation, and apoptosis were safe and accurate to track DPSCs labeled with iron oxide and transfected with lipofectamine. MRI can be a useful imaging modality when treatment outcome is targeted.https://www.mdpi.com/2077-0383/12/6/2395dental pulp stem cellsiron oxide nanoparticleslipofectaminetrackingMRI |
| spellingShingle | Reza Jalli Davood Mehrabani Shahrokh Zare Mahdi Saeedi Moghadam Iman Jamhiri Navid Manafi Golshid Mehrabani Janan Ghabanchi Iman Razeghian Jahromi Aghdass Rasouli-Nia Feridoun Karimi-Busheri Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI Journal of Clinical Medicine dental pulp stem cells iron oxide nanoparticles lipofectamine tracking MRI |
| title | Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI |
| title_full | Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI |
| title_fullStr | Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI |
| title_full_unstemmed | Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI |
| title_short | Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI |
| title_sort | cell proliferation viability differentiation and apoptosis of iron oxide labeled stem cells transfected with lipofectamine assessed by mri |
| topic | dental pulp stem cells iron oxide nanoparticles lipofectamine tracking MRI |
| url | https://www.mdpi.com/2077-0383/12/6/2395 |
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