Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum
Insect chitinases play a crucial part to digest chitin in the exoskeleton during the molting process. However, research on insect chitinase related to the environmental stress response is very limited. This study was the first conducted to expression analysis of chitinase- related genes in A. pisum...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.1024136/full |
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| author | Chunchun Li Inzamam Ul Haq Aroosa Khurshid Yan Tao Peter Quandahor Peter Quandahor Jing-Jiang Zhou Jing-Jiang Zhou Chang-Zhong Liu |
| author_facet | Chunchun Li Inzamam Ul Haq Aroosa Khurshid Yan Tao Peter Quandahor Peter Quandahor Jing-Jiang Zhou Jing-Jiang Zhou Chang-Zhong Liu |
| author_sort | Chunchun Li |
| collection | DOAJ |
| description | Insect chitinases play a crucial part to digest chitin in the exoskeleton during the molting process. However, research on insect chitinase related to the environmental stress response is very limited. This study was the first conducted to expression analysis of chitinase- related genes in A. pisum under abiotic stresses. Here, we identified five chitinase-like proteins (ApIDGF, ApCht3, ApCht7, ApCht10 and ApENGase), and clustered them into five groups (group II, III, V, Ⅹ, and ENGase). Developmental expression analysis revealed that the five A. pisum chitinase-related genes were expressed at whole developmental stages with different relative expression patterns. When aphids were exposed to various abiotic stresses including temperature, insecticide and the stress 20-hydroxyecdysone (20E), all five chitinase genes were differentially expressed in A. pisum. The results showed that insecticide such as imidacloprid down-regulated the expression of these five Cht-related genes. Analysis of temperature stress of A. pisum chitinase suggested that ApCht7 expression was high at 10°C, which demonstrates its important role in pea aphids under low temperature. Conversely, ApCht10 was more active under high temperature stress, as it was significantly up-regulated at 30°C. Besides, 20E enhanced ApCht3 and ApCht10 expression in A. pisum, but reduced ApCht7 expression. These findings provide basic information and insights for the study of the role of these genes under abiotic stress, which advances our knowledge in the management of pea aphids under multiple stresses. |
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| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-11T15:01:55Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-a08c1c74e9b54282a36899a1f91603992022-12-22T04:16:55ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-11-011310.3389/fphys.2022.10241361024136Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisumChunchun Li0Inzamam Ul Haq1Aroosa Khurshid2Yan Tao3Peter Quandahor4Peter Quandahor5Jing-Jiang Zhou6Jing-Jiang Zhou7Chang-Zhong Liu8Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou, ChinaBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou, ChinaBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou, ChinaBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou, ChinaBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou, ChinaCSIR-Savanna Agricultural Research Institute, Tamale, GhanaBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Green Pesticide and Agricultural Bioengineering, Guizhou University, Guiyang, ChinaBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou, ChinaInsect chitinases play a crucial part to digest chitin in the exoskeleton during the molting process. However, research on insect chitinase related to the environmental stress response is very limited. This study was the first conducted to expression analysis of chitinase- related genes in A. pisum under abiotic stresses. Here, we identified five chitinase-like proteins (ApIDGF, ApCht3, ApCht7, ApCht10 and ApENGase), and clustered them into five groups (group II, III, V, Ⅹ, and ENGase). Developmental expression analysis revealed that the five A. pisum chitinase-related genes were expressed at whole developmental stages with different relative expression patterns. When aphids were exposed to various abiotic stresses including temperature, insecticide and the stress 20-hydroxyecdysone (20E), all five chitinase genes were differentially expressed in A. pisum. The results showed that insecticide such as imidacloprid down-regulated the expression of these five Cht-related genes. Analysis of temperature stress of A. pisum chitinase suggested that ApCht7 expression was high at 10°C, which demonstrates its important role in pea aphids under low temperature. Conversely, ApCht10 was more active under high temperature stress, as it was significantly up-regulated at 30°C. Besides, 20E enhanced ApCht3 and ApCht10 expression in A. pisum, but reduced ApCht7 expression. These findings provide basic information and insights for the study of the role of these genes under abiotic stress, which advances our knowledge in the management of pea aphids under multiple stresses.https://www.frontiersin.org/articles/10.3389/fphys.2022.1024136/fullAcyrthosiphon pisumchitinaseexpression profilesphylogenetic analysis20hydroxyecdysone |
| spellingShingle | Chunchun Li Inzamam Ul Haq Aroosa Khurshid Yan Tao Peter Quandahor Peter Quandahor Jing-Jiang Zhou Jing-Jiang Zhou Chang-Zhong Liu Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum Frontiers in Physiology Acyrthosiphon pisum chitinase expression profiles phylogenetic analysis 20hydroxyecdysone |
| title | Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum |
| title_full | Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum |
| title_fullStr | Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum |
| title_full_unstemmed | Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum |
| title_short | Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum |
| title_sort | effects of abiotic stresses on the expression of chitinase like genes in acyrthosiphon pisum |
| topic | Acyrthosiphon pisum chitinase expression profiles phylogenetic analysis 20hydroxyecdysone |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.1024136/full |
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