Relative Contribution of Blood Pressure and Renal Sympathetic Nerve Activity to Proximal Tubular Sodium Reabsorption via NHE3 Activity
We examined the effects of an acute increase in blood pressure (BP) and renal sympathetic nerve activity (rSNA) induced by bicuculline (Bic) injection in the paraventricular nucleus of hypothalamus (PVN) or the effects of a selective increase in rSNA induced by renal nerve stimulation (RNS) on the r...
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/1/349 |
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| author | Roberto B. Pontes Erika E. Nishi Renato O. Crajoinas Maycon I. O. Milanez Adriana C. C. Girardi Ruy R Campos Cassia T Bergamaschi |
| author_facet | Roberto B. Pontes Erika E. Nishi Renato O. Crajoinas Maycon I. O. Milanez Adriana C. C. Girardi Ruy R Campos Cassia T Bergamaschi |
| author_sort | Roberto B. Pontes |
| collection | DOAJ |
| description | We examined the effects of an acute increase in blood pressure (BP) and renal sympathetic nerve activity (rSNA) induced by bicuculline (Bic) injection in the paraventricular nucleus of hypothalamus (PVN) or the effects of a selective increase in rSNA induced by renal nerve stimulation (RNS) on the renal excretion of sodium and water and its effect on sodium-hydrogen exchanger 3 (NHE3) activity. Uninephrectomized anesthetized male Wistar rats were divided into three groups: (1) Sham; (2) Bic PVN: (3) RNS + Bic injection into the PVN. BP and rSNA were recorded, and urine was collected prior and after the interventions in all groups. RNS decreased sodium (58%) and water excretion (53%) independently of BP changes (<i>p</i> < 0.05). However, after Bic injection in the PVN during RNS stimulation, the BP and rSNA increased by 30% and 60% (<i>p</i> < 0.05), respectively, diuresis (5-fold) and natriuresis (2.3-fold) were increased (<i>p</i> < 0.05), and NHE3 activity was significantly reduced, independently of glomerular filtration rate changes. Thus, an acute increase in the BP overcomes RNS, leading to diuresis, natriuresis, and NHE3 activity inhibition. |
| first_indexed | 2024-03-11T10:00:03Z |
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| id | doaj.art-a08e542e617d48b7b6fad64a867ddcca |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T10:00:03Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a08e542e617d48b7b6fad64a867ddcca2023-11-16T15:32:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124134910.3390/ijms24010349Relative Contribution of Blood Pressure and Renal Sympathetic Nerve Activity to Proximal Tubular Sodium Reabsorption via NHE3 ActivityRoberto B. Pontes0Erika E. Nishi1Renato O. Crajoinas2Maycon I. O. Milanez3Adriana C. C. Girardi4Ruy R Campos5Cassia T Bergamaschi6Cardiovascular Division, Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04021-001, BrazilCardiovascular Division, Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04021-001, BrazilHeart Institute (InCor), University of São Paulo Medical School, São Paulo 01246-903, BrazilCardiovascular Division, Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04021-001, BrazilHeart Institute (InCor), University of São Paulo Medical School, São Paulo 01246-903, BrazilCardiovascular Division, Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04021-001, BrazilCardiovascular Division, Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04021-001, BrazilWe examined the effects of an acute increase in blood pressure (BP) and renal sympathetic nerve activity (rSNA) induced by bicuculline (Bic) injection in the paraventricular nucleus of hypothalamus (PVN) or the effects of a selective increase in rSNA induced by renal nerve stimulation (RNS) on the renal excretion of sodium and water and its effect on sodium-hydrogen exchanger 3 (NHE3) activity. Uninephrectomized anesthetized male Wistar rats were divided into three groups: (1) Sham; (2) Bic PVN: (3) RNS + Bic injection into the PVN. BP and rSNA were recorded, and urine was collected prior and after the interventions in all groups. RNS decreased sodium (58%) and water excretion (53%) independently of BP changes (<i>p</i> < 0.05). However, after Bic injection in the PVN during RNS stimulation, the BP and rSNA increased by 30% and 60% (<i>p</i> < 0.05), respectively, diuresis (5-fold) and natriuresis (2.3-fold) were increased (<i>p</i> < 0.05), and NHE3 activity was significantly reduced, independently of glomerular filtration rate changes. Thus, an acute increase in the BP overcomes RNS, leading to diuresis, natriuresis, and NHE3 activity inhibition.https://www.mdpi.com/1422-0067/24/1/349NHE3glomerular filtration ratebicucullineparaventricular nucleus of the hypothalamussympathetic nerve activity |
| spellingShingle | Roberto B. Pontes Erika E. Nishi Renato O. Crajoinas Maycon I. O. Milanez Adriana C. C. Girardi Ruy R Campos Cassia T Bergamaschi Relative Contribution of Blood Pressure and Renal Sympathetic Nerve Activity to Proximal Tubular Sodium Reabsorption via NHE3 Activity International Journal of Molecular Sciences NHE3 glomerular filtration rate bicuculline paraventricular nucleus of the hypothalamus sympathetic nerve activity |
| title | Relative Contribution of Blood Pressure and Renal Sympathetic Nerve Activity to Proximal Tubular Sodium Reabsorption via NHE3 Activity |
| title_full | Relative Contribution of Blood Pressure and Renal Sympathetic Nerve Activity to Proximal Tubular Sodium Reabsorption via NHE3 Activity |
| title_fullStr | Relative Contribution of Blood Pressure and Renal Sympathetic Nerve Activity to Proximal Tubular Sodium Reabsorption via NHE3 Activity |
| title_full_unstemmed | Relative Contribution of Blood Pressure and Renal Sympathetic Nerve Activity to Proximal Tubular Sodium Reabsorption via NHE3 Activity |
| title_short | Relative Contribution of Blood Pressure and Renal Sympathetic Nerve Activity to Proximal Tubular Sodium Reabsorption via NHE3 Activity |
| title_sort | relative contribution of blood pressure and renal sympathetic nerve activity to proximal tubular sodium reabsorption via nhe3 activity |
| topic | NHE3 glomerular filtration rate bicuculline paraventricular nucleus of the hypothalamus sympathetic nerve activity |
| url | https://www.mdpi.com/1422-0067/24/1/349 |
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