Productive infection of HUVEC by HHV-8 is associated with changes compatible with angiogenic transformation
Kaposi’s Sarcoma (KS) is an angioproliferative disease associated with human herpesvirus 8 (HHV-8) infection.We have characterized the morphologic and phenotypic modifications of HUVEC in a model of productive HHV-8 infection. HHV-8 replication was associated with ultra-structural changes, flattened...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2009-06-01
|
| Series: | European Journal of Histochemistry |
| Online Access: | https://www.ejh.it/index.php/ejh/article/view/954 |
| _version_ | 1819037694196449280 |
|---|---|
| author | C Foglieni S Scabini D Belloni F Broccolo P Lusso MS Malnati E Ferrero |
| author_facet | C Foglieni S Scabini D Belloni F Broccolo P Lusso MS Malnati E Ferrero |
| author_sort | C Foglieni |
| collection | DOAJ |
| description | Kaposi’s Sarcoma (KS) is an angioproliferative disease associated with human herpesvirus 8 (HHV-8) infection.We have characterized the morphologic and phenotypic modifications of HUVEC in a model of productive HHV-8 infection. HHV-8 replication was associated with ultra-structural changes, flattened soma and a loss of marginal folds and intercellular contacts, and morphologic features, spindle cell conversion and cordon-like structures formation. Phenotypic changes observed on cordon-like structures included partial loss and redistribution of CD31/PECAM-1 and VE-cadherin, uPAR upregulation and de novo expression of CD13/APN. Such changes demonstrate the induction, in HUVEC, of an angiogenic profile. Most of these findings are directly linked to HHV-8-encoded proteins expression, suggesting that HHV-8 itself may participate to the initial steps of the angiogenic transformation in KS. |
| first_indexed | 2024-12-21T08:25:29Z |
| format | Article |
| id | doaj.art-a0902fa7250f4b22b741fe7d50b7c6c4 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-21T08:25:29Z |
| publishDate | 2009-06-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-a0902fa7250f4b22b741fe7d50b7c6c42022-12-21T19:10:20ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062009-06-0149310.4081/954673Productive infection of HUVEC by HHV-8 is associated with changes compatible with angiogenic transformationC FoglieniS ScabiniD BelloniF BroccoloP LussoMS MalnatiE FerreroKaposi’s Sarcoma (KS) is an angioproliferative disease associated with human herpesvirus 8 (HHV-8) infection.We have characterized the morphologic and phenotypic modifications of HUVEC in a model of productive HHV-8 infection. HHV-8 replication was associated with ultra-structural changes, flattened soma and a loss of marginal folds and intercellular contacts, and morphologic features, spindle cell conversion and cordon-like structures formation. Phenotypic changes observed on cordon-like structures included partial loss and redistribution of CD31/PECAM-1 and VE-cadherin, uPAR upregulation and de novo expression of CD13/APN. Such changes demonstrate the induction, in HUVEC, of an angiogenic profile. Most of these findings are directly linked to HHV-8-encoded proteins expression, suggesting that HHV-8 itself may participate to the initial steps of the angiogenic transformation in KS.https://www.ejh.it/index.php/ejh/article/view/954 |
| spellingShingle | C Foglieni S Scabini D Belloni F Broccolo P Lusso MS Malnati E Ferrero Productive infection of HUVEC by HHV-8 is associated with changes compatible with angiogenic transformation European Journal of Histochemistry |
| title | Productive infection of HUVEC by HHV-8 is associated with changes compatible with angiogenic transformation |
| title_full | Productive infection of HUVEC by HHV-8 is associated with changes compatible with angiogenic transformation |
| title_fullStr | Productive infection of HUVEC by HHV-8 is associated with changes compatible with angiogenic transformation |
| title_full_unstemmed | Productive infection of HUVEC by HHV-8 is associated with changes compatible with angiogenic transformation |
| title_short | Productive infection of HUVEC by HHV-8 is associated with changes compatible with angiogenic transformation |
| title_sort | productive infection of huvec by hhv 8 is associated with changes compatible with angiogenic transformation |
| url | https://www.ejh.it/index.php/ejh/article/view/954 |
| work_keys_str_mv | AT cfoglieni productiveinfectionofhuvecbyhhv8isassociatedwithchangescompatiblewithangiogenictransformation AT sscabini productiveinfectionofhuvecbyhhv8isassociatedwithchangescompatiblewithangiogenictransformation AT dbelloni productiveinfectionofhuvecbyhhv8isassociatedwithchangescompatiblewithangiogenictransformation AT fbroccolo productiveinfectionofhuvecbyhhv8isassociatedwithchangescompatiblewithangiogenictransformation AT plusso productiveinfectionofhuvecbyhhv8isassociatedwithchangescompatiblewithangiogenictransformation AT msmalnati productiveinfectionofhuvecbyhhv8isassociatedwithchangescompatiblewithangiogenictransformation AT eferrero productiveinfectionofhuvecbyhhv8isassociatedwithchangescompatiblewithangiogenictransformation |