A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses
The plant hormone abscisic acid (ABA) modulates a number of plant developmental processes and responses to stress. In planta, ABA has been shown to induce reactive oxygen species (ROS) production through the action of plasma membrane-associated nicotinamide adenine dinucleotide phosphate (NADPH)-oxi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-08-01
|
| Series: | Proteomes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-7382/5/3/22 |
| _version_ | 1798034870257057792 |
|---|---|
| author | May Alqurashi Ludivine Thomas Chris Gehring Claudius Marondedze |
| author_facet | May Alqurashi Ludivine Thomas Chris Gehring Claudius Marondedze |
| author_sort | May Alqurashi |
| collection | DOAJ |
| description | The plant hormone abscisic acid (ABA) modulates a number of plant developmental processes and responses to stress. In planta, ABA has been shown to induce reactive oxygen species (ROS) production through the action of plasma membrane-associated nicotinamide adenine dinucleotide phosphate (NADPH)-oxidases. Although quantitative proteomics studies have been performed to identify ABA- or hydrogen peroxide (H2O2)-dependent proteins, little is known about the ABA- and H2O2-dependent microsomal proteome changes. Here, we examined the effect of 50 µM of either H2O2 or ABA on the Arabidopsis microsomal proteome using tandem mass spectrometry and identified 86 specifically H2O2-dependent, and 52 specifically ABA-dependent proteins that are differentially expressed. We observed differential accumulation of proteins involved in the tricarboxylic acid (TCA) cycle notably in response to H2O2. Of these, aconitase 3 responded to both H2O2 and ABA. Additionally, over 30 proteins linked to RNA biology responded significantly to both treatments. Gene ontology categories such as ‘response to stress’ and ‘transport’ were enriched, suggesting that H2O2 or ABA directly and/or indirectly cause complex and partly overlapping cellular responses. Data are available via ProteomeXchange with identifier PXD006513. |
| first_indexed | 2024-04-11T20:50:23Z |
| format | Article |
| id | doaj.art-a091b10543f54bd48034a3918ddfdf87 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-04-11T20:50:23Z |
| publishDate | 2017-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-a091b10543f54bd48034a3918ddfdf872022-12-22T04:03:52ZengMDPI AGProteomes2227-73822017-08-01532210.3390/proteomes5030022proteomes5030022A Microsomal Proteomics View of H2O2- and ABA-Dependent ResponsesMay Alqurashi0Ludivine Thomas1Chris Gehring2Claudius Marondedze3Cambridge Centre for Proteomics, Cambridge Systems Biology Centre, Department of Biochemistry, University of Cambridge Tennis Court Road, Cambridge CB2 1QR, UKHM. Clause, rue Louis Saillant, Z.I. La Motte, BP83, 26802 Portes-lès-Valence, FranceBiological and Environmental Sciences & Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi ArabiaCambridge Centre for Proteomics, Cambridge Systems Biology Centre, Department of Biochemistry, University of Cambridge Tennis Court Road, Cambridge CB2 1QR, UKThe plant hormone abscisic acid (ABA) modulates a number of plant developmental processes and responses to stress. In planta, ABA has been shown to induce reactive oxygen species (ROS) production through the action of plasma membrane-associated nicotinamide adenine dinucleotide phosphate (NADPH)-oxidases. Although quantitative proteomics studies have been performed to identify ABA- or hydrogen peroxide (H2O2)-dependent proteins, little is known about the ABA- and H2O2-dependent microsomal proteome changes. Here, we examined the effect of 50 µM of either H2O2 or ABA on the Arabidopsis microsomal proteome using tandem mass spectrometry and identified 86 specifically H2O2-dependent, and 52 specifically ABA-dependent proteins that are differentially expressed. We observed differential accumulation of proteins involved in the tricarboxylic acid (TCA) cycle notably in response to H2O2. Of these, aconitase 3 responded to both H2O2 and ABA. Additionally, over 30 proteins linked to RNA biology responded significantly to both treatments. Gene ontology categories such as ‘response to stress’ and ‘transport’ were enriched, suggesting that H2O2 or ABA directly and/or indirectly cause complex and partly overlapping cellular responses. Data are available via ProteomeXchange with identifier PXD006513.https://www.mdpi.com/2227-7382/5/3/22hydrogen peroxide (H2O2)abscisic acid (ABA)microsomal proteomicsquantitative proteomicsmass spectrometry |
| spellingShingle | May Alqurashi Ludivine Thomas Chris Gehring Claudius Marondedze A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses Proteomes hydrogen peroxide (H2O2) abscisic acid (ABA) microsomal proteomics quantitative proteomics mass spectrometry |
| title | A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses |
| title_full | A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses |
| title_fullStr | A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses |
| title_full_unstemmed | A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses |
| title_short | A Microsomal Proteomics View of H2O2- and ABA-Dependent Responses |
| title_sort | microsomal proteomics view of h2o2 and aba dependent responses |
| topic | hydrogen peroxide (H2O2) abscisic acid (ABA) microsomal proteomics quantitative proteomics mass spectrometry |
| url | https://www.mdpi.com/2227-7382/5/3/22 |
| work_keys_str_mv | AT mayalqurashi amicrosomalproteomicsviewofh2o2andabadependentresponses AT ludivinethomas amicrosomalproteomicsviewofh2o2andabadependentresponses AT chrisgehring amicrosomalproteomicsviewofh2o2andabadependentresponses AT claudiusmarondedze amicrosomalproteomicsviewofh2o2andabadependentresponses AT mayalqurashi microsomalproteomicsviewofh2o2andabadependentresponses AT ludivinethomas microsomalproteomicsviewofh2o2andabadependentresponses AT chrisgehring microsomalproteomicsviewofh2o2andabadependentresponses AT claudiusmarondedze microsomalproteomicsviewofh2o2andabadependentresponses |