Phage Biocontrol of Bacterial Leaf Blight Disease on Welsh Onion Caused by <i>Xanthomonas axonopodis</i> pv. <i>allii</i>
Bacterial leaf blight, which is caused by <i>Xanthomonas axonopodis</i> pv. <i>allii</i>, annually causes significant yield losses to Welsh onion in many producing countries, including Vietnam. In this study, we isolated and characterized lytic phages Φ16, Φ17A and Φ31, speci...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/5/517 |
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| author | Nguyen Thi Thu Nga Tran Ngoc Tran Dominique Holtappels Nguyen Le Kim Ngan Nguyen Phuoc Hao Marta Vallino Doan Thi Kieu Tien Nguyen Huan Khanh-Pham Rob Lavigne Kaeko Kamei Jeroen Wagemans Jeffrey B. Jones |
| author_facet | Nguyen Thi Thu Nga Tran Ngoc Tran Dominique Holtappels Nguyen Le Kim Ngan Nguyen Phuoc Hao Marta Vallino Doan Thi Kieu Tien Nguyen Huan Khanh-Pham Rob Lavigne Kaeko Kamei Jeroen Wagemans Jeffrey B. Jones |
| author_sort | Nguyen Thi Thu Nga |
| collection | DOAJ |
| description | Bacterial leaf blight, which is caused by <i>Xanthomonas axonopodis</i> pv. <i>allii</i>, annually causes significant yield losses to Welsh onion in many producing countries, including Vietnam. In this study, we isolated and characterized lytic phages Φ16, Φ17A and Φ31, specific to <i>X. axonopodis</i> pv. <i>allii</i> and belonging to a new phage species and genus within the <i>Autographiviridae</i>, from four provinces in the Mekong Delta of Vietnam. Moreover, we evaluated their efficacy for the biocontrol of leaf blight in greenhouse and field conditions. When applying the three highly related phages individually or as a three-phage cocktail at 10<sup>8</sup> PFU/mL in greenhouse conditions, our results show that treatment with Φ31 alone provides higher disease prevention than the two other phages or the phage cocktail. Furthermore, we compared phage concentrations from 10<sup>5</sup> to 10<sup>8</sup> and showed optimal disease control at 10<sup>7</sup> and 10<sup>8</sup> PFU/mL. Finally, under field conditions, both phage Φ31 alone and the phage cocktail treatments suppressed disease symptoms, which was comparable to the chemical bactericide oxolinic acid (Starner). Phage treatment also significantly improved yield, showing the potential of phage as a biocontrol strategy for managing leaf blight in Welsh onion. |
| first_indexed | 2024-03-10T11:45:28Z |
| format | Article |
| id | doaj.art-a09395a0bdcd4c518b0f2fbdcfd5ce94 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T11:45:28Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a09395a0bdcd4c518b0f2fbdcfd5ce942023-11-21T18:09:39ZengMDPI AGAntibiotics2079-63822021-05-0110551710.3390/antibiotics10050517Phage Biocontrol of Bacterial Leaf Blight Disease on Welsh Onion Caused by <i>Xanthomonas axonopodis</i> pv. <i>allii</i>Nguyen Thi Thu Nga0Tran Ngoc Tran1Dominique Holtappels2Nguyen Le Kim Ngan3Nguyen Phuoc Hao4Marta Vallino5Doan Thi Kieu Tien6Nguyen Huan Khanh-Pham7Rob Lavigne8Kaeko Kamei9Jeroen Wagemans10Jeffrey B. Jones11Department of Plant Protection, College of Agriculture, Can Tho University, Can Tho 90000, VietnamDepartment of Plant Protection, College of Agriculture, Can Tho University, Can Tho 90000, VietnamDepartment of Biosystems, KU Leuven, 3001 Leuven, BelgiumDepartment of Plant Protection, College of Agriculture, Can Tho University, Can Tho 90000, VietnamDepartment of Plant Protection, College of Agriculture, Can Tho University, Can Tho 90000, VietnamInstitute for Sustainable Plant Protection, National Research Council of Italy, 10135 Turin, ItalyDepartment of Plant Protection, College of Agriculture, Can Tho University, Can Tho 90000, VietnamDepartment of Biology, College of Natural Science, Can Tho University, Can Tho 90000, VietnamDepartment of Biosystems, KU Leuven, 3001 Leuven, BelgiumDepartment of Biomolecular Engineering, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, JapanDepartment of Biosystems, KU Leuven, 3001 Leuven, BelgiumDepartment of Plant Pathology, University of Florida, Gainesville, FL 32611, USABacterial leaf blight, which is caused by <i>Xanthomonas axonopodis</i> pv. <i>allii</i>, annually causes significant yield losses to Welsh onion in many producing countries, including Vietnam. In this study, we isolated and characterized lytic phages Φ16, Φ17A and Φ31, specific to <i>X. axonopodis</i> pv. <i>allii</i> and belonging to a new phage species and genus within the <i>Autographiviridae</i>, from four provinces in the Mekong Delta of Vietnam. Moreover, we evaluated their efficacy for the biocontrol of leaf blight in greenhouse and field conditions. When applying the three highly related phages individually or as a three-phage cocktail at 10<sup>8</sup> PFU/mL in greenhouse conditions, our results show that treatment with Φ31 alone provides higher disease prevention than the two other phages or the phage cocktail. Furthermore, we compared phage concentrations from 10<sup>5</sup> to 10<sup>8</sup> and showed optimal disease control at 10<sup>7</sup> and 10<sup>8</sup> PFU/mL. Finally, under field conditions, both phage Φ31 alone and the phage cocktail treatments suppressed disease symptoms, which was comparable to the chemical bactericide oxolinic acid (Starner). Phage treatment also significantly improved yield, showing the potential of phage as a biocontrol strategy for managing leaf blight in Welsh onion.https://www.mdpi.com/2079-6382/10/5/517bacterial leaf blightbacteriophagesWelsh onion<i>Xanthomonas axonopodis</i> pv. <i>allii</i>biocontrol |
| spellingShingle | Nguyen Thi Thu Nga Tran Ngoc Tran Dominique Holtappels Nguyen Le Kim Ngan Nguyen Phuoc Hao Marta Vallino Doan Thi Kieu Tien Nguyen Huan Khanh-Pham Rob Lavigne Kaeko Kamei Jeroen Wagemans Jeffrey B. Jones Phage Biocontrol of Bacterial Leaf Blight Disease on Welsh Onion Caused by <i>Xanthomonas axonopodis</i> pv. <i>allii</i> Antibiotics bacterial leaf blight bacteriophages Welsh onion <i>Xanthomonas axonopodis</i> pv. <i>allii</i> biocontrol |
| title | Phage Biocontrol of Bacterial Leaf Blight Disease on Welsh Onion Caused by <i>Xanthomonas axonopodis</i> pv. <i>allii</i> |
| title_full | Phage Biocontrol of Bacterial Leaf Blight Disease on Welsh Onion Caused by <i>Xanthomonas axonopodis</i> pv. <i>allii</i> |
| title_fullStr | Phage Biocontrol of Bacterial Leaf Blight Disease on Welsh Onion Caused by <i>Xanthomonas axonopodis</i> pv. <i>allii</i> |
| title_full_unstemmed | Phage Biocontrol of Bacterial Leaf Blight Disease on Welsh Onion Caused by <i>Xanthomonas axonopodis</i> pv. <i>allii</i> |
| title_short | Phage Biocontrol of Bacterial Leaf Blight Disease on Welsh Onion Caused by <i>Xanthomonas axonopodis</i> pv. <i>allii</i> |
| title_sort | phage biocontrol of bacterial leaf blight disease on welsh onion caused by i xanthomonas axonopodis i pv i allii i |
| topic | bacterial leaf blight bacteriophages Welsh onion <i>Xanthomonas axonopodis</i> pv. <i>allii</i> biocontrol |
| url | https://www.mdpi.com/2079-6382/10/5/517 |
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