Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java

The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RF...

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Main Authors: Widya Pintaka Bayu Putra, Saiful Anwar, Syahruddin Said, Romanos Albert Adhitya Indarto, Putri Wulandari
Format: Article
Language:English
Published: Universitas Gadjah Mada 2019-02-01
Series:Buletin Peternakan
Subjects:
Online Access:https://jurnal.ugm.ac.id/buletinpeternakan/article/view/38227
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author Widya Pintaka Bayu Putra
Saiful Anwar
Syahruddin Said
Romanos Albert Adhitya Indarto
Putri Wulandari
author_facet Widya Pintaka Bayu Putra
Saiful Anwar
Syahruddin Said
Romanos Albert Adhitya Indarto
Putri Wulandari
author_sort Widya Pintaka Bayu Putra
collection DOAJ
description The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RFLP method. Research shows that TG gene of Pasundan cattle is monomorphic with C allele as the dominant allele (1.00). However, LEP gene of Pasundan cattle is polymorphic with C allele as the dominant allele (0.98) and T as the rare allele (0.02). The polymorphic informative content (PIC) and numberof effective allele (ne) values in the LEP gene in the animal studied were 0.04 and 1.04 respectively. It was concluded that TG/BstYI and LEP/Sau3AI gene in the present study can not be used as molecular selection in Pasundan cattle. These results are important as the basic information for preparing the molecular selection program in the future.
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spelling doaj.art-a093e9f004e943a9bfe26bd08b3f88a22022-12-21T19:23:33ZengUniversitas Gadjah MadaBuletin Peternakan0126-44002407-876X2019-02-0143110.21059/buletinpeternak.v43i1.3822723301Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West JavaWidya Pintaka Bayu Putra0Saiful Anwar1Syahruddin Said2Romanos Albert Adhitya Indarto3Putri Wulandari4Research Center for Biotechnology - Indonesian Institute of Sciences, West Java, 16911, IndonesiaResearch Center for Biotechnology - Indonesian Institute of Sciences, West Java, 16911, IndonesiaResearch Center for Biotechnology - Indonesian Institute of Sciences, West Java, 16911, IndonesiaDepartment of Biology, Faculty of Life Science, Surya University, Banten, 15810, IndonesiaDepartment of Biology, Faculty of Science and Technology, Jambi University, Jambi, 36361, IndonesiaThe Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RFLP method. Research shows that TG gene of Pasundan cattle is monomorphic with C allele as the dominant allele (1.00). However, LEP gene of Pasundan cattle is polymorphic with C allele as the dominant allele (0.98) and T as the rare allele (0.02). The polymorphic informative content (PIC) and numberof effective allele (ne) values in the LEP gene in the animal studied were 0.04 and 1.04 respectively. It was concluded that TG/BstYI and LEP/Sau3AI gene in the present study can not be used as molecular selection in Pasundan cattle. These results are important as the basic information for preparing the molecular selection program in the future.https://jurnal.ugm.ac.id/buletinpeternakan/article/view/38227leptinpasundan cattlepcr-rflpthyroglobulin
spellingShingle Widya Pintaka Bayu Putra
Saiful Anwar
Syahruddin Said
Romanos Albert Adhitya Indarto
Putri Wulandari
Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java
Buletin Peternakan
leptin
pasundan cattle
pcr-rflp
thyroglobulin
title Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java
title_full Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java
title_fullStr Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java
title_full_unstemmed Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java
title_short Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java
title_sort genetic characterization of thyroglobulin and leptin genes in pasundan cattle at west java
topic leptin
pasundan cattle
pcr-rflp
thyroglobulin
url https://jurnal.ugm.ac.id/buletinpeternakan/article/view/38227
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AT saifulanwar geneticcharacterizationofthyroglobulinandleptingenesinpasundancattleatwestjava
AT syahruddinsaid geneticcharacterizationofthyroglobulinandleptingenesinpasundancattleatwestjava
AT romanosalbertadhityaindarto geneticcharacterizationofthyroglobulinandleptingenesinpasundancattleatwestjava
AT putriwulandari geneticcharacterizationofthyroglobulinandleptingenesinpasundancattleatwestjava