Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones

Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones

Abstract Background CRISPR/Cas9 editing systems are currently used to generate mutations in a particular gene to mimic a genetic disorder in vitro. Such “disease in a dish” models based on human pluripotent stem cells (hPSCs) offer the opportunity to have access to virtually all cell types of the hu...

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Bibliographic Details
Main Authors: Elie Frank, Michel Cailleret, Constantin Nelep, Pascal Fragner, Jérome Polentes, Elise Herardot, Lina El Kassar, Karine Giraud-Triboult, Christelle Monville, Karim Ben M’Barek
Format: Article
Language:English
Published: BMC 2023-04-01
Series:Stem Cell Research & Therapy
Subjects:
CRISPR/Cas9
Gene edition
Human pluripotent stem cell
Clonal isolation
Online Access:https://doi.org/10.1186/s13287-023-03327-2

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