Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice
Abstract Background A possible relationship between periodontitis and Alzheimer’s disease (AD) has been reported. However, there is limited information on the association between the Porphyromonas gingivalis (P. gingivalis) periodontal infection and the pathological features of AD. The hypothesis th...
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | Immunity & Ageing |
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| Online Access: | http://link.springer.com/article/10.1186/s12979-017-0110-7 |
| _version_ | 1818321967444393984 |
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| author | Ye Ding Jingyi Ren Hongqiang Yu Weixian Yu Yanmin Zhou |
| author_facet | Ye Ding Jingyi Ren Hongqiang Yu Weixian Yu Yanmin Zhou |
| author_sort | Ye Ding |
| collection | DOAJ |
| description | Abstract Background A possible relationship between periodontitis and Alzheimer’s disease (AD) has been reported. However, there is limited information on the association between the Porphyromonas gingivalis (P. gingivalis) periodontal infection and the pathological features of AD. The hypothesis that P. gingivalis periodontal infection may cause cognitive impairment via age-dependent neuroinflammation was tested. Results Thirty 4-week-old (young) female C57BL/6 J mice were randomly divided into two groups, the control group and the experimental group. Thirty 12-month-old (middle-aged) were grouped as above. The mouth of the mice in the experimental group was infected with P. gingivalis. Morris water maze(MWM) was performed to assess the learning and memory ability of mice after 6 weeks. Moreover, the expression levels of the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β in the mice brain tissues were determined by Quantitative real-time polymerase chain reaction (qRT-PCR), Enzyme Linked Immunosorbent Assay(ELISA) and immunohistochemistry. Our results showed that the learning and memory abilities of the middle-aged P. gingivalis infected mice were impaired. Moreover, the expression levels of the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β in the brain tissues of the middle-aged P. gingivalis infected mice were increased. Conclusions These results suggest that P. gingivalis periodontal infection may cause cognitive impairment via the release of the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β in the brain tissues of middle-aged mice. |
| first_indexed | 2024-12-13T10:49:19Z |
| format | Article |
| id | doaj.art-a0978c7383ec41d995bc4cb27d178796 |
| institution | Directory Open Access Journal |
| issn | 1742-4933 |
| language | English |
| last_indexed | 2024-12-13T10:49:19Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Immunity & Ageing |
| spelling | doaj.art-a0978c7383ec41d995bc4cb27d1787962022-12-21T23:49:57ZengBMCImmunity & Ageing1742-49332018-01-011511810.1186/s12979-017-0110-7Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in miceYe Ding0Jingyi Ren1Hongqiang Yu2Weixian Yu3Yanmin Zhou4Department of Implantology, School and Hospital of Stomatology, Jilin UniversityDepartment of Implantology, School and Hospital of Stomatology, Jilin UniversityDepartment of Implantology, School and Hospital of Stomatology, Jilin UniversityKey laboratory of Mechanism of Tooth Development and Jaw Bone Remodeling and Regeneration in Jilin ProvinceDepartment of Implantology, School and Hospital of Stomatology, Jilin UniversityAbstract Background A possible relationship between periodontitis and Alzheimer’s disease (AD) has been reported. However, there is limited information on the association between the Porphyromonas gingivalis (P. gingivalis) periodontal infection and the pathological features of AD. The hypothesis that P. gingivalis periodontal infection may cause cognitive impairment via age-dependent neuroinflammation was tested. Results Thirty 4-week-old (young) female C57BL/6 J mice were randomly divided into two groups, the control group and the experimental group. Thirty 12-month-old (middle-aged) were grouped as above. The mouth of the mice in the experimental group was infected with P. gingivalis. Morris water maze(MWM) was performed to assess the learning and memory ability of mice after 6 weeks. Moreover, the expression levels of the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β in the mice brain tissues were determined by Quantitative real-time polymerase chain reaction (qRT-PCR), Enzyme Linked Immunosorbent Assay(ELISA) and immunohistochemistry. Our results showed that the learning and memory abilities of the middle-aged P. gingivalis infected mice were impaired. Moreover, the expression levels of the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β in the brain tissues of the middle-aged P. gingivalis infected mice were increased. Conclusions These results suggest that P. gingivalis periodontal infection may cause cognitive impairment via the release of the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β in the brain tissues of middle-aged mice.http://link.springer.com/article/10.1186/s12979-017-0110-7Porphyromonas gingivalis (P. gingivalis)PeriodontitisCognitionNeuroinflammationAlzheimer’s disease(AD) |
| spellingShingle | Ye Ding Jingyi Ren Hongqiang Yu Weixian Yu Yanmin Zhou Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice Immunity & Ageing Porphyromonas gingivalis (P. gingivalis) Periodontitis Cognition Neuroinflammation Alzheimer’s disease(AD) |
| title | Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice |
| title_full | Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice |
| title_fullStr | Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice |
| title_full_unstemmed | Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice |
| title_short | Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice |
| title_sort | porphyromonas gingivalis a periodontitis causing bacterium induces memory impairment and age dependent neuroinflammation in mice |
| topic | Porphyromonas gingivalis (P. gingivalis) Periodontitis Cognition Neuroinflammation Alzheimer’s disease(AD) |
| url | http://link.springer.com/article/10.1186/s12979-017-0110-7 |
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