Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering
Existing in vivo mutagenesis tools are limited by low mutation diversity and mutation rates. Here the authors present TRIDENT for targeted, continual and inducible diversification of genes of interest using deaminases fused to T7 RNA polymerase.
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-21876-z |
| _version_ | 1818749337438519296 |
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| author | Aaron Cravens Osman K. Jamil Deze Kong Jonathan T. Sockolosky Christina D. Smolke |
| author_facet | Aaron Cravens Osman K. Jamil Deze Kong Jonathan T. Sockolosky Christina D. Smolke |
| author_sort | Aaron Cravens |
| collection | DOAJ |
| description | Existing in vivo mutagenesis tools are limited by low mutation diversity and mutation rates. Here the authors present TRIDENT for targeted, continual and inducible diversification of genes of interest using deaminases fused to T7 RNA polymerase. |
| first_indexed | 2024-12-18T04:02:11Z |
| format | Article |
| id | doaj.art-a09839f69fe344c5b0b778bd2bc12f32 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-18T04:02:11Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-a09839f69fe344c5b0b778bd2bc12f322022-12-21T21:21:40ZengNature PortfolioNature Communications2041-17232021-03-0112111210.1038/s41467-021-21876-zPolymerase-guided base editing enables in vivo mutagenesis and rapid protein engineeringAaron Cravens0Osman K. Jamil1Deze Kong2Jonathan T. Sockolosky3Christina D. Smolke4Department of Bioengineering, 443 Via Ortega, MC 4245, Stanford UniversityDepartment of Chemical Engineering, 443 Via Ortega, MC 4245, Stanford UniversityDepartment of Bioengineering, 443 Via Ortega, MC 4245, Stanford UniversityDepartments of Molecular and Cellular Physiology and Structural Biology, Stanford University School of MedicineDepartment of Bioengineering, 443 Via Ortega, MC 4245, Stanford UniversityExisting in vivo mutagenesis tools are limited by low mutation diversity and mutation rates. Here the authors present TRIDENT for targeted, continual and inducible diversification of genes of interest using deaminases fused to T7 RNA polymerase.https://doi.org/10.1038/s41467-021-21876-z |
| spellingShingle | Aaron Cravens Osman K. Jamil Deze Kong Jonathan T. Sockolosky Christina D. Smolke Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering Nature Communications |
| title | Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering |
| title_full | Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering |
| title_fullStr | Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering |
| title_full_unstemmed | Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering |
| title_short | Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering |
| title_sort | polymerase guided base editing enables in vivo mutagenesis and rapid protein engineering |
| url | https://doi.org/10.1038/s41467-021-21876-z |
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