Metagenomic analysis of the salivary microbiota in patients with caries, periodontitis and comorbid diseases
Background/purpose: Previous studies have suggested that there is a mutual antagonism between caries and periodontitis. This research aimed to investigate the ecological connection and bacterial interaction of these two diseases. Materials and methods: We profiled and analyzed the salivary microbiot...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Journal of Dental Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790220302543 |
| _version_ | 1819114693360353280 |
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| author | Cong Shi Liting Cai Zhe Xun Saiwei Zheng Fangyang Shao Bingjie Wang Ruixin Zhu Yuan He |
| author_facet | Cong Shi Liting Cai Zhe Xun Saiwei Zheng Fangyang Shao Bingjie Wang Ruixin Zhu Yuan He |
| author_sort | Cong Shi |
| collection | DOAJ |
| description | Background/purpose: Previous studies have suggested that there is a mutual antagonism between caries and periodontitis. This research aimed to investigate the ecological connection and bacterial interaction of these two diseases. Materials and methods: We profiled and analyzed the salivary microbiota from 124 individuals (including 38 caries patients, 34 periodontitis patients, 15 comorbid diseases patients, and 37 healthy controls) by using 16 S rRNA gene sequencing and bioinformatics approaches, and also quantified their salivary bacteria loads via quantitative real-time PCR. The putative biological functions of the salivary microbiome of the different groups were predicted by PICRUSt. Results: We observed that both the total bacteria loads and the overall microbial richness in the saliva of the periodontitis group were higher than that in the healthy group. The principal coordinate analysis (PCoA) showed that the caries, periodontitis and healthy groups were separated from each other, and that the samples from comorbid diseases were located at the overlap of caries and periodontitis groups. Using LEfSe analysis, 20 differentially abundant genera were identified as potential biomarkers. These genera also performed complicated interactions among the four groups. Additionally, the PICRUSt analysis indicated caries-related and periodontitis-related functions (e.g., carbohydrate metabolism and bacteria proliferation) respectively. Conclusion: We disclosed the significant differences in the salivary bacterial community under caries, periodontitis and comorbid diseases. The periodontitis group was marked by the increased complexity of the salivary microbiota. The result may have vital clinical significance to the screening and early treatment of caries-active and periodontitis-active individuals. |
| first_indexed | 2024-12-22T04:49:21Z |
| format | Article |
| id | doaj.art-a0995f67199948aab418e42b7cc62a93 |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-22T04:49:21Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-a0995f67199948aab418e42b7cc62a932022-12-21T18:38:31ZengElsevierJournal of Dental Sciences1991-79022021-10-0116412641273Metagenomic analysis of the salivary microbiota in patients with caries, periodontitis and comorbid diseasesCong Shi0Liting Cai1Zhe Xun2Saiwei Zheng3Fangyang Shao4Bingjie Wang5Ruixin Zhu6Yuan He7Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School of Stomatology, Tongji University, Shanghai, China; Shanghai Jing-an Dental Clinic, Shanghai, ChinaDepartment of Bioinformatics, School of Life Sciences and Technology, Tongji University, Shanghai, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, School of Stomatology, Tongji University, Shanghai, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, School of Stomatology, Tongji University, Shanghai, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, School of Stomatology, Tongji University, Shanghai, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, School of Stomatology, Tongji University, Shanghai, ChinaDepartment of Bioinformatics, School of Life Sciences and Technology, Tongji University, Shanghai, China; Corresponding author. Department of Bioinformatics, School of life Sciences and technology, Tongji University, 1239 Siping Road, Shanghai, 200092, China.Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School of Stomatology, Tongji University, Shanghai, China; Corresponding author. Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School of Stomatology, Tongji University, 399 Middle Yanchang Road, Shanghai, 200072, China.Background/purpose: Previous studies have suggested that there is a mutual antagonism between caries and periodontitis. This research aimed to investigate the ecological connection and bacterial interaction of these two diseases. Materials and methods: We profiled and analyzed the salivary microbiota from 124 individuals (including 38 caries patients, 34 periodontitis patients, 15 comorbid diseases patients, and 37 healthy controls) by using 16 S rRNA gene sequencing and bioinformatics approaches, and also quantified their salivary bacteria loads via quantitative real-time PCR. The putative biological functions of the salivary microbiome of the different groups were predicted by PICRUSt. Results: We observed that both the total bacteria loads and the overall microbial richness in the saliva of the periodontitis group were higher than that in the healthy group. The principal coordinate analysis (PCoA) showed that the caries, periodontitis and healthy groups were separated from each other, and that the samples from comorbid diseases were located at the overlap of caries and periodontitis groups. Using LEfSe analysis, 20 differentially abundant genera were identified as potential biomarkers. These genera also performed complicated interactions among the four groups. Additionally, the PICRUSt analysis indicated caries-related and periodontitis-related functions (e.g., carbohydrate metabolism and bacteria proliferation) respectively. Conclusion: We disclosed the significant differences in the salivary bacterial community under caries, periodontitis and comorbid diseases. The periodontitis group was marked by the increased complexity of the salivary microbiota. The result may have vital clinical significance to the screening and early treatment of caries-active and periodontitis-active individuals.http://www.sciencedirect.com/science/article/pii/S1991790220302543MicrobiotaSaliva16S rRNA geneDental cariesPeriodontitis |
| spellingShingle | Cong Shi Liting Cai Zhe Xun Saiwei Zheng Fangyang Shao Bingjie Wang Ruixin Zhu Yuan He Metagenomic analysis of the salivary microbiota in patients with caries, periodontitis and comorbid diseases Journal of Dental Sciences Microbiota Saliva 16S rRNA gene Dental caries Periodontitis |
| title | Metagenomic analysis of the salivary microbiota in patients with caries, periodontitis and comorbid diseases |
| title_full | Metagenomic analysis of the salivary microbiota in patients with caries, periodontitis and comorbid diseases |
| title_fullStr | Metagenomic analysis of the salivary microbiota in patients with caries, periodontitis and comorbid diseases |
| title_full_unstemmed | Metagenomic analysis of the salivary microbiota in patients with caries, periodontitis and comorbid diseases |
| title_short | Metagenomic analysis of the salivary microbiota in patients with caries, periodontitis and comorbid diseases |
| title_sort | metagenomic analysis of the salivary microbiota in patients with caries periodontitis and comorbid diseases |
| topic | Microbiota Saliva 16S rRNA gene Dental caries Periodontitis |
| url | http://www.sciencedirect.com/science/article/pii/S1991790220302543 |
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