Gut Bacterial Diversity of Field and Laboratory-Reared <i>Aedes albopictus</i> Populations of Rio de Janeiro, Brazil
Background: The mosquito microbiota impacts different parameters in host biology, such as development, metabolism, immune response and vector competence to pathogens. As the environment is an important source of acquisition of host associate microbes, we described the microbiota and the vector compe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/6/1309 |
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| author | João M. C. Baltar Márcio G. Pavan Jessica Corrêa-Antônio Dinair Couto-Lima Rafael Maciel-de-Freitas Mariana R. David |
| author_facet | João M. C. Baltar Márcio G. Pavan Jessica Corrêa-Antônio Dinair Couto-Lima Rafael Maciel-de-Freitas Mariana R. David |
| author_sort | João M. C. Baltar |
| collection | DOAJ |
| description | Background: The mosquito microbiota impacts different parameters in host biology, such as development, metabolism, immune response and vector competence to pathogens. As the environment is an important source of acquisition of host associate microbes, we described the microbiota and the vector competence to Zika virus (ZIKV) of <i>Aedes albopictus</i> from three areas with distinct landscapes. Methods: Adult females were collected during two different seasons, while eggs were used to rear F1 colonies. Midgut bacterial communities were described in field and F1 mosquitoes as well as in insects from a laboratory colony (>30 generations, LAB) using 16S rRNA gene sequencing. F1 mosquitoes were infected with ZIKV to determine virus infection rates (IRs) and dissemination rates (DRs). Collection season significantly affected the bacterial microbiota diversity and composition, e.g., diversity levels decreased from the wet to the dry season. Field-collected and LAB mosquitoes’ microbiota had similar diversity levels, which were higher compared to F1 mosquitoes. However, the gut microbiota composition of field mosquitoes was distinct from that of laboratory-reared mosquitoes (LAB and F1), regardless of the collection season and location. A possible negative correlation was detected between Acetobacteraceae and <i>Wolbachia</i>, with the former dominating the gut microbiota of F1 <i>Ae. albopictus</i>, while the latter was absent/undetectable. Furthermore, we detected significant differences in infection and dissemination rates (but not in the viral load) between the mosquito populations, but it does not seem to be related to gut microbiota composition, as it was similar between F1 mosquitoes regardless of their population. Conclusions: Our results indicate that the environment and the collection season play a significant role in shaping mosquitoes’ bacterial microbiota. |
| first_indexed | 2024-03-11T01:50:17Z |
| format | Article |
| id | doaj.art-a099c33c53e945048b06988634d47854 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T01:50:17Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a099c33c53e945048b06988634d478542023-11-18T13:01:57ZengMDPI AGViruses1999-49152023-05-01156130910.3390/v15061309Gut Bacterial Diversity of Field and Laboratory-Reared <i>Aedes albopictus</i> Populations of Rio de Janeiro, BrazilJoão M. C. Baltar0Márcio G. Pavan1Jessica Corrêa-Antônio2Dinair Couto-Lima3Rafael Maciel-de-Freitas4Mariana R. David5Laboratório de Mosquitos Transmissores de Hematozoários, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, RJ, BrazilLaboratório de Mosquitos Transmissores de Hematozoários, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, RJ, BrazilLaboratório de Mosquitos Transmissores de Hematozoários, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, RJ, BrazilLaboratório de Mosquitos Transmissores de Hematozoários, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, RJ, BrazilLaboratório de Mosquitos Transmissores de Hematozoários, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, RJ, BrazilLaboratório de Mosquitos Transmissores de Hematozoários, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, RJ, BrazilBackground: The mosquito microbiota impacts different parameters in host biology, such as development, metabolism, immune response and vector competence to pathogens. As the environment is an important source of acquisition of host associate microbes, we described the microbiota and the vector competence to Zika virus (ZIKV) of <i>Aedes albopictus</i> from three areas with distinct landscapes. Methods: Adult females were collected during two different seasons, while eggs were used to rear F1 colonies. Midgut bacterial communities were described in field and F1 mosquitoes as well as in insects from a laboratory colony (>30 generations, LAB) using 16S rRNA gene sequencing. F1 mosquitoes were infected with ZIKV to determine virus infection rates (IRs) and dissemination rates (DRs). Collection season significantly affected the bacterial microbiota diversity and composition, e.g., diversity levels decreased from the wet to the dry season. Field-collected and LAB mosquitoes’ microbiota had similar diversity levels, which were higher compared to F1 mosquitoes. However, the gut microbiota composition of field mosquitoes was distinct from that of laboratory-reared mosquitoes (LAB and F1), regardless of the collection season and location. A possible negative correlation was detected between Acetobacteraceae and <i>Wolbachia</i>, with the former dominating the gut microbiota of F1 <i>Ae. albopictus</i>, while the latter was absent/undetectable. Furthermore, we detected significant differences in infection and dissemination rates (but not in the viral load) between the mosquito populations, but it does not seem to be related to gut microbiota composition, as it was similar between F1 mosquitoes regardless of their population. Conclusions: Our results indicate that the environment and the collection season play a significant role in shaping mosquitoes’ bacterial microbiota.https://www.mdpi.com/1999-4915/15/6/1309<i>Ae. albopictus</i>gut microbiotabacteriaZika16S RNA gene sequencing |
| spellingShingle | João M. C. Baltar Márcio G. Pavan Jessica Corrêa-Antônio Dinair Couto-Lima Rafael Maciel-de-Freitas Mariana R. David Gut Bacterial Diversity of Field and Laboratory-Reared <i>Aedes albopictus</i> Populations of Rio de Janeiro, Brazil Viruses <i>Ae. albopictus</i> gut microbiota bacteria Zika 16S RNA gene sequencing |
| title | Gut Bacterial Diversity of Field and Laboratory-Reared <i>Aedes albopictus</i> Populations of Rio de Janeiro, Brazil |
| title_full | Gut Bacterial Diversity of Field and Laboratory-Reared <i>Aedes albopictus</i> Populations of Rio de Janeiro, Brazil |
| title_fullStr | Gut Bacterial Diversity of Field and Laboratory-Reared <i>Aedes albopictus</i> Populations of Rio de Janeiro, Brazil |
| title_full_unstemmed | Gut Bacterial Diversity of Field and Laboratory-Reared <i>Aedes albopictus</i> Populations of Rio de Janeiro, Brazil |
| title_short | Gut Bacterial Diversity of Field and Laboratory-Reared <i>Aedes albopictus</i> Populations of Rio de Janeiro, Brazil |
| title_sort | gut bacterial diversity of field and laboratory reared i aedes albopictus i populations of rio de janeiro brazil |
| topic | <i>Ae. albopictus</i> gut microbiota bacteria Zika 16S RNA gene sequencing |
| url | https://www.mdpi.com/1999-4915/15/6/1309 |
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