Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders.
Live-cell microscopy imaging typically involves the use of high-quality glass-bottom chambers that allow cell culture, gaseous buffer exchange and optical properties suitable for microscopy applications. However, commercial sources of these chambers can add significant annual costs to cell biology l...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0269345 |
| _version_ | 1811290155531632640 |
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| author | Siobhan Goss Carlos Barba Bazan Kaitlyn Neuman Christina Peng Nola Begeja Celeste Elisabeth Suart Ray Truant |
| author_facet | Siobhan Goss Carlos Barba Bazan Kaitlyn Neuman Christina Peng Nola Begeja Celeste Elisabeth Suart Ray Truant |
| author_sort | Siobhan Goss |
| collection | DOAJ |
| description | Live-cell microscopy imaging typically involves the use of high-quality glass-bottom chambers that allow cell culture, gaseous buffer exchange and optical properties suitable for microscopy applications. However, commercial sources of these chambers can add significant annual costs to cell biology laboratories. Consumer products in three-dimensional printing technology, for both Filament Deposition Modeling (FDM) and Masked Stereo Lithography (MSLA), have resulted in more biomedical research labs adopting the use of these devices for prototyping and manufacturing of lab plastic-based items, but rarely consumables. Here we describe a modular, live-cell chamber with multiple design options that can be mixed per experiment. Single reusable carriers and the use of biodegradable plastics, in a hybrid of FDM and MSLA manufacturing methods, reduce plastic waste. The system is easy to adapt to bespoke designs, with concept-to-prototype in a single day, offers significant cost savings to the users over commercial sources, and no loss in dimensional quality or reliability. |
| first_indexed | 2024-04-13T04:08:01Z |
| format | Article |
| id | doaj.art-a09a40222be942cea9e3eb91ead6003a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T04:08:01Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a09a40222be942cea9e3eb91ead6003a2022-12-22T03:03:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01176e026934510.1371/journal.pone.0269345Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders.Siobhan GossCarlos Barba BazanKaitlyn NeumanChristina PengNola BegejaCeleste Elisabeth SuartRay TruantLive-cell microscopy imaging typically involves the use of high-quality glass-bottom chambers that allow cell culture, gaseous buffer exchange and optical properties suitable for microscopy applications. However, commercial sources of these chambers can add significant annual costs to cell biology laboratories. Consumer products in three-dimensional printing technology, for both Filament Deposition Modeling (FDM) and Masked Stereo Lithography (MSLA), have resulted in more biomedical research labs adopting the use of these devices for prototyping and manufacturing of lab plastic-based items, but rarely consumables. Here we describe a modular, live-cell chamber with multiple design options that can be mixed per experiment. Single reusable carriers and the use of biodegradable plastics, in a hybrid of FDM and MSLA manufacturing methods, reduce plastic waste. The system is easy to adapt to bespoke designs, with concept-to-prototype in a single day, offers significant cost savings to the users over commercial sources, and no loss in dimensional quality or reliability.https://doi.org/10.1371/journal.pone.0269345 |
| spellingShingle | Siobhan Goss Carlos Barba Bazan Kaitlyn Neuman Christina Peng Nola Begeja Celeste Elisabeth Suart Ray Truant Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders. PLoS ONE |
| title | Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders. |
| title_full | Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders. |
| title_fullStr | Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders. |
| title_full_unstemmed | Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders. |
| title_short | Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders. |
| title_sort | mod3d a low cost flexible modular system of live cell microscopy chambers and holders |
| url | https://doi.org/10.1371/journal.pone.0269345 |
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