Modularity of RBC hitchhiking with polymeric nanoparticles: testing the limits of non-covalent adsorption
Abstract Red blood cell (RBC) hitchhiking has great potential in enhancing drug therapy, by improving targeting and reducing rapid clearance of nanoparticles (NPs). However, to improve the potential for clinical translation of RBC hitchhiking, a more thorough understanding of the RBC-NP interface is...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-022-01544-0 |
| _version_ | 1811278306840936448 |
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| author | Vincent Lenders Remei Escudero Xanthippi Koutsoumpou Laura Armengol Álvarez Jef Rozenski Stefaan J. Soenen Zongmin Zhao Samir Mitragotri Pieter Baatsen Karel Allegaert Jaan Toelen Bella B. Manshian |
| author_facet | Vincent Lenders Remei Escudero Xanthippi Koutsoumpou Laura Armengol Álvarez Jef Rozenski Stefaan J. Soenen Zongmin Zhao Samir Mitragotri Pieter Baatsen Karel Allegaert Jaan Toelen Bella B. Manshian |
| author_sort | Vincent Lenders |
| collection | DOAJ |
| description | Abstract Red blood cell (RBC) hitchhiking has great potential in enhancing drug therapy, by improving targeting and reducing rapid clearance of nanoparticles (NPs). However, to improve the potential for clinical translation of RBC hitchhiking, a more thorough understanding of the RBC-NP interface is needed. Here, we evaluate the effects of NP surface parameters on the success and biocompatibility of NP adsorption to extracted RBCs from various species. Major differences in RBC characteristics between rabbit, mouse and human were proven to significantly impact NP adsorption outcomes. Additionally, the effects of NP design parameters, including NP hydrophobicity, zeta potential, surfactant concentration and drug encapsulation, on RBC hitchhiking are investigated. Our studies demonstrate the importance of electrostatic interactions in balancing NP adsorption success and biocompatibility. We further investigated the effect of varying the anti-coagulant used for blood storage. The results presented here offer new insights into the parameters that impact NP adsorption on RBCs that will assist researchers in experimental design choices for using RBC hitchhiking as drug delivery strategy. |
| first_indexed | 2024-04-13T00:33:40Z |
| format | Article |
| id | doaj.art-a09c6c905afe49e68fea09c18b477f58 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-13T00:33:40Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-a09c6c905afe49e68fea09c18b477f582022-12-22T03:10:24ZengBMCJournal of Nanobiotechnology1477-31552022-07-0120111210.1186/s12951-022-01544-0Modularity of RBC hitchhiking with polymeric nanoparticles: testing the limits of non-covalent adsorptionVincent Lenders0Remei Escudero1Xanthippi Koutsoumpou2Laura Armengol Álvarez3Jef Rozenski4Stefaan J. Soenen5Zongmin Zhao6Samir Mitragotri7Pieter Baatsen8Karel Allegaert9Jaan Toelen10Bella B. Manshian11Translational Cell and Tissue Research Unit, Department of Imaging and Pathology, KU LeuvenTranslational Cell and Tissue Research Unit, Department of Imaging and Pathology, KU LeuvenTranslational Cell and Tissue Research Unit, Department of Imaging and Pathology, KU LeuvenMedicinal Chemistry, Rega Institute for Medical Research, Department of Pharmaceutical and Pharmacological Sciences, KU LeuvenMedicinal Chemistry, Rega Institute for Medical Research, Department of Pharmaceutical and Pharmacological Sciences, KU LeuvenTranslational Cell and Tissue Research Unit, Department of Imaging and Pathology, KU LeuvenDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois at ChicagoJohn A. Paulson School of Engineering and Applied Sciences, Harvard UniversityVIB-KU Leuven Center for Brain and Disease Research Electron Microscopy Platform of the VIB Bioimaging CoreDepartment of Hospital Pharmacy, Erasmus MC University Medical CenterLeuven Child and Youth Institute, KU LeuvenTranslational Cell and Tissue Research Unit, Department of Imaging and Pathology, KU LeuvenAbstract Red blood cell (RBC) hitchhiking has great potential in enhancing drug therapy, by improving targeting and reducing rapid clearance of nanoparticles (NPs). However, to improve the potential for clinical translation of RBC hitchhiking, a more thorough understanding of the RBC-NP interface is needed. Here, we evaluate the effects of NP surface parameters on the success and biocompatibility of NP adsorption to extracted RBCs from various species. Major differences in RBC characteristics between rabbit, mouse and human were proven to significantly impact NP adsorption outcomes. Additionally, the effects of NP design parameters, including NP hydrophobicity, zeta potential, surfactant concentration and drug encapsulation, on RBC hitchhiking are investigated. Our studies demonstrate the importance of electrostatic interactions in balancing NP adsorption success and biocompatibility. We further investigated the effect of varying the anti-coagulant used for blood storage. The results presented here offer new insights into the parameters that impact NP adsorption on RBCs that will assist researchers in experimental design choices for using RBC hitchhiking as drug delivery strategy.https://doi.org/10.1186/s12951-022-01544-0Drug deliveryPolymeric nanoparticlesRed blood cellsNon-covalent adsorptionSurface properties |
| spellingShingle | Vincent Lenders Remei Escudero Xanthippi Koutsoumpou Laura Armengol Álvarez Jef Rozenski Stefaan J. Soenen Zongmin Zhao Samir Mitragotri Pieter Baatsen Karel Allegaert Jaan Toelen Bella B. Manshian Modularity of RBC hitchhiking with polymeric nanoparticles: testing the limits of non-covalent adsorption Journal of Nanobiotechnology Drug delivery Polymeric nanoparticles Red blood cells Non-covalent adsorption Surface properties |
| title | Modularity of RBC hitchhiking with polymeric nanoparticles: testing the limits of non-covalent adsorption |
| title_full | Modularity of RBC hitchhiking with polymeric nanoparticles: testing the limits of non-covalent adsorption |
| title_fullStr | Modularity of RBC hitchhiking with polymeric nanoparticles: testing the limits of non-covalent adsorption |
| title_full_unstemmed | Modularity of RBC hitchhiking with polymeric nanoparticles: testing the limits of non-covalent adsorption |
| title_short | Modularity of RBC hitchhiking with polymeric nanoparticles: testing the limits of non-covalent adsorption |
| title_sort | modularity of rbc hitchhiking with polymeric nanoparticles testing the limits of non covalent adsorption |
| topic | Drug delivery Polymeric nanoparticles Red blood cells Non-covalent adsorption Surface properties |
| url | https://doi.org/10.1186/s12951-022-01544-0 |
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