Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis
Background Bone homeostasis is a tightly orchestrated process maintained by osteoblasts and osteoclasts, and a disruption of their steady-state equilibrium can lead to the occurrence of osteoporosis (OP). Methods We investigated the differential expression of micro (mi)RNAs in the bone tissues of a...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-12-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/0300060520978015 |
| _version_ | 1819290163212189696 |
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| author | Ruran Wang Yanhua Feng Huaying Xu Haoran Huang Shan Zhao Yuhong Wang Hongyan Li Jian Cao Guoying Xu Shengnan Huang |
| author_facet | Ruran Wang Yanhua Feng Huaying Xu Haoran Huang Shan Zhao Yuhong Wang Hongyan Li Jian Cao Guoying Xu Shengnan Huang |
| author_sort | Ruran Wang |
| collection | DOAJ |
| description | Background Bone homeostasis is a tightly orchestrated process maintained by osteoblasts and osteoclasts, and a disruption of their steady-state equilibrium can lead to the occurrence of osteoporosis (OP). Methods We investigated the differential expression of micro (mi)RNAs in the bone tissues of a postmenopausal osteoporosis rat model induced by ovariectomy (OVX). Real-time PCR was used to verify the differentially expressed miRNAs in bone samples from OP patients and controls. The specific targets of two differentially expressed miRNAs in osteogenic or osteoclast differentiation were determined by bioinformatic prediction, and mRNA and protein detection. Results miR-708-5p and miR-708-3p were highly expressed in the bone tissue of OVX rats and OP patients. miR-708-5p negatively regulated osteoblast differentiation in bone marrow mesenchymal stem cells by targeting SMAD specific E3 ubiquitin protein ligase 2, while miR-708-3p positively regulated osteoclast differentiation in bone marrow monocytes by targeting cerebellar degeneration associated protein 1 antisense RNA. miR-708-5p and miR-708-3p were shown to originate from the same precursor miRNA and to have a synergistic effect on the development of osteoporosis with different temporal and spatial patterns. Conclusion Our findings provide a referential theoretical basis and targets for the prevention and treatment of osteoporosis. |
| first_indexed | 2024-12-24T03:18:22Z |
| format | Article |
| id | doaj.art-a09e35f84a91424a98dcef8b5f36b84b |
| institution | Directory Open Access Journal |
| issn | 1473-2300 |
| language | English |
| last_indexed | 2024-12-24T03:18:22Z |
| publishDate | 2020-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-a09e35f84a91424a98dcef8b5f36b84b2022-12-21T17:17:34ZengSAGE PublishingJournal of International Medical Research1473-23002020-12-014810.1177/0300060520978015Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosisRuran WangYanhua FengHuaying XuHaoran HuangShan ZhaoYuhong WangHongyan LiJian CaoGuoying XuShengnan HuangBackground Bone homeostasis is a tightly orchestrated process maintained by osteoblasts and osteoclasts, and a disruption of their steady-state equilibrium can lead to the occurrence of osteoporosis (OP). Methods We investigated the differential expression of micro (mi)RNAs in the bone tissues of a postmenopausal osteoporosis rat model induced by ovariectomy (OVX). Real-time PCR was used to verify the differentially expressed miRNAs in bone samples from OP patients and controls. The specific targets of two differentially expressed miRNAs in osteogenic or osteoclast differentiation were determined by bioinformatic prediction, and mRNA and protein detection. Results miR-708-5p and miR-708-3p were highly expressed in the bone tissue of OVX rats and OP patients. miR-708-5p negatively regulated osteoblast differentiation in bone marrow mesenchymal stem cells by targeting SMAD specific E3 ubiquitin protein ligase 2, while miR-708-3p positively regulated osteoclast differentiation in bone marrow monocytes by targeting cerebellar degeneration associated protein 1 antisense RNA. miR-708-5p and miR-708-3p were shown to originate from the same precursor miRNA and to have a synergistic effect on the development of osteoporosis with different temporal and spatial patterns. Conclusion Our findings provide a referential theoretical basis and targets for the prevention and treatment of osteoporosis.https://doi.org/10.1177/0300060520978015 |
| spellingShingle | Ruran Wang Yanhua Feng Huaying Xu Haoran Huang Shan Zhao Yuhong Wang Hongyan Li Jian Cao Guoying Xu Shengnan Huang Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis Journal of International Medical Research |
| title | Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis |
| title_full | Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis |
| title_fullStr | Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis |
| title_full_unstemmed | Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis |
| title_short | Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis |
| title_sort | synergistic effects of mir 708 5p and mir 708 3p accelerate the progression of osteoporosis |
| url | https://doi.org/10.1177/0300060520978015 |
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