Structure of the Helicobacter pylori Cag type IV secretion system
Bacterial type IV secretion systems (T4SSs) are molecular machines that can mediate interbacterial DNA transfer through conjugation and delivery of effector molecules into host cells. The Helicobacter pylori Cag T4SS translocates CagA, a bacterial oncoprotein, into gastric cells, contributing to gas...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-06-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/47644 |
| _version_ | 1811201340687253504 |
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| author | Jeong Min Chung Michael J Sheedlo Anne M Campbell Neha Sawhney Arwen E Frick-Cheng Dana Borden Lacy Timothy L Cover Melanie D Ohi |
| author_facet | Jeong Min Chung Michael J Sheedlo Anne M Campbell Neha Sawhney Arwen E Frick-Cheng Dana Borden Lacy Timothy L Cover Melanie D Ohi |
| author_sort | Jeong Min Chung |
| collection | DOAJ |
| description | Bacterial type IV secretion systems (T4SSs) are molecular machines that can mediate interbacterial DNA transfer through conjugation and delivery of effector molecules into host cells. The Helicobacter pylori Cag T4SS translocates CagA, a bacterial oncoprotein, into gastric cells, contributing to gastric cancer pathogenesis. We report the structure of a membrane-spanning Cag T4SS assembly, which we describe as three sub-assemblies: a 14-fold symmetric outer membrane core complex (OMCC), 17-fold symmetric periplasmic ring complex (PRC), and central stalk. Features that differ markedly from those of prototypical T4SSs include an expanded OMCC and unexpected symmetry mismatch between the OMCC and PRC. This structure is one of the largest bacterial secretion system assemblies ever reported and illustrates the remarkable structural diversity that exists among bacterial T4SSs. |
| first_indexed | 2024-04-12T02:20:15Z |
| format | Article |
| id | doaj.art-a09e84dbd1fd47248cd182384fd9c270 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:20:15Z |
| publishDate | 2019-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a09e84dbd1fd47248cd182384fd9c2702022-12-22T03:52:09ZengeLife Sciences Publications LtdeLife2050-084X2019-06-01810.7554/eLife.47644Structure of the Helicobacter pylori Cag type IV secretion systemJeong Min Chung0https://orcid.org/0000-0002-4285-8764Michael J Sheedlo1https://orcid.org/0000-0002-3185-1727Anne M Campbell2Neha Sawhney3https://orcid.org/0000-0002-4943-1018Arwen E Frick-Cheng4Dana Borden Lacy5Timothy L Cover6https://orcid.org/0000-0001-8503-002XMelanie D Ohi7https://orcid.org/0000-0003-1750-4793Life Sciences Institute, University of Michigan, Ann Arbor, United StatesDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, United StatesDepartment of Medicine, Vanderbilt University School of Medicine, Nashville, United StatesDepartment of Medicine, Vanderbilt University School of Medicine, Nashville, United StatesDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, United States; Department of Immunology and Microbiology, University of Michigan School of Medicine, Ann Arbor, United StatesDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, United States; Veterans Affairs Tennessee Valley Healthcare System, Nashville, United StatesDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, United States; Department of Medicine, Vanderbilt University School of Medicine, Nashville, United States; Veterans Affairs Tennessee Valley Healthcare System, Nashville, United StatesLife Sciences Institute, University of Michigan, Ann Arbor, United States; Department of Cell and Developmental Biology, University of Michigan School of Medicine, Ann Arbor, United StatesBacterial type IV secretion systems (T4SSs) are molecular machines that can mediate interbacterial DNA transfer through conjugation and delivery of effector molecules into host cells. The Helicobacter pylori Cag T4SS translocates CagA, a bacterial oncoprotein, into gastric cells, contributing to gastric cancer pathogenesis. We report the structure of a membrane-spanning Cag T4SS assembly, which we describe as three sub-assemblies: a 14-fold symmetric outer membrane core complex (OMCC), 17-fold symmetric periplasmic ring complex (PRC), and central stalk. Features that differ markedly from those of prototypical T4SSs include an expanded OMCC and unexpected symmetry mismatch between the OMCC and PRC. This structure is one of the largest bacterial secretion system assemblies ever reported and illustrates the remarkable structural diversity that exists among bacterial T4SSs.https://elifesciences.org/articles/47644Helicobacter pyloriType IV secretion systemgastric cancercryo-electron microscopybacterial protein secretion |
| spellingShingle | Jeong Min Chung Michael J Sheedlo Anne M Campbell Neha Sawhney Arwen E Frick-Cheng Dana Borden Lacy Timothy L Cover Melanie D Ohi Structure of the Helicobacter pylori Cag type IV secretion system eLife Helicobacter pylori Type IV secretion system gastric cancer cryo-electron microscopy bacterial protein secretion |
| title | Structure of the Helicobacter pylori Cag type IV secretion system |
| title_full | Structure of the Helicobacter pylori Cag type IV secretion system |
| title_fullStr | Structure of the Helicobacter pylori Cag type IV secretion system |
| title_full_unstemmed | Structure of the Helicobacter pylori Cag type IV secretion system |
| title_short | Structure of the Helicobacter pylori Cag type IV secretion system |
| title_sort | structure of the helicobacter pylori cag type iv secretion system |
| topic | Helicobacter pylori Type IV secretion system gastric cancer cryo-electron microscopy bacterial protein secretion |
| url | https://elifesciences.org/articles/47644 |
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