Modulation of GABA by sodium butyrate ameliorates hypothalamic inflammation in experimental model of PCOS
Abstract Polycystic ovarian syndrome (PCOS) is a known endocrine disorder that has affected many women of childbearing age, and is accompanied by various neurodegenerative conditions. Hence, this study investigates the impact of butyrate in reversing hypothalamic-related disorder, possibly through γ...
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Neuroscience |
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| Online Access: | https://doi.org/10.1186/s12868-023-00834-z |
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| author | Oony-Iye Eepho Al-Amin M. Bashir Adesola A. Oniyide Ayodeji Aturamu Olutunmise V. Owolabi Isaac O. Ajadi Adedamola A. Fafure Mary B. Ajadi Stephanie E. Areloegbe Kehinde S. Olaniyi |
| author_facet | Oony-Iye Eepho Al-Amin M. Bashir Adesola A. Oniyide Ayodeji Aturamu Olutunmise V. Owolabi Isaac O. Ajadi Adedamola A. Fafure Mary B. Ajadi Stephanie E. Areloegbe Kehinde S. Olaniyi |
| author_sort | Oony-Iye Eepho |
| collection | DOAJ |
| description | Abstract Polycystic ovarian syndrome (PCOS) is a known endocrine disorder that has affected many women of childbearing age, and is accompanied by various neurodegenerative conditions. Hence, this study investigates the impact of butyrate in reversing hypothalamic-related disorder, possibly through γ aminobutyric acid (GABA) in a rat model of PCOS. Eight-week-old female Wistar rats were allotted into four groups (n = 5), which include control, butyrate, letrozole, and letrozole + butyrate groups. PCOS was induced by administering 1 mg/kg of letrozole (oral gavage) for 21 days. After confirmation of PCOS, 200 mg/kg of butyrate (oral gavage) was administered for 6 weeks. Rats with PCOS were characterized by elevated levels of plasma insulin and testosterone. Increases in plasma and hypothalamic triglyceride levels, inflammatory biomarker (SDF-1), apoptotic marker (caspase-6), and decreased plasma GnRH were observed. Additionally, a decrease in hypothalamic GABA was revealed. Nevertheless, the administration of butyrate attenuated these alterations. The present study suggests that butyrate ameliorates hypothalamic inflammation in an experimental model of PCOS, a beneficial effect that is accompanied by enhanced GABA production. |
| first_indexed | 2024-03-09T15:27:27Z |
| format | Article |
| id | doaj.art-a09e8a1cd92a4751a0f67b916f92cf7a |
| institution | Directory Open Access Journal |
| issn | 1471-2202 |
| language | English |
| last_indexed | 2024-03-09T15:27:27Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj.art-a09e8a1cd92a4751a0f67b916f92cf7a2023-11-26T12:26:45ZengBMCBMC Neuroscience1471-22022023-11-012411810.1186/s12868-023-00834-zModulation of GABA by sodium butyrate ameliorates hypothalamic inflammation in experimental model of PCOSOony-Iye Eepho0Al-Amin M. Bashir1Adesola A. Oniyide2Ayodeji Aturamu3Olutunmise V. Owolabi4Isaac O. Ajadi5Adedamola A. Fafure6Mary B. Ajadi7Stephanie E. Areloegbe8Kehinde S. Olaniyi9Cardio/Endo-metabolic and Microbiome Research Unit, Department of Physiology, College of Medicine and Health Sciences, Afe Babalola UniversityCardio/Endo-metabolic and Microbiome Research Unit, Department of Physiology, College of Medicine and Health Sciences, Afe Babalola UniversityCardio/Endo-metabolic and Microbiome Research Unit, Department of Physiology, College of Medicine and Health Sciences, Afe Babalola UniversityCardio/Endo-metabolic and Microbiome Research Unit, Department of Physiology, College of Medicine and Health Sciences, Afe Babalola UniversityDepartment of Biochemistry, College of Medicine and Health Sciences, Afe Babalola UniversityDepartment of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of TechnologyDepartment of Anatomy, College of Medicine and Health Sciences, Afe Babalola UniversityDepartment of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of TechnologyCardio/Endo-metabolic and Microbiome Research Unit, Department of Physiology, College of Medicine and Health Sciences, Afe Babalola UniversityCardio/Endo-metabolic and Microbiome Research Unit, Department of Physiology, College of Medicine and Health Sciences, Afe Babalola UniversityAbstract Polycystic ovarian syndrome (PCOS) is a known endocrine disorder that has affected many women of childbearing age, and is accompanied by various neurodegenerative conditions. Hence, this study investigates the impact of butyrate in reversing hypothalamic-related disorder, possibly through γ aminobutyric acid (GABA) in a rat model of PCOS. Eight-week-old female Wistar rats were allotted into four groups (n = 5), which include control, butyrate, letrozole, and letrozole + butyrate groups. PCOS was induced by administering 1 mg/kg of letrozole (oral gavage) for 21 days. After confirmation of PCOS, 200 mg/kg of butyrate (oral gavage) was administered for 6 weeks. Rats with PCOS were characterized by elevated levels of plasma insulin and testosterone. Increases in plasma and hypothalamic triglyceride levels, inflammatory biomarker (SDF-1), apoptotic marker (caspase-6), and decreased plasma GnRH were observed. Additionally, a decrease in hypothalamic GABA was revealed. Nevertheless, the administration of butyrate attenuated these alterations. The present study suggests that butyrate ameliorates hypothalamic inflammation in an experimental model of PCOS, a beneficial effect that is accompanied by enhanced GABA production.https://doi.org/10.1186/s12868-023-00834-zButyrateInflammationHypothalamusPCOSGABA |
| spellingShingle | Oony-Iye Eepho Al-Amin M. Bashir Adesola A. Oniyide Ayodeji Aturamu Olutunmise V. Owolabi Isaac O. Ajadi Adedamola A. Fafure Mary B. Ajadi Stephanie E. Areloegbe Kehinde S. Olaniyi Modulation of GABA by sodium butyrate ameliorates hypothalamic inflammation in experimental model of PCOS BMC Neuroscience Butyrate Inflammation Hypothalamus PCOS GABA |
| title | Modulation of GABA by sodium butyrate ameliorates hypothalamic inflammation in experimental model of PCOS |
| title_full | Modulation of GABA by sodium butyrate ameliorates hypothalamic inflammation in experimental model of PCOS |
| title_fullStr | Modulation of GABA by sodium butyrate ameliorates hypothalamic inflammation in experimental model of PCOS |
| title_full_unstemmed | Modulation of GABA by sodium butyrate ameliorates hypothalamic inflammation in experimental model of PCOS |
| title_short | Modulation of GABA by sodium butyrate ameliorates hypothalamic inflammation in experimental model of PCOS |
| title_sort | modulation of gaba by sodium butyrate ameliorates hypothalamic inflammation in experimental model of pcos |
| topic | Butyrate Inflammation Hypothalamus PCOS GABA |
| url | https://doi.org/10.1186/s12868-023-00834-z |
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