A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates Plant Height in Rice
The APETALA 2/ethylene response factors (AP2/ERF) are widespread in the plant kingdom and play essential roles in regulating plant growth and development as well as defense responses. In this study, a novel rice AP2/ERF transcription factor gene, OsRPH1, was isolated and functionally characterized....
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00709/full |
| _version_ | 1819158430504452096 |
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| author | Ziming Ma Tao Wu Kai Huang Yong-Mei Jin Zhao Li Mojun Chen Sokyong Yun Hongjia Zhang Xue Yang Haoyuan Chen Huijiao Bai Lin Du Shanshan Ju Liping Guo Mingdi Bian Lanjuan Hu Xinglin Du Wenzhu Jiang |
| author_facet | Ziming Ma Tao Wu Kai Huang Yong-Mei Jin Zhao Li Mojun Chen Sokyong Yun Hongjia Zhang Xue Yang Haoyuan Chen Huijiao Bai Lin Du Shanshan Ju Liping Guo Mingdi Bian Lanjuan Hu Xinglin Du Wenzhu Jiang |
| author_sort | Ziming Ma |
| collection | DOAJ |
| description | The APETALA 2/ethylene response factors (AP2/ERF) are widespread in the plant kingdom and play essential roles in regulating plant growth and development as well as defense responses. In this study, a novel rice AP2/ERF transcription factor gene, OsRPH1, was isolated and functionally characterized. OsRPH1 falls into group-IVa of the AP2/ERF family. OsRPH1 protein was found to be localized in the nucleus and possessed transcriptional activity. Overexpression of OsRPH1 resulted in a decrease in plant height and length of internode and leaf sheath as well as other abnormal characters in rice. The length of the second leaf sheath of OsRPH1-overexpressing (OE) plants recovered to that of Kitaake (non-transgenic recipient) in response to exogenous gibberellin A3 (GA3) application. The expression of GA biosynthesis genes (OsGA20ox1–OsGA20ox4, OsGA3ox1, and OsGA3ox2) was significantly downregulated, whereas that of GA inactivation genes (OsGA2ox7, OsGA2ox9, and OsGA2ox10) was significantly upregulated in OsRPH1-OE plants. Endogenous bioactive GA contents significantly decreased in OsRPH1-OE plants. OsRPH1 interacted with a blue light receptor, OsCRY1b, in a blue light-dependent manner. Taken together, our results demonstrate that OsRPH1 negatively regulates plant height and bioactive GA content by controlling the expression of GA metabolism genes in rice. OsRPH1 is involved in blue light inhibition of leaf sheath elongation by interacting with OsCRY1b. |
| first_indexed | 2024-12-22T16:24:32Z |
| format | Article |
| id | doaj.art-a0a40e28e3ad4223bbb4c63f4f8a10c1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T16:24:32Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a0a40e28e3ad4223bbb4c63f4f8a10c12022-12-21T18:20:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-05-011110.3389/fpls.2020.00709528430A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates Plant Height in RiceZiming Ma0Tao Wu1Kai Huang2Yong-Mei Jin3Zhao Li4Mojun Chen5Sokyong Yun6Hongjia Zhang7Xue Yang8Haoyuan Chen9Huijiao Bai10Lin Du11Shanshan Ju12Liping Guo13Mingdi Bian14Lanjuan Hu15Xinglin Du16Wenzhu Jiang17Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Academy of Agricultural Sciences, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Academy of Agricultural Sciences, Changchun, ChinaKye Ung Sang College of Agriculture of Kim II Sung University, Pyongyang, North KoreaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun, ChinaThe APETALA 2/ethylene response factors (AP2/ERF) are widespread in the plant kingdom and play essential roles in regulating plant growth and development as well as defense responses. In this study, a novel rice AP2/ERF transcription factor gene, OsRPH1, was isolated and functionally characterized. OsRPH1 falls into group-IVa of the AP2/ERF family. OsRPH1 protein was found to be localized in the nucleus and possessed transcriptional activity. Overexpression of OsRPH1 resulted in a decrease in plant height and length of internode and leaf sheath as well as other abnormal characters in rice. The length of the second leaf sheath of OsRPH1-overexpressing (OE) plants recovered to that of Kitaake (non-transgenic recipient) in response to exogenous gibberellin A3 (GA3) application. The expression of GA biosynthesis genes (OsGA20ox1–OsGA20ox4, OsGA3ox1, and OsGA3ox2) was significantly downregulated, whereas that of GA inactivation genes (OsGA2ox7, OsGA2ox9, and OsGA2ox10) was significantly upregulated in OsRPH1-OE plants. Endogenous bioactive GA contents significantly decreased in OsRPH1-OE plants. OsRPH1 interacted with a blue light receptor, OsCRY1b, in a blue light-dependent manner. Taken together, our results demonstrate that OsRPH1 negatively regulates plant height and bioactive GA content by controlling the expression of GA metabolism genes in rice. OsRPH1 is involved in blue light inhibition of leaf sheath elongation by interacting with OsCRY1b.https://www.frontiersin.org/article/10.3389/fpls.2020.00709/fullAP2/ERF transcription factorOsPRH1plant heightOsCRY1brice |
| spellingShingle | Ziming Ma Tao Wu Kai Huang Yong-Mei Jin Zhao Li Mojun Chen Sokyong Yun Hongjia Zhang Xue Yang Haoyuan Chen Huijiao Bai Lin Du Shanshan Ju Liping Guo Mingdi Bian Lanjuan Hu Xinglin Du Wenzhu Jiang A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates Plant Height in Rice Frontiers in Plant Science AP2/ERF transcription factor OsPRH1 plant height OsCRY1b rice |
| title | A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates Plant Height in Rice |
| title_full | A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates Plant Height in Rice |
| title_fullStr | A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates Plant Height in Rice |
| title_full_unstemmed | A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates Plant Height in Rice |
| title_short | A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates Plant Height in Rice |
| title_sort | novel ap2 erf transcription factor osrph1 negatively regulates plant height in rice |
| topic | AP2/ERF transcription factor OsPRH1 plant height OsCRY1b rice |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00709/full |
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