l-Theanine improves functional recovery after traumatic spinal cord injury in rats
Background/Purpose: Spinal cord injury (SCI) is a devastating medical condition for which no effective pharmacological interventions exist. l-Theanine (LT), a major amino acid component of green tea, exhibits potent antioxidative and anti-inflammatory activities and protects against various neural i...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664619306047 |
| _version_ | 1818506280964194304 |
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| author | Chih-Chuan Yang Kuo-Chi Chang Mao-Hsien Wang Hsiang-Chien Tseng Hung-Sheng Soung Chih-Hsiang Fang Yi-Wen Lin Keng-Yuan Li Cheng-Chia Tsai |
| author_facet | Chih-Chuan Yang Kuo-Chi Chang Mao-Hsien Wang Hsiang-Chien Tseng Hung-Sheng Soung Chih-Hsiang Fang Yi-Wen Lin Keng-Yuan Li Cheng-Chia Tsai |
| author_sort | Chih-Chuan Yang |
| collection | DOAJ |
| description | Background/Purpose: Spinal cord injury (SCI) is a devastating medical condition for which no effective pharmacological interventions exist. l-Theanine (LT), a major amino acid component of green tea, exhibits potent antioxidative and anti-inflammatory activities and protects against various neural injuries. Here, we evaluated the potential therapeutic effects of LT on the recovery of behavioral motor functions after SCI in rats and the underlying neuroprotective mechanisms. Methods: SCI was induced by applying vascular clips to the dura through a four-level T5–T8 laminectomy, and saline or LT (10/30 mg/kg) was intrathecally administered at 1-, 6-, and 24-h post-SCI. At 72-h post-SCI, half of the rats from each group for each parameter were sacrificed, and their spinal cord was excised for measurement of malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase, catalase, tumor necrosis factor-α, interleukin-1β/-6, myeloperoxidase, and caspase-3. The remaining rats from each group were subjected to Bresnahan locomotor-rating scale (BBB), inclined-plane, toe-spread, and hindfoot bar-grab tests at 1-, 4-, 7-, 10-, and 14-days post-SCI. Results: LT treatment reduced NO and MDA levels, increased antioxidative strength, and markedly suppressed the levels of neuroinflammatory and apoptotic markers in the spinal cord after SCI. Moreover, LT treatment drastically promoted the recovery of behavioral motor functions post-SCI. Conclusion: Our findings revealed that LT can enhance the recovery of behavioral motor functions after SCI in rats, which related to the suppression of post-traumatic oxidative response, neural inflammation, and apoptosis. This evidence indicates that LT holds considerable potential for use in the clinical treatment/prevention of SCI-induced motor dysfunction. |
| first_indexed | 2024-12-10T22:02:26Z |
| format | Article |
| id | doaj.art-a0a5776d4216482d9de0d22f3aebfd08 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-10T22:02:26Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-a0a5776d4216482d9de0d22f3aebfd082022-12-22T01:31:51ZengElsevierJournal of the Formosan Medical Association0929-66462020-09-01119914051414l-Theanine improves functional recovery after traumatic spinal cord injury in ratsChih-Chuan Yang0Kuo-Chi Chang1Mao-Hsien Wang2Hsiang-Chien Tseng3Hung-Sheng Soung4Chih-Hsiang Fang5Yi-Wen Lin6Keng-Yuan Li7Cheng-Chia Tsai8Department of Neurosurgery, Mackay Memorial Hospital, Taipei, 10449, Taiwan, ROC; Department of Medicine, Mackay Medical College, New Taipei City, 252, Taiwan, ROCDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, 10608, Taiwan, ROCDepartment of Anesthesia, En Chu Kon Hospital, Sanshia District, New Taipei City, 23702, Taiwan, ROCDepartment of Anesthesiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, 11101, Taiwan, ROC; School of Medicine, Fu Jen Catholic University, New Taipei City, 24205, Taiwan, ROCDepartment of Psychiatry, Yuan-Shan Br. of Taipei Veteran General Hospital, Yilan County, 26604, Taiwan, ROC; Department of Biomedical Engineering, National Defense Medical Center, Taipei, 11490, Taiwan, ROCInstitute of Biomedical Engineering, National Taiwan University, Taipei, 10051, Taiwan, ROCInstitute of Biomedical Engineering, National Taiwan University, Taipei, 10051, Taiwan, ROCInstitute of Biomedical Engineering, National Taiwan University, Taipei, 10051, Taiwan, ROCDepartment of Neurosurgery, Mackay Memorial Hospital, Taipei, 10449, Taiwan, ROC; Department of Medicine, Mackay Medical College, New Taipei City, 252, Taiwan, ROC; Corresponding author. Department of Neurosurgery, Mackay Memorial Hospital, No. 92, Sec. 2, Zhongshan N. Rd., Taipei City, 10449, Taiwan, ROC. Fax: +886 2 2543 3642.Background/Purpose: Spinal cord injury (SCI) is a devastating medical condition for which no effective pharmacological interventions exist. l-Theanine (LT), a major amino acid component of green tea, exhibits potent antioxidative and anti-inflammatory activities and protects against various neural injuries. Here, we evaluated the potential therapeutic effects of LT on the recovery of behavioral motor functions after SCI in rats and the underlying neuroprotective mechanisms. Methods: SCI was induced by applying vascular clips to the dura through a four-level T5–T8 laminectomy, and saline or LT (10/30 mg/kg) was intrathecally administered at 1-, 6-, and 24-h post-SCI. At 72-h post-SCI, half of the rats from each group for each parameter were sacrificed, and their spinal cord was excised for measurement of malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase, catalase, tumor necrosis factor-α, interleukin-1β/-6, myeloperoxidase, and caspase-3. The remaining rats from each group were subjected to Bresnahan locomotor-rating scale (BBB), inclined-plane, toe-spread, and hindfoot bar-grab tests at 1-, 4-, 7-, 10-, and 14-days post-SCI. Results: LT treatment reduced NO and MDA levels, increased antioxidative strength, and markedly suppressed the levels of neuroinflammatory and apoptotic markers in the spinal cord after SCI. Moreover, LT treatment drastically promoted the recovery of behavioral motor functions post-SCI. Conclusion: Our findings revealed that LT can enhance the recovery of behavioral motor functions after SCI in rats, which related to the suppression of post-traumatic oxidative response, neural inflammation, and apoptosis. This evidence indicates that LT holds considerable potential for use in the clinical treatment/prevention of SCI-induced motor dysfunction.http://www.sciencedirect.com/science/article/pii/S0929664619306047ApoptosisInflammationl-theanineOxidative stressSpinal cord injury |
| spellingShingle | Chih-Chuan Yang Kuo-Chi Chang Mao-Hsien Wang Hsiang-Chien Tseng Hung-Sheng Soung Chih-Hsiang Fang Yi-Wen Lin Keng-Yuan Li Cheng-Chia Tsai l-Theanine improves functional recovery after traumatic spinal cord injury in rats Journal of the Formosan Medical Association Apoptosis Inflammation l-theanine Oxidative stress Spinal cord injury |
| title | l-Theanine improves functional recovery after traumatic spinal cord injury in rats |
| title_full | l-Theanine improves functional recovery after traumatic spinal cord injury in rats |
| title_fullStr | l-Theanine improves functional recovery after traumatic spinal cord injury in rats |
| title_full_unstemmed | l-Theanine improves functional recovery after traumatic spinal cord injury in rats |
| title_short | l-Theanine improves functional recovery after traumatic spinal cord injury in rats |
| title_sort | l theanine improves functional recovery after traumatic spinal cord injury in rats |
| topic | Apoptosis Inflammation l-theanine Oxidative stress Spinal cord injury |
| url | http://www.sciencedirect.com/science/article/pii/S0929664619306047 |
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