Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water
Project Aquafluosense is designed to develop prototypes for a fluorescence-based instrumentation setup for in situ measurements of several characteristic parameters of water quality. In the scope of the project an enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the detect...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/3/182 |
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| author | Borbála Gémes Eszter Takács Patrik Gádoros Attila Barócsi László Kocsányi Sándor Lenk Attila Csákányi Szabolcs Kautny László Domján Gábor Szarvas Nóra Adányi Alexei Nabok Mária Mörtl András Székács |
| author_facet | Borbála Gémes Eszter Takács Patrik Gádoros Attila Barócsi László Kocsányi Sándor Lenk Attila Csákányi Szabolcs Kautny László Domján Gábor Szarvas Nóra Adányi Alexei Nabok Mária Mörtl András Székács |
| author_sort | Borbála Gémes |
| collection | DOAJ |
| description | Project Aquafluosense is designed to develop prototypes for a fluorescence-based instrumentation setup for in situ measurements of several characteristic parameters of water quality. In the scope of the project an enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the detection of several environmental xenobiotics, including mycotoxin zearalenone (ZON). ZON, produced by several plant pathogenic <i>Fusarium</i> species, has recently been identified as an emerging pollutant in surface water, presenting a hazard to aquatic ecosystems. Due to its physico-chemical properties, detection of ZON at low concentrations in surface water is a challenging task. The 96-well microplate-based fluorescence instrument is capable of detecting ZON in the concentration range of 0.09–400 ng/mL. The sensitivity and accuracy of the analytical method has been demonstrated by a comparative assessment with detection by high-performance liquid chromatography and by total internal reflection ellipsometry. The limit of detection of the method, 0.09 ng/mL, falls in the low range compared to the other reported immunoassays, but the main advantage of this ELFIA method is its efficacy in combined in situ applications for determination of various important water quality parameters detectable by induced fluorimerty—e.g., total organic carbon content, algal density or the level of other organic micropollutants detectable by immunofluorimetry. In addition, the immunofluorescence module can readily be expanded to other target analytes if proper antibodies are available for detection. |
| first_indexed | 2024-03-09T06:00:22Z |
| format | Article |
| id | doaj.art-a0abc4944e8a45589f11aee27fa44ae5 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T06:00:22Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-a0abc4944e8a45589f11aee27fa44ae52023-12-03T12:09:55ZengMDPI AGToxins2072-66512021-03-0113318210.3390/toxins13030182Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in WaterBorbála Gémes0Eszter Takács1Patrik Gádoros2Attila Barócsi3László Kocsányi4Sándor Lenk5Attila Csákányi6Szabolcs Kautny7László Domján8Gábor Szarvas9Nóra Adányi10Alexei Nabok11Mária Mörtl12András Székács13Agro-Environmental Research Centre, Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Herman O. út 15, H-1022 Budapest, HungaryAgro-Environmental Research Centre, Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Herman O. út 15, H-1022 Budapest, HungaryDepartment of Atomic Physics, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, HungaryDepartment of Atomic Physics, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, HungaryDepartment of Atomic Physics, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, HungaryDepartment of Atomic Physics, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, HungaryOptimal Optik Ltd., Dayka Gábor u. 6/B, H-1118 Budapest, HungaryOptimal Optik Ltd., Dayka Gábor u. 6/B, H-1118 Budapest, HungaryOptimal Optik Ltd., Dayka Gábor u. 6/B, H-1118 Budapest, HungaryOptimal Optik Ltd., Dayka Gábor u. 6/B, H-1118 Budapest, HungaryFood Science Research Centre, Institute of Food Sciences, Hungarian University of Agriculture and Life Sciences, Herman O. út 15, H-1022 Budapest, HungaryMaterials and Engineering Research Institute, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, UKAgro-Environmental Research Centre, Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Herman O. út 15, H-1022 Budapest, HungaryAgro-Environmental Research Centre, Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Herman O. út 15, H-1022 Budapest, HungaryProject Aquafluosense is designed to develop prototypes for a fluorescence-based instrumentation setup for in situ measurements of several characteristic parameters of water quality. In the scope of the project an enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the detection of several environmental xenobiotics, including mycotoxin zearalenone (ZON). ZON, produced by several plant pathogenic <i>Fusarium</i> species, has recently been identified as an emerging pollutant in surface water, presenting a hazard to aquatic ecosystems. Due to its physico-chemical properties, detection of ZON at low concentrations in surface water is a challenging task. The 96-well microplate-based fluorescence instrument is capable of detecting ZON in the concentration range of 0.09–400 ng/mL. The sensitivity and accuracy of the analytical method has been demonstrated by a comparative assessment with detection by high-performance liquid chromatography and by total internal reflection ellipsometry. The limit of detection of the method, 0.09 ng/mL, falls in the low range compared to the other reported immunoassays, but the main advantage of this ELFIA method is its efficacy in combined in situ applications for determination of various important water quality parameters detectable by induced fluorimerty—e.g., total organic carbon content, algal density or the level of other organic micropollutants detectable by immunofluorimetry. In addition, the immunofluorescence module can readily be expanded to other target analytes if proper antibodies are available for detection.https://www.mdpi.com/2072-6651/13/3/182zearalenonemycotoxincompetitive immunoassayfluorescence detectionhigh-performance liquid chromatographytotal internal reflection ellipsometry |
| spellingShingle | Borbála Gémes Eszter Takács Patrik Gádoros Attila Barócsi László Kocsányi Sándor Lenk Attila Csákányi Szabolcs Kautny László Domján Gábor Szarvas Nóra Adányi Alexei Nabok Mária Mörtl András Székács Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water Toxins zearalenone mycotoxin competitive immunoassay fluorescence detection high-performance liquid chromatography total internal reflection ellipsometry |
| title | Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water |
| title_full | Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water |
| title_fullStr | Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water |
| title_full_unstemmed | Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water |
| title_short | Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water |
| title_sort | development of an immunofluorescence assay module for determination of the mycotoxin zearalenone in water |
| topic | zearalenone mycotoxin competitive immunoassay fluorescence detection high-performance liquid chromatography total internal reflection ellipsometry |
| url | https://www.mdpi.com/2072-6651/13/3/182 |
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