GLI3 Is Required for OLIG2+ Progeny Production in Adult Dorsal Neural Stem Cells
The ventricular–subventricular zone (V-SVZ) is a postnatal germinal niche. It holds a large population of neural stem cells (NSCs) that generate neurons and oligodendrocytes for the olfactory bulb and (primarily) the corpus callosum, respectively. These NSCs are heterogeneous and generate different...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/2/218 |
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| author | Rebecca J. Embalabala Asa A. Brockman Amanda R. Jurewicz Jennifer A. Kong Kaitlyn Ryan Cristina D. Guinto Arturo Álvarez-Buylla Chin Chiang Rebecca A. Ihrie |
| author_facet | Rebecca J. Embalabala Asa A. Brockman Amanda R. Jurewicz Jennifer A. Kong Kaitlyn Ryan Cristina D. Guinto Arturo Álvarez-Buylla Chin Chiang Rebecca A. Ihrie |
| author_sort | Rebecca J. Embalabala |
| collection | DOAJ |
| description | The ventricular–subventricular zone (V-SVZ) is a postnatal germinal niche. It holds a large population of neural stem cells (NSCs) that generate neurons and oligodendrocytes for the olfactory bulb and (primarily) the corpus callosum, respectively. These NSCs are heterogeneous and generate different types of neurons depending on their location. Positional identity among NSCs is thought to be controlled in part by intrinsic pathways. However, extrinsic cell signaling through the secreted ligand Sonic hedgehog (Shh) is essential for neurogenesis in both the dorsal and ventral V-SVZ. Here we used a genetic approach to investigate the role of the transcription factors GLI2 and GLI3 in the proliferation and cell fate of dorsal and ventral V-SVZ NSCs. We find that while GLI3 is expressed in stem cell cultures from both dorsal and ventral V-SVZ, the repressor form of GLI3 is more abundant in dorsal V-SVZ. Despite this high dorsal expression and the requirement for other Shh pathway members, GLI3 loss affects the generation of ventrally-, but not dorsally-derived olfactory interneurons in vivo and does not affect trilineage differentiation in vitro. However, loss of GLI3 in the adult dorsal V-SVZ in vivo results in decreased numbers of OLIG2-expressing progeny, indicating a role in gliogenesis. |
| first_indexed | 2024-03-10T01:44:26Z |
| format | Article |
| id | doaj.art-a0ae46c327a44206b07e0cfd7199e004 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T01:44:26Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-a0ae46c327a44206b07e0cfd7199e0042023-11-23T13:17:53ZengMDPI AGCells2073-44092022-01-0111221810.3390/cells11020218GLI3 Is Required for OLIG2+ Progeny Production in Adult Dorsal Neural Stem CellsRebecca J. Embalabala0Asa A. Brockman1Amanda R. Jurewicz2Jennifer A. Kong3Kaitlyn Ryan4Cristina D. Guinto5Arturo Álvarez-Buylla6Chin Chiang7Rebecca A. Ihrie8Department of Cell & Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USADepartment of Cell & Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USADepartment of Cell & Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USADepartment of Cell & Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USADepartment of Cell & Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USADepartment of Neurological Surgery, University of California—San Francisco, San Francisco, CA 94143, USADepartment of Neurological Surgery, University of California—San Francisco, San Francisco, CA 94143, USADepartment of Cell & Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USADepartment of Cell & Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USAThe ventricular–subventricular zone (V-SVZ) is a postnatal germinal niche. It holds a large population of neural stem cells (NSCs) that generate neurons and oligodendrocytes for the olfactory bulb and (primarily) the corpus callosum, respectively. These NSCs are heterogeneous and generate different types of neurons depending on their location. Positional identity among NSCs is thought to be controlled in part by intrinsic pathways. However, extrinsic cell signaling through the secreted ligand Sonic hedgehog (Shh) is essential for neurogenesis in both the dorsal and ventral V-SVZ. Here we used a genetic approach to investigate the role of the transcription factors GLI2 and GLI3 in the proliferation and cell fate of dorsal and ventral V-SVZ NSCs. We find that while GLI3 is expressed in stem cell cultures from both dorsal and ventral V-SVZ, the repressor form of GLI3 is more abundant in dorsal V-SVZ. Despite this high dorsal expression and the requirement for other Shh pathway members, GLI3 loss affects the generation of ventrally-, but not dorsally-derived olfactory interneurons in vivo and does not affect trilineage differentiation in vitro. However, loss of GLI3 in the adult dorsal V-SVZ in vivo results in decreased numbers of OLIG2-expressing progeny, indicating a role in gliogenesis.https://www.mdpi.com/2073-4409/11/2/218ventricular–subventricular zone (V-SVZ)neurogenesisSonic hedgehog (Shh)oligodendrocytesGli3radial glial cells |
| spellingShingle | Rebecca J. Embalabala Asa A. Brockman Amanda R. Jurewicz Jennifer A. Kong Kaitlyn Ryan Cristina D. Guinto Arturo Álvarez-Buylla Chin Chiang Rebecca A. Ihrie GLI3 Is Required for OLIG2+ Progeny Production in Adult Dorsal Neural Stem Cells Cells ventricular–subventricular zone (V-SVZ) neurogenesis Sonic hedgehog (Shh) oligodendrocytes Gli3 radial glial cells |
| title | GLI3 Is Required for OLIG2+ Progeny Production in Adult Dorsal Neural Stem Cells |
| title_full | GLI3 Is Required for OLIG2+ Progeny Production in Adult Dorsal Neural Stem Cells |
| title_fullStr | GLI3 Is Required for OLIG2+ Progeny Production in Adult Dorsal Neural Stem Cells |
| title_full_unstemmed | GLI3 Is Required for OLIG2+ Progeny Production in Adult Dorsal Neural Stem Cells |
| title_short | GLI3 Is Required for OLIG2+ Progeny Production in Adult Dorsal Neural Stem Cells |
| title_sort | gli3 is required for olig2 progeny production in adult dorsal neural stem cells |
| topic | ventricular–subventricular zone (V-SVZ) neurogenesis Sonic hedgehog (Shh) oligodendrocytes Gli3 radial glial cells |
| url | https://www.mdpi.com/2073-4409/11/2/218 |
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