Correction of the weight and length for juveniles Atherinella brasiliensis (Actinopterygii: Atherinopsidae) after fixation in formalin and preservation in ethanol
The present study compared losses of weight and length in specimens of Atherinella brasiliensis (Quoy & Gaimard, 1825) after fixation in formalin 10% during ten days, and posterior preservation in ethanol 70% for 45 days, in two solvents: freshwater and marine water. Additionally, correction fac...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Zoologia
2010-01-01
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| Series: | Zoologia (Curitiba) |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000600009 |
| _version_ | 1828077503590170624 |
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| author | Marcelo T. Melo Cíntia Saturnino Joaquim N. S. Santos Ruan M. Vasconcellos Antonio G. Cruz-Filho Francisco G. Araújo |
| author_facet | Marcelo T. Melo Cíntia Saturnino Joaquim N. S. Santos Ruan M. Vasconcellos Antonio G. Cruz-Filho Francisco G. Araújo |
| author_sort | Marcelo T. Melo |
| collection | DOAJ |
| description | The present study compared losses of weight and length in specimens of Atherinella brasiliensis (Quoy & Gaimard, 1825) after fixation in formalin 10% during ten days, and posterior preservation in ethanol 70% for 45 days, in two solvents: freshwater and marine water. Additionally, correction factors were proposed to calculate the correction for weight and length from preserved specimens. The specimens were weighted and measured after collection (fresh), and with 10, 20, 27, 34, 41 and 55 days of fixation plus preservation. The largest losses in weight occurred during the first 20 days (10.35 ± 0.31% - freshwater; 11.29 ± 0.44% - marine water), continuing with lesser intensity up to the 27th day in freshwater dilution, and up to the 34th day in marine water dilution. Weight losses stabilized by the 34th day for both freshwater (11.77 ± 0.33%) and marine water (13.62 ± 0.41%). The largest losses in the total length for the two methods were also observed in the first 20 days (7.42 ± 0.27% - freshwater; 9.76 ± 0.26 - marine water), stabilizing after the 27th day in freshwater (8.60 ± 0.26%) and 20th day in marine water (9.76 ± 0.26%). The losses of weight and total length were significantly dependent on fish size, with smaller individuals suffering the most significant losses. Complete regression equations were proposed for the retro-calculation of fresh weight and total length from the preserved specimens for freshwater (Wfresh = 1.0536W P + 0.0416; TLfresh = 0.9588TL P + 5.8437) and marine water (Wfresh = 1.0868W P + 0.0451; TLfresh = 0.8621TL P + 10.425). |
| first_indexed | 2024-04-11T02:31:34Z |
| format | Article |
| id | doaj.art-a0aedc23854646dfa9a08d3dfe82f7b4 |
| institution | Directory Open Access Journal |
| issn | 1984-4670 1984-4689 |
| language | English |
| last_indexed | 2024-04-11T02:31:34Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Zoologia |
| record_format | Article |
| series | Zoologia (Curitiba) |
| spelling | doaj.art-a0aedc23854646dfa9a08d3dfe82f7b42023-01-02T21:23:46ZengSociedade Brasileira de ZoologiaZoologia (Curitiba)1984-46701984-46892010-01-0127689289610.1590/S1984-46702010000600009Correction of the weight and length for juveniles Atherinella brasiliensis (Actinopterygii: Atherinopsidae) after fixation in formalin and preservation in ethanolMarcelo T. MeloCíntia SaturninoJoaquim N. S. SantosRuan M. VasconcellosAntonio G. Cruz-FilhoFrancisco G. AraújoThe present study compared losses of weight and length in specimens of Atherinella brasiliensis (Quoy & Gaimard, 1825) after fixation in formalin 10% during ten days, and posterior preservation in ethanol 70% for 45 days, in two solvents: freshwater and marine water. Additionally, correction factors were proposed to calculate the correction for weight and length from preserved specimens. The specimens were weighted and measured after collection (fresh), and with 10, 20, 27, 34, 41 and 55 days of fixation plus preservation. The largest losses in weight occurred during the first 20 days (10.35 ± 0.31% - freshwater; 11.29 ± 0.44% - marine water), continuing with lesser intensity up to the 27th day in freshwater dilution, and up to the 34th day in marine water dilution. Weight losses stabilized by the 34th day for both freshwater (11.77 ± 0.33%) and marine water (13.62 ± 0.41%). The largest losses in the total length for the two methods were also observed in the first 20 days (7.42 ± 0.27% - freshwater; 9.76 ± 0.26 - marine water), stabilizing after the 27th day in freshwater (8.60 ± 0.26%) and 20th day in marine water (9.76 ± 0.26%). The losses of weight and total length were significantly dependent on fish size, with smaller individuals suffering the most significant losses. Complete regression equations were proposed for the retro-calculation of fresh weight and total length from the preserved specimens for freshwater (Wfresh = 1.0536W P + 0.0416; TLfresh = 0.9588TL P + 5.8437) and marine water (Wfresh = 1.0868W P + 0.0451; TLfresh = 0.8621TL P + 10.425).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000600009Back-calculationinitial lengthshrinkagesize conversion |
| spellingShingle | Marcelo T. Melo Cíntia Saturnino Joaquim N. S. Santos Ruan M. Vasconcellos Antonio G. Cruz-Filho Francisco G. Araújo Correction of the weight and length for juveniles Atherinella brasiliensis (Actinopterygii: Atherinopsidae) after fixation in formalin and preservation in ethanol Zoologia (Curitiba) Back-calculation initial length shrinkage size conversion |
| title | Correction of the weight and length for juveniles Atherinella brasiliensis (Actinopterygii: Atherinopsidae) after fixation in formalin and preservation in ethanol |
| title_full | Correction of the weight and length for juveniles Atherinella brasiliensis (Actinopterygii: Atherinopsidae) after fixation in formalin and preservation in ethanol |
| title_fullStr | Correction of the weight and length for juveniles Atherinella brasiliensis (Actinopterygii: Atherinopsidae) after fixation in formalin and preservation in ethanol |
| title_full_unstemmed | Correction of the weight and length for juveniles Atherinella brasiliensis (Actinopterygii: Atherinopsidae) after fixation in formalin and preservation in ethanol |
| title_short | Correction of the weight and length for juveniles Atherinella brasiliensis (Actinopterygii: Atherinopsidae) after fixation in formalin and preservation in ethanol |
| title_sort | correction of the weight and length for juveniles atherinella brasiliensis actinopterygii atherinopsidae after fixation in formalin and preservation in ethanol |
| topic | Back-calculation initial length shrinkage size conversion |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000600009 |
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