Application of Allele Specific PCR in Identifying Offspring Genotypes of Bi-Allelic <i>SbeIIb</i> Mutant Lines in Rice

Application of Allele Specific PCR in Identifying Offspring Genotypes of Bi-Allelic <i>SbeIIb</i> Mutant Lines in Rice

Bi-allelic mutant lines induced by clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated (Cas) systems are important genetic materials. It is very important to establish a rapid and cheap method in identifying homozygous mutant plants from offspring segregation populat...

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Bibliographic Details
Main Authors: Yongqi Jiang, Yinhui Ren, Xin Xu, Hao Wang, Cunxu Wei
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Plants
Subjects:
clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated (Cas) system
bi-allelic mutation line
allele specific PCR
genotype identification
Online Access:https://www.mdpi.com/2223-7747/11/4/524

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