Psychrophilic phage VSW-3 RNA polymerase reduces both terminal and full-length dsRNA byproducts in in vitro transcription

Psychrophilic phage VSW-3 RNA polymerase reduces both terminal and full-length dsRNA byproducts in in vitro transcription

RNA research and applications are underpinned by in vitro transcription (IVT), but RNA impurities resulting from the enzymatic reagents severely impede downstream applications. To improve the stability and purity of synthesized RNA, we have characterized a novel single-subunit RNA polymerase (RNAP)...

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Bibliographic Details
Main Authors: Heng Xia, Bingbing Yu, Yixin Jiang, Rui Cheng, Xueling Lu, Hui Wu, Bin Zhu
Format: Article
Language:English
Published: Taylor & Francis Group 2022-12-01
Series:RNA Biology
Subjects:
dsrna
sgrna
mrna medicine
t7 rna polymerase
in vitro transcription
Online Access:http://dx.doi.org/10.1080/15476286.2022.2139113

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