Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2)
Micropropagation techniques changed the production of clonal plantlets in the world. However, the high costs of micropropagated plantlets continue as the main constraint for the expansion of the technique. This paper aimed to test the use of the chemical sterilization of culture medium using chlori...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Floricultura e Plantas Ornamentais
2018-09-01
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| Series: | Ornamental Horticulture |
| Subjects: | |
| Online Access: | https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/1222 |
| _version_ | 1827918805415755776 |
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| author | Jean Carlos Cardoso Ana Carolina Petit Inthurn |
| author_facet | Jean Carlos Cardoso Ana Carolina Petit Inthurn |
| author_sort | Jean Carlos Cardoso |
| collection | DOAJ |
| description |
Micropropagation techniques changed the production of clonal plantlets in the world. However, the high costs of micropropagated plantlets continue as the main constraint for the expansion of the technique. This paper aimed to test the use of the chemical sterilization of culture medium using chlorine dioxide (ClO2) for in vitro cultivation of gerbera. There was used gerbera in vitro shoots in the stage of rooting for these experiments, using 0.0035%, 0.0070% and 0.0105% of chlorine dioxide in the culture medium. Also, peracetic acid was tested previously for sterilization, but resulted in microbial contamination. Chemical sterilization of the culture medium was successfully using ClO2 at 0.0035% to 0.0105% (100% decontamination) at rooting and elongation stage of gerbera with production of plantlets with similar (number of leaves, total and root fresh weight) or higher quality (mainly aerial part) at rooting/elongation stage, compared with autoclaved culture medium. The increase of concentration of ClO2 also resulted in increasing of height and fresh weight of aerial part of gerberas. The ClO2 could replace the autoclaving with production of sterilized culture medium without phytotoxic problems to gerbera in vitro cultivation.
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| first_indexed | 2024-03-13T03:49:03Z |
| format | Article |
| id | doaj.art-a0b747b563e048cfa4008526bdb1324a |
| institution | Directory Open Access Journal |
| issn | 2447-536X |
| language | English |
| last_indexed | 2024-03-13T03:49:03Z |
| publishDate | 2018-09-01 |
| publisher | Sociedade Brasileira de Floricultura e Plantas Ornamentais |
| record_format | Article |
| series | Ornamental Horticulture |
| spelling | doaj.art-a0b747b563e048cfa4008526bdb1324a2023-06-22T20:35:09ZengSociedade Brasileira de Floricultura e Plantas OrnamentaisOrnamental Horticulture2447-536X2018-09-0124310.14295/oh.v24i3.1222953Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2)Jean Carlos CardosoAna Carolina Petit Inthurn0Monsanto Micropropagation techniques changed the production of clonal plantlets in the world. However, the high costs of micropropagated plantlets continue as the main constraint for the expansion of the technique. This paper aimed to test the use of the chemical sterilization of culture medium using chlorine dioxide (ClO2) for in vitro cultivation of gerbera. There was used gerbera in vitro shoots in the stage of rooting for these experiments, using 0.0035%, 0.0070% and 0.0105% of chlorine dioxide in the culture medium. Also, peracetic acid was tested previously for sterilization, but resulted in microbial contamination. Chemical sterilization of the culture medium was successfully using ClO2 at 0.0035% to 0.0105% (100% decontamination) at rooting and elongation stage of gerbera with production of plantlets with similar (number of leaves, total and root fresh weight) or higher quality (mainly aerial part) at rooting/elongation stage, compared with autoclaved culture medium. The increase of concentration of ClO2 also resulted in increasing of height and fresh weight of aerial part of gerberas. The ClO2 could replace the autoclaving with production of sterilized culture medium without phytotoxic problems to gerbera in vitro cultivation. https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/1222Gerbera jamesoniimicropropagationsterilization methodscost reductionchemical alterationsphytotoxicity. |
| spellingShingle | Jean Carlos Cardoso Ana Carolina Petit Inthurn Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) Ornamental Horticulture Gerbera jamesonii micropropagation sterilization methods cost reduction chemical alterations phytotoxicity. |
| title | Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
| title_full | Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
| title_fullStr | Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
| title_full_unstemmed | Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
| title_short | Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2) |
| title_sort | easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide clo2 |
| topic | Gerbera jamesonii micropropagation sterilization methods cost reduction chemical alterations phytotoxicity. |
| url | https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/1222 |
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