n-3 PUFAs enhance the frequency of murine B-cell subsets and restore the impairment of antibody production to a T-independent antigen in obesity[S]
The role of n-3 polyunsaturated fatty acids (PUFA) on in vivo B-cell immunity is unknown. We first investigated how n-3 PUFAs impacted in vivo B-cell phenotypes and antibody production in the absence and presence of antigen compared with a control diet. Lean mice consuming n-3 PUFAs for 4 weeks disp...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520350641 |
| _version_ | 1818727912899084288 |
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| author | Heather Teague Cassie J. Fhaner Mitchel Harris David M. Duriancik Gavin E. Reid Saame Raza Shaikh |
| author_facet | Heather Teague Cassie J. Fhaner Mitchel Harris David M. Duriancik Gavin E. Reid Saame Raza Shaikh |
| author_sort | Heather Teague |
| collection | DOAJ |
| description | The role of n-3 polyunsaturated fatty acids (PUFA) on in vivo B-cell immunity is unknown. We first investigated how n-3 PUFAs impacted in vivo B-cell phenotypes and antibody production in the absence and presence of antigen compared with a control diet. Lean mice consuming n-3 PUFAs for 4 weeks displayed increased percentage and frequency of splenic transitional 1 B cells. Upon stimulation with trinitrophenylated-lipopolysaccharide, n-3 PUFAs increased the number of splenic transitional 1/2, follicular, premarginal, and marginal zone B cells. n-3 PUFAs also increased surface, but not circulating, IgM. We next tested the effects of n-3 PUFAs in a model of obesity that is associated with suppressed humoral immunity. An obesogenic diet after ten weeks of feeding, relative to a lean control, had no effect on the frequency of B cells but lowered circulating IgM upon antigen stimulation. Administration of n-3 PUFAs to lean and obese mice increased the percentage and/or frequency of transitional 1 and marginal zone B cells. Furthermore, n-3 PUFAs in lean and obese mice increased circulating IgM relative to controls. Altogether, the data show n-3 PUFAs enhance B cell-mediated immunity in vivo, which has implications for immunocompromised populations, such as the obese. |
| first_indexed | 2024-12-17T22:21:39Z |
| format | Article |
| id | doaj.art-a0b77a898a5b447788e473e4b36c2cd5 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T22:21:39Z |
| publishDate | 2013-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-a0b77a898a5b447788e473e4b36c2cd52022-12-21T21:30:28ZengElsevierJournal of Lipid Research0022-22752013-11-01541131303138n-3 PUFAs enhance the frequency of murine B-cell subsets and restore the impairment of antibody production to a T-independent antigen in obesity[S]Heather Teague0Cassie J. Fhaner1Mitchel Harris2David M. Duriancik3Gavin E. Reid4Saame Raza Shaikh5Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NCDepartments of Chemistry, Michigan State University, East Lansing, MIDepartment of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NCFood Science and Nutrition, Michigan State University, East Lansing, MIDepartments of Chemistry, Michigan State University, East Lansing, MI; Biochemistry and Molecular Biology, Michigan State University, East Lansing, MIDepartment of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC; To whom correspondence should be addressedThe role of n-3 polyunsaturated fatty acids (PUFA) on in vivo B-cell immunity is unknown. We first investigated how n-3 PUFAs impacted in vivo B-cell phenotypes and antibody production in the absence and presence of antigen compared with a control diet. Lean mice consuming n-3 PUFAs for 4 weeks displayed increased percentage and frequency of splenic transitional 1 B cells. Upon stimulation with trinitrophenylated-lipopolysaccharide, n-3 PUFAs increased the number of splenic transitional 1/2, follicular, premarginal, and marginal zone B cells. n-3 PUFAs also increased surface, but not circulating, IgM. We next tested the effects of n-3 PUFAs in a model of obesity that is associated with suppressed humoral immunity. An obesogenic diet after ten weeks of feeding, relative to a lean control, had no effect on the frequency of B cells but lowered circulating IgM upon antigen stimulation. Administration of n-3 PUFAs to lean and obese mice increased the percentage and/or frequency of transitional 1 and marginal zone B cells. Furthermore, n-3 PUFAs in lean and obese mice increased circulating IgM relative to controls. Altogether, the data show n-3 PUFAs enhance B cell-mediated immunity in vivo, which has implications for immunocompromised populations, such as the obese.http://www.sciencedirect.com/science/article/pii/S0022227520350641humoral immunityeicosapentaenoic aciddocosahexaenoic acid |
| spellingShingle | Heather Teague Cassie J. Fhaner Mitchel Harris David M. Duriancik Gavin E. Reid Saame Raza Shaikh n-3 PUFAs enhance the frequency of murine B-cell subsets and restore the impairment of antibody production to a T-independent antigen in obesity[S] Journal of Lipid Research humoral immunity eicosapentaenoic acid docosahexaenoic acid |
| title | n-3 PUFAs enhance the frequency of murine B-cell subsets and restore the impairment of antibody production to a T-independent antigen in obesity[S] |
| title_full | n-3 PUFAs enhance the frequency of murine B-cell subsets and restore the impairment of antibody production to a T-independent antigen in obesity[S] |
| title_fullStr | n-3 PUFAs enhance the frequency of murine B-cell subsets and restore the impairment of antibody production to a T-independent antigen in obesity[S] |
| title_full_unstemmed | n-3 PUFAs enhance the frequency of murine B-cell subsets and restore the impairment of antibody production to a T-independent antigen in obesity[S] |
| title_short | n-3 PUFAs enhance the frequency of murine B-cell subsets and restore the impairment of antibody production to a T-independent antigen in obesity[S] |
| title_sort | n 3 pufas enhance the frequency of murine b cell subsets and restore the impairment of antibody production to a t independent antigen in obesity s |
| topic | humoral immunity eicosapentaenoic acid docosahexaenoic acid |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520350641 |
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