The Antimicrobial Potential and Aquaculture Wastewater Treatment Ability of Penaeidins 3a Transgenic Duckweed
With the development of aquaculture, wastewater treatment and diseases have been paid more and more attention. The question of how to improve the immunity of aquatic species, as well as treat aquaculture wastewater, has become an urgent problem. In this study, duckweed with a high protein content (3...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/8/1715 |
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| author | Lin Yang Ximeng Luo Jinge Sun Xu Ma Qiuting Ren Yaya Wang Wenqiao Wang Yuman He Qingqing Li Bing Han Yiqi Yu Jinsheng Sun |
| author_facet | Lin Yang Ximeng Luo Jinge Sun Xu Ma Qiuting Ren Yaya Wang Wenqiao Wang Yuman He Qingqing Li Bing Han Yiqi Yu Jinsheng Sun |
| author_sort | Lin Yang |
| collection | DOAJ |
| description | With the development of aquaculture, wastewater treatment and diseases have been paid more and more attention. The question of how to improve the immunity of aquatic species, as well as treat aquaculture wastewater, has become an urgent problem. In this study, duckweed with a high protein content (37.4%) (<i>Lemna turionifera</i> 5511) has been employed as a feedstock for aquatic wastewater treatment and the production of antimicrobial peptides. Penaeidins 3a (Pen3a), from <i>Litopenaeus vannamei</i>, were expressed under the control of CaMV-35S promoter in duckweed. Bacteriostatic testing using the Pen3a duckweed extract showed its antibacterial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. Transcriptome analysis of wild type (WT) duckweed and Pen3a duckweed showed different results, and the protein metabolic process was the most up-regulated by differential expression genes (DEGs). In Pen3a transgenic duckweed, the expression of sphingolipid metabolism and phagocytosis process-related genes have been significantly up-regulated. Quantitative proteomics suggested a remarkable difference in protein enrichment in the metabolic pathway. Pen3a duckweed decreased the bacterial number, and effectively inhibited the growth of <i>Nitrospirae.</i> Additionally, Pen3a duckweed displayed better growth in the lake. The study showed the nutritional and antibacterial value of duckweed as an animal feed ingredient. |
| first_indexed | 2024-03-11T04:36:23Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T04:36:23Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-a0b8da6da8f84e80baff6f1c5c02f1192023-11-17T21:00:38ZengMDPI AGPlants2223-77472023-04-01128171510.3390/plants12081715The Antimicrobial Potential and Aquaculture Wastewater Treatment Ability of Penaeidins 3a Transgenic DuckweedLin Yang0Ximeng Luo1Jinge Sun2Xu Ma3Qiuting Ren4Yaya Wang5Wenqiao Wang6Yuman He7Qingqing Li8Bing Han9Yiqi Yu10Jinsheng Sun11Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, ChinaTianjin Nankai Xiangyu School, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, ChinaWith the development of aquaculture, wastewater treatment and diseases have been paid more and more attention. The question of how to improve the immunity of aquatic species, as well as treat aquaculture wastewater, has become an urgent problem. In this study, duckweed with a high protein content (37.4%) (<i>Lemna turionifera</i> 5511) has been employed as a feedstock for aquatic wastewater treatment and the production of antimicrobial peptides. Penaeidins 3a (Pen3a), from <i>Litopenaeus vannamei</i>, were expressed under the control of CaMV-35S promoter in duckweed. Bacteriostatic testing using the Pen3a duckweed extract showed its antibacterial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. Transcriptome analysis of wild type (WT) duckweed and Pen3a duckweed showed different results, and the protein metabolic process was the most up-regulated by differential expression genes (DEGs). In Pen3a transgenic duckweed, the expression of sphingolipid metabolism and phagocytosis process-related genes have been significantly up-regulated. Quantitative proteomics suggested a remarkable difference in protein enrichment in the metabolic pathway. Pen3a duckweed decreased the bacterial number, and effectively inhibited the growth of <i>Nitrospirae.</i> Additionally, Pen3a duckweed displayed better growth in the lake. The study showed the nutritional and antibacterial value of duckweed as an animal feed ingredient.https://www.mdpi.com/2223-7747/12/8/1715duckweedspenaeidinsbacteria inhibitiontranscriptomicsquantitative proteomics |
| spellingShingle | Lin Yang Ximeng Luo Jinge Sun Xu Ma Qiuting Ren Yaya Wang Wenqiao Wang Yuman He Qingqing Li Bing Han Yiqi Yu Jinsheng Sun The Antimicrobial Potential and Aquaculture Wastewater Treatment Ability of Penaeidins 3a Transgenic Duckweed Plants duckweeds penaeidins bacteria inhibition transcriptomics quantitative proteomics |
| title | The Antimicrobial Potential and Aquaculture Wastewater Treatment Ability of Penaeidins 3a Transgenic Duckweed |
| title_full | The Antimicrobial Potential and Aquaculture Wastewater Treatment Ability of Penaeidins 3a Transgenic Duckweed |
| title_fullStr | The Antimicrobial Potential and Aquaculture Wastewater Treatment Ability of Penaeidins 3a Transgenic Duckweed |
| title_full_unstemmed | The Antimicrobial Potential and Aquaculture Wastewater Treatment Ability of Penaeidins 3a Transgenic Duckweed |
| title_short | The Antimicrobial Potential and Aquaculture Wastewater Treatment Ability of Penaeidins 3a Transgenic Duckweed |
| title_sort | antimicrobial potential and aquaculture wastewater treatment ability of penaeidins 3a transgenic duckweed |
| topic | duckweeds penaeidins bacteria inhibition transcriptomics quantitative proteomics |
| url | https://www.mdpi.com/2223-7747/12/8/1715 |
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